Segregation of germ granules in living Caenorhabditis elegans embryos: Cell-type-specific mechanisms for cytoplasmic localisation

Development

Volumn 122, Issue 4, 1996, Pages 1303-1312

  Hird, Steven N ^a,c   Paulsen, Janet E ^b,d   Strome, Susan ^b  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b INDIANA UNIVERSITY   (United States)

^c UNIVERSITY OF COLORADO   (United States)

^d PFIZER INC   (United States)

Author keywords

Caenorhabditis elegans; Cytoplasmic localisation; Germ granule; In vivo confocal fluorescence microscopy; P granules

Indexed keywords

GENE PRODUCT; RIBONUCLEOPROTEIN;

ARTICLE; CAENORHABDITIS ELEGANS; CELL TYPE; CENTROSOME; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CYTOPLASM; EMBRYO; EMBRYO DEVELOPMENT; EMBRYO SEGMENTATION; GERM CELL; GERM GRANULE; INTRACELLULAR TRANSPORT; MICROFILAMENT; MICROTUBULE; NONHUMAN; P GRANULE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN TRANSPORT; SOMATIC CELL;

ANIMALS; CAENORHABDITIS ELEGANS; CELL NUCLEUS; CYTOPLASM; CYTOPLASMIC GRANULES; GENES, HELMINTH; GERM CELLS; HELMINTH PROTEINS; HERMAPHRODITISM; MICROFILAMENTS; MICROTUBULES; MUTATION; RIBONUCLEOPROTEINS;

