Shotgun proteomic analysis of the microsomal fraction of eukaryotic cells using a two-dimensional reversed-phase x ion-pair reversed-phase HPLC setup

Journal of Separation Science

Volumn 32, Issue 8, 2009, Pages 1165-1174

  Wörner, Martin ^a   Melchior, Katja ^a   Delmotte, Nathanaël ^a   Hwang, Kyung Hoon ^a,b   Monostory, Katalin ^c   Huber, Christian G ^a,d   Bernhardt, Rita ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b KIST   (Germany)

^c CHEMICAL RESEARCH CENTER   (Hungary)

^d UNIVERSITY OF SALZBURG   (Austria)

Author keywords

Microsomal proteins; Primary human hepatocytes; Shotgun proteomics; Subfractionation

Indexed keywords

CHOLESTEROL HOMEOSTASIS; EUKARYOTIC CELLS; EXPERIMENTAL SETUP; FISSION YEAST; HEPATOCYTE; ION PAIRS; MEMBRANE FRACTION; MEMBRANE PROTEINS; MICROSOMAL PROTEINS; PRIMARY HUMAN HEPATOCYTES; PROTEOMES; PROTEOMIC ANALYSIS; PROTEOMIC APPROACHES; PROTEOMIC STUDIES; REPRODUCIBILITY; REVERSED PHASE; REVERSED PHASE HPLC; RP-HPLC; SAMPLE LOADS; SCHIZOSACCHAROMYCES POMBE; SHOTGUN PROTEOMICS; SUB-CELLULAR; SUBFRACTIONATION; TANDEM MS;

BODY FLUIDS; CHOLESTEROL; DETOXIFICATION; GELATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR BIOLOGY; PHYSIOLOGY; PINCH EFFECT;

PROTEINS;

CELL MEMBRANE PROTEIN; CHOLESTEROL; LIPID; PROTEOME; SCHIZOSACCHAROMYCES POMBE PROTEIN;

ARTICLE; CELL COMPARTMENTALIZATION; CHOLESTEROL METABOLISM; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; EUKARYOTIC CELL; EXPERIMENTAL DESIGN; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; ION PAIR CHROMATOGRAPHY; LIPID METABOLISM; LIVER MICROSOME; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; REPRODUCIBILITY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SCHIZOSACCHAROMYCES POMBE; TANDEM MASS SPECTROMETRY; XENOBIOTIC METABOLISM; BIOLOGY; CELL CULTURE; CHEMISTRY; CYTOLOGY; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSTRUMENTATION; LIVER CELL; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MICROSOME; PROTEOMICS; SCHIZOSACCHAROMYCES;

EUKARYOTA; SCHIZOSACCHAROMYCES POMBE; SCHIZOSACCHAROMYCETACEAE;

CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COMPUTATIONAL BIOLOGY; EUKARYOTIC CELLS; HEPATOCYTES; HUMANS; MASS SPECTROMETRY; MICROSOMES; PROTEOME; PROTEOMICS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

EID: 67649723078     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.200800619     Document Type: Article

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