A visual reporter system for virus-induced gene silencing in tomato fruit based on anthocyanin accumulation

Plant Physiology

Volumn 150, Issue 3, 2009, Pages 1122-1134

  Orzaez, Diego ^a   Medina, Aurora ^a   Torre, Sara ^a,c   Fernández Moreno, Josefina Patricia ^a   Rambla, José Luis ^a   Fernández del Carmen, Asun ^a   Butelli, Eugenio ^b   Martin, Cathie ^b   Granell, Antonio ^a  

^a Ciudad Politécnica de la Innovación   (Spain)

^b JOHN INNES CENTRE   (United Kingdom)

^c UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIRRHINUM; ANTIRRHINUM MAJUS; LYCOPERSICON ESCULENTUM; NICOTIANA TABACUM; PETUNIA; PETUNIA X HYBRIDA; SOLANUM; TOBACCO RATTLE VIRUS;

EID: 67650160040     PISSN: 00320889     EISSN: 15322548     Source Type: Journal    
DOI: 10.1104/pp.109.139006     Document Type: Article

References (39)

1. Brigneti G, Martin-Hernandez AM, Jin HL, Chen J, Baulcombe DC, Baker B, Jones JDG (2004) Virus-induced gene silencing in Solanum species. Plant J 39: 264-272
2. Burch-Smith TM, Schiff M, Liu YL, Dinesh-Kumar SP (2006) Efficient virus-induced gene silencing in Arabidopsis. Plant Physiol 142: 21-27
3. Butelli E, Titta L, Giorgio M, Mock HP, Matros A, Peterek S, Schijlen EG, Hall RD, Bovy AG, Luo J, Martin C (2008) Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors. Nat Biotechnol 26: 1301-1308
4. Chen GP, Hackett R, Walker D, Taylor A, Lin ZF, Grierson D (2004a) Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds. Plant Physiol 136: 2641-2651
5. Chen JC, Jiang CZ, Gookin TE, Hunter DA, Clark DG, Reid MS (2004b) Chalcone synthase as a reporter in virus-induced gene silencing studies of flower senescence. Plant Mol Biol 55: 521-530
6. Dinesh-Kumar SP, Burch-Smith T, Liu Y, Schiff M, Dong Y, Zhu X, Mamillapalli P (2007) Virus-induced gene silencing (VIGS) for gene function studies in plants. Phytopathology 97: S145
7. Dong Y, Burch-Smith TM, Liu Y, Mamillapalli P, Dinesh-Kumar SP (2007) A ligation-independent cloning tobacco rattle virus vector for high-throughput virus-induced gene silencing identifies roles for NbMADS4-1 and -2 in floral development. Plant Physiol 145: 1161-1170
8. Fu DQ, Zhu BZ, Zhu HL, Jiang WB, Luo YB (2005) Virus-induced gene silencing in tomato fruit. Plant J 43: 299-308
9. Fu DQ, Zhu BZ, Zhu HL, Zhang HX, Xie YH, Jiang WB, Zhao XD, Luo YB (2006) Enhancement of virus-induced gene silencing in tomato by low temperature and low humidity. Mol Cells 21: 153-160
10. Hoffmann T, Kalinowski G, Schwab W (2006) RNAi-induced silencing of gene expression in strawberry fruit (Fragaria x ananassa) by agroinfiltration: a rapid assay for gene function analysis. Plant J 48: 818-826
11. Jiang CZ, Lu F, Imsabai W, Meir S, Reid MS (2008) Silencing polygalacturonase expression inhibits tomato petiole abscission. J Exp Bot 59: 973-979
12. Kruger NJ, Troncoso-Ponce MA, Ratcliffe RG (2008) 1H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues. Nat Protocols 3: 1001-1012
13. Lee J, Durst RW, Wrolstad RE (2005) Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. J AOAC Int 88: 1269-1278
14. Lewinsohn E, Sitrit Y, Bar E, Azulay Y, Ibdah M, Meir A, Yosef E, Zamir D, Tadmor Y (2005a) Not just colors: carotenoid degradation as a link between pigmentation and aroma in tomato and watermelon fruit. Trends Food Sci Technol 16: 407-415
15. Lewinsohn E, Sitrit Y, Bar E, Azulay Y, Meir A, Zamir D, Tadmor Y (2005b) Carotenoid pigmentation affects the volatile composition of tomato and watermelon fruits, as revealed by comparative genetic analyses. J Agric Food Chem 53: 3142-3148
16. Liu YL, Schiff M, Dinesh-Kumar SP (2002) Virus-induced gene silencing in tomato. Plant J 31: 777-786
17. Luo J, Butelli E, Hill L, Parr A, Niggeweg R, Bailey P, Weisshaar B, Martin C (2008) AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato: expression in fruit results in very high levels of both types of polyphenol. Plant J 56: 316-326
18. Marti C, Orzaez D, Ellul P, Moreno V, Carbonell J, Granell A (2007) Silencing of DELLA induces facultative parthenocarpy in tomato fruits. Plant J 52: 865-876
