Fusion order controls expression level and activity of elastin-like polypeptide fusion proteins

Protein Science

Volumn 18, Issue 7, 2009, Pages 1377-1387

  Christensen, Trine ^a   Amiram, Miriam ^a   Dagher, Sue ^b   Trabbic Carlson, Kimberly ^a   Shamji, Mohammed F ^a,c   Setton, Lori A ^a,d   Chilkoti, Ashutosh ^a  

^a Duke University   (United States)

^b Phase Bioscience Inc   (United States)

^c UNIVERSITY OF OTTAWA   (Canada)

^d DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Elastin like polypeptides; Fusion order; Fusion proteins; Inverse transition cycling; Protein yields; Specific activities

Indexed keywords

ELASTIN; HYBRID PROTEIN; MESSENGER RNA; RECOMBINANT PROTEIN; SYNAPSIN I;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; INVERSE TRANSITION CYCLING; NONHUMAN; PHASE TRANSITION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; RNA TRANSCRIPTION; RNA TRANSLATION;

CENTRIFUGATION; CLONING, MOLECULAR; ELASTIN; ESCHERICHIA COLI; GENE EXPRESSION; INCLUSION BODIES; LUMINESCENT PROTEINS; PEPTIDES; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SODIUM CHLORIDE; TEMPERATURE; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI;

EID: 67650087601     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.157     Document Type: Article

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