The optimal flow rate and column length for maximum production rate of protein A affinity chromatography

Bioprocess Engineering

Volumn 21, Issue 4, 1999, Pages 287-292

  Fahrner, R L ^a   Iyer, H V ^a   Blank, G S ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MONOCLONAL ANTIBODY; PROTEIN A;

AFFINITY CHROMATOGRAPHY; ANTIBODY PRODUCTION; ARTICLE; BIOPROCESS; MATHEMATICAL ANALYSIS; PRIORITY JOURNAL; PROTEIN PURIFICATION;

EID: 0032859489     PISSN: 0178515X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004490050677     Document Type: Article

References (32)

1. HER2 antibody for human cancer therapy. Proc. Natl. Acad. Sci. USA. 89 (1992) 4285-4289
2. Bodey, B.; Kaiser, H.E.; Goldfarb, R.H.: Immunophenotypically varied cell subpopulations in primary and metastatic human melanomas. Monclonal antibodies for diagnosis, detection of neoplastic progression and receptor directed immunotherapy. Anticancer Res. 16 (1996) 517-532
3. Anderson, D.R.; Grillo-Lopez, A.; Yarns, C.; Chambers, K.S.; Hanna, N.: Targeted anti-cancer therapy using rituximab, a chimeric anti-CD20 antibody (IDEC-C2B8) in the treatment of non-Hodgkin's B-cell lymphoma. Biochem. Soc. Trans. 25 (1996) 705-708
4. Longo, D.L.: Immunotherapy for non-Hodkin's lymphoma. Curr. Opin. Oncol. 8 (1996) 353-359
5. HER2 monoclonal antibody in patients with NER2/neu-overexpressing metastatic breat cancer. J. Clin. Oncol. 14(3) (1996) 737-744
6. Kagel, R.A.; Kagel, G.W.; Garg, V.K.: High performance protein A- and immuno-affinity chromatography for the commercial purification of anti-human tissue plasminogen activator. BioChromatography. 4(5) (1989) 246-252
7. Hjelm, H.; Hjelm, K.; Sjoquist, J.: Protein A from Staphyococcus aureus. Its isolation by affinity chromatography and its use as an aimmunosorbent for isolation of immunoglobulins. FEBS Lett. 28(1) (1972) 73-76
8. Leser, E.W.; Asenjo, J.A.: Rational design of purification processes for recombinant proteins. J. Chromatogr. 584 (1992) 43-57
9. Surolia, A.; Pain, D.; Khan, M.I.; Protein A: Nature's universal anti-antibody. Trends. Biochem. Sci. 7 (1982) 74-76
10. Ey, P.L.; Prowse, S.J.; Jenkin, C.R.: Isolation of pure IgG1,IgG2a, and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry. 15 (1978) 429-436
11. Reis, K.J.; Boyle, M.D.P.; Ayoub, E.M.: Identification of distinct Fc-receptor molecules on Steptococci and Staphylococci. J. Clin. Lab. Immunol. 13 (1984) 75-80
12. Lindmark, R.; Thoren-Tolling, K.; Sjoquist, J.: Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera. J. Immunol. Meth. 62 (1983) 1-13
13. Kamiya, Y.; Majima, T.; Sohma, Y.; Katoh, S.; Sada, E.: Effective purification of bioproducts by fast flow affinity chromatography. J. Ferment. Bioeng. 69(5) (1990) 298-301
14. Tu, Y.Y.; Primus, F.J.; Goldenberg, D.M.: Temperature affects binding of murine monoclonal IgG antibodies to protein A. 109 (1988) 43-47
15. Van Sommeren, A.P.G.; Machielsen, P.A.G.M.; Gribnau, T.C.J.: Effects of temperature, flow rate and composition of binding buffer on adsorption of mouse monoclonal IgG1 antibodies to protein A Sepharose Fast Flow. Prep. Biochem. 22(2) (1992) 135-149
16. Katoh, S.; Kambayashi, T.; Deguchi, R.; Yoshida, F.: Performance of affinity chromatography columns. Biotechnol. Bioeng. XX (1978) 267-280
17. Schuler, G.; Reinacher, M.: Development and optimization of a single-step procedure using protein A affinity chromatography to isolate murine IgG1 monoclonal antibodies from hybridoma supernatants. J. Chromatogr. 587 (1991) 61-70
18. Fuglistaller, P.: Comparison of immunoglobulin binding capacities and ligand leakage using eight different protein A affinity chromatography matricies. J. Immunol. Methods. 124 (1989) 171-177
19. Mao, Q.M.; Prince, I.G; Hearn, M.T.W.: High-performnace liquid chromatography of amino acids, peptides, and proteins CXXXIX: impact of operating parameters in large-scale chromatography of proteins. J. Chromatogr. A. 691 (1995) 273-283
20. Jandera, P.; Komers, D.; Guichon, G.: Effects of the gradient profile on the production rate in reversed-phase gradient elution overload chromatography. J. Chromatogr. A. 760 (1997) 25-39
21. Mao, Q.M.; Prince, I.G.; Hearn, M.T.W.: High-performance liquid chromatography of amino acids, peptides and proteins CXXXII: optimization of operating parameters for protein purification with Chromatographic columns. J. Chromatogr. 646 (1993) 81-89
22. Felinger, A.; Guiochon, G.: Optimization of the experimental conditions and the column design parameters in overloaded elution chromatography. J. Chromatogr. 591 (1992) 31-45
23. Katoh, S.: Scaling-up affinity chromatography. Trends Biotechnol. 5 (1987) 328-331
24. Horstmann, B.J.; Chase, H.A.: Modelling the affinity adsorption of immunoglobulin G to protein A immobilized to agarose matricies. Chem. Eng. Res. Des. 67 (1989) 243-254
25. Yamamoto, S.; Sano, Y.: Short-cut method for predicting the productivity of affinity chromatography. J. Chromatogr. 597 (1992) 173-179
26. van Reis, R.; Leonard, L.C.; Hsu, C.C.; Builder, S.E.: Industrial scale harvest of proteins from mammalian cell culture by tangential flow filtration. Biotechnol. Bioeng. 38 (1991) 413-422
27. Nugent, K.; Olson, K.: Applications of ultrafast protein analysis for real-time protein process monitoring. BioChromatography. 5(2) (1990) 101-105
28. Olson, K.C.; Gehant, R.L.: Applications of ultrafast HPLC to process development of recombinant DNA-derived proteins. Biotechnol. Prog. 8 (1992) 562-566
29. Ozturk, S.S.; Thrift, J.C.; Blackie, J.D.; Naveh, D.: Real-time monitoring of protein secretion in mammalian cell fermentation: measurement of monoclonal antobodies using a computer-controlled HPLC system (BioCad/RPM). Biotechnol. Bioeng. 48 (1995) 201-206
30. Afeyan, N.B.; Fulton, S.P.; Gordon, N.F.; Mazsaroff, I.; Varaday, L.; Regnier, F.E.: Perfusion chromatography: an approach to purifying biomolecules. Bio/Technology. 8 (1990) 203-206
31. Fulton, S.P.; Afeyan, N.B.; Gordon, N.F.; Regnier, F.E.: Very high speed separation of proteins with a 20-um reversed-phase sorbent. J. Chromatogr. 547 (1991) 452-456
32. Paliwal, S.K.; Nadler, T.K.; Wang, D.I.C.; Regnier, F.E.: Automated process monitoring of monclonal antibody production. Anal. Chem. 65 (1993) 3363-3367