Germline P granules are liquid droplets that localize by controlled dissolution/condensation

Science

Volumn 324, Issue 5935, 2009, Pages 1729-1732

  Brangwynne, Clifford P ^a,b,c   Eckmann, Christian R ^a   Courson, David S ^c   Rybarska, Agata ^a   Hoege, Carsten ^a   Gharakhani, Jöbin ^b,c   Jülicher, Frank ^b,c   Hyman, Anthony A ^a,c  

^a MAX PLANCK INSTITUTE OF MOLECULAR CELL BIOLOGY AND GENETICS   (Germany)

^b MAX PLANCK INSTITUT FÜR PHYSIK KOMPLEXER SYSTEME   (Germany)

^c MARINE BIOLOGICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONDENSATION; DISSOLUTION; DROPLET; NEMATODE; PHYSICOCHEMICAL PROPERTY; SEXUAL REPRODUCTION;

ARTICLE; CAENORHABDITIS ELEGANS; DISSOLUTION; GERM LINE; LIQUID; NONHUMAN; PHASE TRANSITION; POLYMERIZATION; PRIORITY JOURNAL; SURFACE TENSION; VISCOSITY; ANIMAL; ANIMAL EMBRYO; CELL GRANULE; CHEMISTRY; CYTOLOGY; CYTOPLASM; GERM CELL; METABOLISM; PHYSICAL CHEMISTRY; PHYSIOLOGY; PRENATAL DEVELOPMENT; RNA INTERFERENCE; SOLUBILITY; ULTRASTRUCTURE;

CAENORHABDITIS ELEGANS;

CAENORHABDITIS ELEGANS PROTEIN; HELMINTH RNA; MEX 5 PROTEIN, C ELEGANS; MEX-5 PROTEIN, C ELEGANS; PAR 1 PROTEIN, C ELEGANS; PAR-1 PROTEIN, C ELEGANS; PROTEIN SERINE THREONINE KINASE;

ANIMALS; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CYTOPLASM; CYTOPLASMIC GRANULES; EMBRYO, NONMAMMALIAN; GERM CELLS; PHASE TRANSITION; PHYSICOCHEMICAL PROCESSES; PROTEIN-SERINE-THREONINE KINASES; RNA INTERFERENCE; RNA, HELMINTH; SOLUBILITY; SURFACE TENSION; VISCOSITY;

EID: 67649976463     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1172046     Document Type: Article

References (23)

1. G. Seydoux, R. E. Braun, Cell 127, 891 (2006).
2. S. Strome, R. Lehmann, Science 316, 392 (2007).
3. S. O. Schneider, B. Bowerman, Annu. Rev. Genet. 37, 221 (2003).
4. S. N. Hird, J. G. White, J. Cell Biol. 121, 1343 (1993).
5. S. N. Hird, J. E. Paulsen, S. Strome, Development 122, 1303 (1996).
6. R. J. Cheeks et al., Curr. Biol. 14, 851 (2004).
7. C. DeRenzo, K. J. Reese, G. Seydoux, Nature 424, 685 (2003).
8. C. A. Spike, S. Strome, Curr. Biol. 13, R837 (2003).
9. See supporting material on Science Online.
10. I. Kawasaki et al., Cell 94, 635 (1998).
11. M. E. Gruidl et al., Proc. Natl. Acad. Sei. U.S.A. 93, 13837 (1996).
12. D. R. Hamill, A. F. Severson, J. C. Carter, B. Bowerman, Dev. Cell 3, 673 (2002).
13. J. R. Tenlen, J. N. Molk, N. London, B. D. Page, J. R. Priess, Development 135, 3665 (2008).
14. A. A. Cuenca, A. Schetter, D. Aceto, K. Kemphues, G. Seydoux, Development 130, 1255 (2003).
15. C. Schubert, R. Lin, C. de Vries, R. Plasterk, J. Priess, Mol. Cell 5, 671 (2000).
16. B. M. Lunde, C. Moore, G. Varani, Nat Rev. Mol. Cell Biol. 8, 479 (2007).
17. K. E. Kasza et al., Curr. Opin. Cell Biol. 19, 101 (2007).
18. M. J. Strasser et al., BMC Dev. Biol. 8, 58 (2008).
19. D. G. Aarts, M. Schmidt, H. N. Lekkerkerker, Science 304, 847 (2004).
20. M. Daoud, C. E. Williams, S. N. Lyle, Eds., Soft Matter Physics (Springer, Berlin, 1999).
21. J. D. Gunton, M. S. Miguel, P. S. Sahni, in Phase Transitions and Critical Phenomena, C. Domb, M. S. Green, Eds. (Academic Press, London, 1983), vol. 8.
22. P. Anderson, N. Kedersha, J. Cell Biol. 172, 803 (2006).
23. We thank T. Mitchison for advice and discussion, together with students of the MBL Physiology Course 2008; and S. Grill, N. Goehring, and members of the Hyman and Eckmann Laboratories for helpful comments. We thank S. Hell, R. Medda, and B. Harke for assistance with stimulated emission depletion microscopy. Some strains used in this study were obtained from the Caenorhabditis Genetics Center. C.P.B. is supported by a fellowship from the Helen Hay Whitney Foundation.