19. Martin C, Paz-Ares J (1997) MYB transcription factors in plants. Trends Genet 13: 67-73
20. Mathieu S, Cin VD, Fei Z, Li H, Bliss P, Taylor MG, Klee HJ, Tieman DM (2009) Flavour compounds in tomato fruits: identification of loci and potential pathways affecting volatile composition. J Exp Bot 60: 325-337
21. Mehrtens F, Kranz H, Bednarek P, Weisshaar B (2005) The Arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiol 138: 1083-1096
22. Nagamatsu A, Masuta C, Senda M, Matsuura H, Kasai A, Hong JS, Kitamura K, Abe J, Kanazawa A (2007) Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing. Plant Biotechnol J 5: 778-790
23. Nelson RS, Ding X, Ballard K, Chaluvadi SR (2007) Use of Brome mosaic virus for virus-induced gene silencing in rice, fescue and switchgrass. Phytopathology 97: S83
24. Orzaez D, Mirabel S, Wieland WH, Granell A (2006) Agroinjection of tomato fruits: a tool for rapid functional analysis of transgenes directly in fruit. Plant Physiol 140: 3-11
25. Ratcliff F, Martin-Hernandez AM, Baulcombe DC (2001) Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J 25: 237-245
26. Rotenberg D, Thompson TS, German TL, Willis DK (2006) Methods for effective real-time RT-PCR analysis of virus-induced gene silencing. J Virol Methods 138: 49-59
27. Ruiz MT, Voinnet O, Baulcombe DC (1998) Initiation and maintenance of virus-induced gene silencing. Plant Cell 10: 937-946
28. Ryu CM, Anand A, Kang L, Mysore KS (2004) Agrodrench: a novel and effective agroinoculation method for virus-induced gene silencing in roots and diverse solanaceous species. Plant J 40: 322-331
29. Schwinn K, Venail J, Shang YJ, Mackay S, Alm V, Butelli E, Oyama R, Bailey P, Davies K, Martin C (2006) A small family of MYB-regulatory genes controls floral pigmentation intensity and patterning in the genus Antirrhinum. Plant Cell 18: 831-851
30. Senthil-Kumar M, Hema R, Anand A, Kang L, Udayakumar M, Mysore KS (2007) A systematic study to determine the extent of gene silencing in Nicotiana benthamiana and other Solanaceae species when heterologous gene sequences are used for virus-induced gene silencing. New Phytol 176: 782-791
31. Spitzer B, Ben Zvi MM, Ovadis M, Marhevka E, Barkai O, Edelbaum O, Marton I, Masci T, Alon M, Morin S, et al (2007) Reverse genetics of floral scent: application of tobacco rattle virus-based gene silencing in petunia. Plant Physiol 145: 1241-1250
32. Tieman DM, Zeigler M, Schmelz EA, Taylor MG, Bliss P, Kirst M, Klee HJ (2006) Identification of loci affecting flavour volatile emissions in tomato fruits. J Exp Bot 57: 887-896
33. Verdonk JC, Haring MA, van Tunen AJ, Schuurink RC (2005) ODORANT1 regulates fragrance biosynthesis in petunia flowers. Plant Cell 17: 1612-1624
34. Wang CC, Cai XZ, Wang XM, Zheng Z (2006) Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis. Funct Plant Biol 33: 347-355
35. Wang K, Ryu C, Kang L, Mysore KS (2007) A high throughput screen using virus-induced gene silencing in Nicotiana benthamiana identifies the requirement of squalene synthase for nonhost resistance. Phytopathology 97: S119-S120
36. Wege S, Scholz A, Gleissberg S, Becker A (2007) Highly efficient virus-induced gene silencing (VIGS) in California poppy (Eschscholzia californica): an evaluation of VIGS as a strategy to obtain functional data from non-model plants. Ann Bot (Lond) 100: 641-649
37. Wiklund S, Johansson E, Sjostrom L, Mellerowicz EJ, Edlund U, Shockcor JP, Gottfries J, Moritz T, Trygg J (2008) Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models. Anal Chem 80: 115-122
38. Xie YH, Zhu BZ, Yang XL, Zhang HX, Fu DQ, Zhu HL, Shao Y, Li YC, Gao HY, Luo YB (2006) Delay of postharvest ripening and senescence of tomato fruit through virus-induced LeACS2 gene silencing. Postharvest Biol Technol 42: 8-15
39. Zhang C, Yang C, Whitham SA, Hill JH (2009) Development and use of an efficient DNA-based viral gene silencing vector for soybean. Mol Plant Microbe Interact 22: 123-131