High-level Production of Recombinant Arenicola Marina Globin Chains in Escherichia Coli: A New Generation of Blood Substitute

Artificial Cells, Blood Substitutes, and Biotechnology

Volumn 37, Issue 3, 2009, Pages 106-116

  Harnois, Thomas ^a,b   Rousselot, Morgane ^c   Rogniaux, Hlne ^d   Zal, Franck ^a,b  

^a STATION BIOLOGIQUE   (France)

^b CNRS   (France)

^c Hemarina SA   (France)

^d CEMAGREF   (France)

Author keywords

Annelid; Blood substitute; Extracellular haemoglobin; Over expression

Indexed keywords

AMINOLEVULINIC ACID; ANNELID; BI-LAYER; CHROMATOGRAPHIC PURIFICATION; E. COLI; EXTRACELLULAR; EXTRACELLULAR HAEMOGLOBIN; FUSION TAG; GLOBIN CHAINS; HAEMOGLOBINS; HEME SYNTHESIS; HIGH-LEVEL PRODUCTION; OVER-EXPRESSION; OXYGEN BINDING; OXYGEN CARRIER; PURIFICATION PROCEDURES; THERAPEUTIC APPLICATION;

ACIDS; BLOOD; BLOOD SUBSTITUTES; ESCHERICHIA COLI; GENETIC ENGINEERING; MARINAS; OXYGEN; PORPHYRINS; PURIFICATION; SYSTEM THEORY;

HEMOGLOBIN;

AMINOLEVULINIC ACID; BLOOD SUBSTITUTE; GLUTATHIONE TRANSFERASE; HEME; HEMOGLOBIN BETA CHAIN; HEMOGLOBIN BETA2 CHAIN; HYBRID PROTEIN; RECOMBINANT HEMOGLOBIN; UNCLASSIFIED DRUG; GLOBIN; HEMOGLOBIN;

ARENICOLA MARINA; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; HEME SYNTHESIS; HEMOGLOBIN SYNTHESIS; MOLECULAR STABILITY; NONHUMAN; OXYGEN AFFINITY; POLYCHAETA; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; ADULT; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CHEMISTRY; GENETIC ENGINEERING; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; SOLUBILITY; ULTRAVIOLET SPECTROPHOTOMETRY;

ANNELIDA; ARENICOLA MARINA; ESCHERICHIA COLI;

ADULT; AMINO ACID SEQUENCE; AMINOLEVULINIC ACID; ANIMALS; BLOOD SUBSTITUTES; ESCHERICHIA COLI; GENETIC ENGINEERING; GLOBINS; HEME; HEMOGLOBINS; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; POLYCHAETA; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 67649981402     PISSN: 10731199     EISSN: 15324184     Source Type: Journal    
DOI: 10.1080/10731190902908445     Document Type: Article

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