EID: 0029880495     PISSN: 09501991     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. Albertson, D. G. (1984). Formation of the first cleavage spindle in nematode embryos. Dev. Biol. 101, 61-72.
2. Bowerman, B., Draper, B. W., Mello, C. C. and Priess, J. R. (1993). The maternal gene skn-1 encodes a protein that is distributed unequally in early C. elegans embryos. Cell 74, 443-452.
3. Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71-94.
4. Capowski, E. E., Martin, P., Garvin, C. and Strome, S. (1991). Identification of grandchildless loci whose products are required for normal germ-line development in the nematode C. elegans. Genetics 129, 1061-1072.
5. Cheng, N. N., Kirby, C. M. and Kemphues, K. J. (1995). Control of cleavage spindle orientation in Caenorhabditis elegans: the role of the genes par-2 and par-3. Genetics 139, 549-559.
6. Deppe, U., Schierenberg, E., Cole., T., Kreig, C., Schmitt, D., Yoder, B. and von Ehrenstein, G. (1978). Cell lineages of the embryo of the nematode Caenorhabditis elegans. Proc Natl. Acad. Sci. USA 75, 376-380.
7. Edgar, L. G. and McGhee, J. D. (1986). Embryonic expression of a gut-specific esterase in Caenorhabditis elegans. Dev. Biol. 114, 109-118.
8. Edgar, L. G. and Wood, W. B. (1993). In Essential Developmental Biology: A Practical Approach (ed. C. D. Stern & P. W. H. Holland), pp. 11-20. Oxford: IRL. Oxford University Press.
9. Etemad-Moghadam, B., Guo, S., and Kemphues, K.J. (1995). Asymmetrically distributed PAR-3 protein contributes to the cell polarity and spindle alignment in early C. elegans embryos. Cell 83, 743-752.
10. Fire, A. (1986). Integrative transformation of C. elegans. EMBO J 5, 2673-2680.
11. Goding, J. W. (1976). Conjugation of antibodies with fluorochromes: modifications to the standard methods. J. Immunol. Meth. 13, 215-226.
12. Guo, S. and Kemphues, K. J. (1995) par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell 81, 611-620.
13. Hill, D. P. and Strome, S. (1988). An analysis of the role of microfilaments in the establishment and maintenance of asymmetry in Caenorhabditis elegans zygotes. Dev. Biol. 125, 75-84.
14. Hill, D. P. and Strome, S. (1990). Brief cytochalasin-induced disruption of microfilaments during a critical interval in one-cell C. elegans embryos alters the partitioning of developmental instructions to the two-cell embryo. Development 108, 159-172.
15. Hird, S. N. and White, J. G. (1993). Cortical and cytoplasmic flow polarity in early embryonic cells of Caenorhabditis elegans. J. Cell Biol. 121, 1343-1355.
16. Hirsh, D., Oppenheim, D. and Klass, M. (1976). Development of the reproductive system of Caenorhabditis elegans. Dev. Biol. 49, 200-219
17. Hudson, L. and Hay, F.C. (1980). Practical Immunology. Oxford, UK: Blackwell Scientific Publications.
18. Hyman, A. A. (1989). Centrosome movement in the early divisions of C. elegans. A cortical site determining centrosome position. J. Cell Biol. 109, 1185-1193.
19. Hyman, A. A. and White, J.G.(1987). Determination of cell division axes in the early embryogenesis of Caenorhabditis elegans. J. Cell Biol. 105, 2123-2135.
20. Kemphues, K. J., Priess, J. R., Morton, D. G. and Cheng, N. S. (1988). Identification of genes required for cytoplasmic localization in early C. elegans embryos. Cell 52, 311-320.
21. Kirby, C., Kusch, M. and Kemphues, K. (1990). Mutations in the par genes of Caenorhabditis elegans affect cytoplasmic reorganisation during the first cell cycle. Dev. Biol. 142, 203-215.
22. Kreis, T. E. and Birchmeier, W. (1980). Stress fibre sarcomeres of fibroblasts are contractile. Cell 22, 555-561.
23. Lehmann, R. (1992). Germ-plasm formation and germ-cell determination in Drosophila. Curr. Op. Gen. Dev. 2, 543-549.
24. Levitan, D. J., Boyd, L., Mello, C. C., Kemphues, K. J. and Stinchcomb, D. (1994). par-2, a gene required for blastomere asymmetry in C. elegans, encodes zinc-finger and ATP-binding motifs. Proc. Natl. Acad. Sci USA 91, 6108-6112.
25. Morton, D. G., Roos, J. M. and Kemphues, K. J. (1992). par-4, a gene required for cytoplasmic localization and determination of specific cell types in Caenorhabditis elegans embryogenesis. Genetics 130, 771-790.
26. Nigon, V., Guerrier, P. and Monin, H. (1960). L'architecture polaire de l'oeuf et les movements des constituants cellulaires au cours des premieres etapes du developpement chez quelques nematodes. Bull Biol. Fr. Belg. 94, 131-202.
27. Rose, L. S., Lamb, M., Hird, S. N. and Kemphues, K. J. (1995). Pseudocleavage is dispensable for polarity and development in Caenorhabditis elegans. Dev. Biol. 168, 479-489.
28. Schierenberg, E. (1987). Reversal of cellular polarity and early cell-cell interactions in the embryo of Caenorhabditis elegans. Dev. Biol. 122, 452-463.
29. Seydoux, G. and Fire, A. (1994). Soma-germline asymmetry in the distribution of embryonic RNAs in C. elegans. Development 120, 2317-2328.
30. Skiba, F. and Schierenberg, E. (1992), Cell lineages, developmental timing, and spatial pattern formation in embryos of free-living soil nematodes. Dev. Biol. 151, 597-610.
31. Strome, S. (1986) Establishment of asymmetry in early Caenorhabditis elegans embryos: visualisation with antibodies to germ cell components In Gametogenesis and the Early Embryo (ed. J. G. Gall), pp. 77-95 New York: Alan R. Liss
32. Strome, S., Martin, P., Schierenberg, E. and Paulsen, J. (1995). Transformation of the germ line into muscle in mes-1 mutant embryos of C. elegans. Development 121, 2961-2972.
33. Strome, S. and Wood, W. B. (1982). Immunofluorescence visualisation of germ-line specific cytoplasmic granules in embryos, larvae, and adults of Caenorhabditis elegans. Proc. Natl. Acad. Sci USA 79, 1558-1562
34. Strome, S. and Wood, W. B. (1983). Generation of asymmetry and segregation of germline granules in early C. elegans embryos. Cell 35, 15-25
35. Sulston, J. E., Schierenberg, E., White, J. G. and Thomson, J. N. (1983). The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. 100, 64-119.
36. The, T. H. and Feltkamp, T. E. W. (1969). Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. J. Immunology 18, 865-873.
37. Wolf, N., Priess, J. R. and Hirsh, D. (1983). Segregation of germline granules in early embryos of C. elegans: an electron microscopic analysis. J. Embryol Exp. Morph 73, 297-306.
38. Wood, W.B., Schierenberg, E., and Strome, S. (1984) Localisation and determination in early embryos of Caenorhabditis elegans. In Molecular Biology of Development (ed. E. H. Davidson & R. A. Firtel). pp. 37-49 New York: Alan R. Liss.