A novel high-throughput cell-based method for integrated quantification of type I interferons and in vitro screening of immunostimulatory RNA drug delivery

Biotechnology and Bioengineering

Volumn 103, Issue 4, 2009, Pages 664-675

  Nguyen, David N ^a   Kim, Phillip ^b   Martínez Sobrido, Luis ^c,e   Beitzel, Brett ^d   García Sastre, Adolfo ^c   Langer, Robert ^a,b   Anderson, Daniel G ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^d UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

^e UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

Drug delivery; High throughput screening; Immunostimulatory RNA; Innate immunity; RNA interference; siRNA

Indexed keywords

HIGH-THROUGHPUT SCREENING; IMMUNOSTIMULATORY RNA; INNATE IMMUNITY; RNA INTERFERENCE; SIRNA;

ANTIBIOTICS; CELL CULTURE; CELL MEMBRANES; DRUG DELIVERY; DYES; GLYCOPROTEINS; IMMUNOLOGY; INTERFERONS; RNA; THROUGHPUT; TISSUE;

NUCLEIC ACIDS;

ALPHA INTERFERON; BETA INTERFERON; INTERFERON RECEPTOR; NANOPARTICLE; RED FLUORESCENT PROTEIN; RNA;

ARTICLE; CELL ACTIVATION; CONTROLLED STUDY; DRUG DELIVERY SYSTEM; EFFECTOR CELL; ENZYME LINKED IMMUNOSORBENT ASSAY; FUSION GENE; HUMAN; HUMAN CELL; IN VITRO STUDY; INNATE IMMUNITY; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN EXPRESSION; SCREENING TEST;

BIOSENSING TECHNIQUES; CELL LINE; DRUG EVALUATION, PRECLINICAL; GENES, REPORTER; HUMANS; IMMUNOLOGIC FACTORS; INTERFERON TYPE I; LUMINESCENT PROTEINS; RESPONSE ELEMENTS; RNA;

EID: 67650136597     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22312     Document Type: Article

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