Experimental approach for an in vitro toxicity assay with non-aggregated quantum dots

Toxicology in Vitro

Volumn 23, Issue 5, 2009, Pages 955-962

  Koeneman, Brian A ^a   Zhang, Yang ^b   Hristovski, Kiril ^b   Westerhoff, Paul ^b   Chen, Yongsheng ^b   Crittenden, John C ^b,c   Capco, David G ^a  

^a ARIZONA STATE UNIVERSITY   (United States)

^b AZ 85281   (United States)

^c Georgia Institute of Technology   (United States)

Author keywords

Aggregation; Nanomaterials; Quantum dots; Toxicity

Indexed keywords

CADMIUM; CALCIUM; MAGNESIUM; NANOMATERIAL; NANOPARTICLE; PHOSPHATE BUFFERED SALINE; QUANTUM DOT; THIOGLYCOLIC ACID;

ARTICLE; CELL DEATH; CELL STRAIN CACO 2; ELECTRIC RESISTANCE; HEALTH HAZARD; HYDROPHILICITY; IN VITRO STUDY; INTESTINE EPITHELIUM; MATERIAL COATING; PARTICLE SIZE; TOXICITY TESTING;

CACO-2 CELLS; CADMIUM COMPOUNDS; CELL DEATH; ELECTRIC IMPEDANCE; HUMANS; LIGANDS; PARTICLE SIZE; QUANTUM DOTS; TELLURIUM; THIOGLYCOLATES; TOXICITY TESTS;

EID: 67649576961     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2009.05.007     Document Type: Article

References (24)

1. Choi A.O., Brown S.E., Szyf M., and Maysinger D. Quantum dot-induced epigenetic and genotoxic changes in human breast cancer cells. J. Mol. Med. 86 (2008) 291-302
2. Clescerl, L.S., Greenberg, A.E., Eaton, A.D., 1998. Standard Methods for the Examination of Water and Wastewater, twentieth ed. American Public Health Association, Washington, DC.
3. Cowin P., Kapprell H.P., Franke W.W., Tamkun J., and Hynes R.O. Plakoglobin - a protein common to different kinds of intercellular adhering junctions. Cell 46 (1986) 1063-1073
4. Cui D., Tian F., Ozkan C.S., Wang M., and Gao H. Effect of single wall carbon nanotubes on human HEK293 cells. Toxicol. Lett. 155 (2005) 73-85
5. Fossati L., Dechaume R., Hardillier E., Chevillon D., Prevost C., Bolze S., and Maubon N. Use of simulated intestinal fluid for Caco-2 permeability assay of lipophilic drugs. Int. J. Pharm. 360 (2008) 148-155
6. Griffitt R.J. Exposure to copper nanoparticles causes injury and acute lethality in zebrafish (Danio rerio). Environ. Sci. Technol. 41 (2007) 8178-8186
7. Henry T.B., Menn F.M., Fleming J.T., Wilgus J., Compton R.N., and Sayler G.S. Attributing effects of aqueous C60 nano-aggregates to tetrahydrofuran decomposition products in larval zebrafish by assessment of gene expression. Environ. Health Perspect. 115 (2007) 1059-1065
8. Koeneman B.A., Lee K.K., Singh A., He J., Raupp G.B., Panitch A., and Capco D.G. An ex vivo method for evaluating the biocompatibility of neural electrodes in rat brain slice cultures. J. Neurosci. Methods 137 (2004) 257-263
9. Lind M.L., Jacobsen J., Holm R., and Mullertz A. Development of simulated intestinal fluids containing nutrients as transport media in the Caco-2 cell culture model: assessment of cell viability, monolayer integrity and transport of a poorly aqueous soluble drug and a substrate of efflux mechanisms. Eur. J. Pharm. Sci. 32 (2007) 261-270
10. Liu Z.W., and Chen B.H. Copper treatment alters the barrier functions of human intestinal Caco-2 cells: involving tight junctions and P-glycoprotein. Human Exp. Toxicol. 23 (2004) 369-377
11. Maynard A.D., et al. Safe handling of nanotechnology. Nature 444 (2006) 267-269
12. Monteiro-Riviere N.A., Nemanich R.J., Inman A.O., Wang Y.Y., and Riviere J.E. Multi-walled carbon nanotube interactions with human epidermal keratinocytes. Toxicol. Lett. 155 (2005) 377-384
13. Nel A., Xia T., Madler L., and Li N. Toxic potential of materials at the nanolevel. Science 311 (2006) 622-627
14. Oberdörster, G. et al., 2005. Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Part. Fibre Toxicol. 2 (8).
15. Peterson M.D., and Mooseker M.S. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. J. Cell Sci. 102 Pt 3 (1992) 581-600
16. Peterson M.D., and Mooseker M.S. An in vitro model for the analysis of intestinal brush border assembly. I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells. J Cell Sci. 105 Pt 2 (1993) 445-460
17. Peterson M.D., Bement W.M., and Mooseker M.S. An in vitro model for the analysis of intestinal brush border assembly. II. Changes in expression and localization of brush border proteins during cell contact-induced brush border assembly in Caco-2BBe cells. J. Cell Sci. 105 Pt 2 (1993) 461-472
18. Stearns R.C., Paulauskis J.D., and Godleski J.J. Endocytosis of ultrafine particles by A549 cells. Am. J. Respir. Cell Mol. Biol. 24 (2001) 108-115
19. Stern S.T., Zolnik B.S., McLeland C.B., Clogston J., Zheng J., and McNeil S.E. Induction of autophagy in porcine kidney cells by quantum dots: a common cellular response to nanomaterials?. Toxicol. Sci. 106 (2008) 140-152
20. Tang M., et al. Unmodified CdSe quantum dots induce elevation of cytoplasmic calcium levels and impairment of functional properties of sodium channels in rat primary cultured hippocampal neurons. Environ. Health Perspect. 116 (2008) 915-922
21. Woodrow Wilson International Center for Scholars (WWICS), (2008). The Project on Emerging Nanotechnologies, Available from (Retrieved October 16, 2008).
22. Yacobi N.R., Phuleria H.C., Demaio L., Liang C.H., Peng C.A., Sioutas C., Borok Z., Kim K.J., and Crandall E.D. Nanoparticle effects on rat alveolar epithelial cell monolayer barrier properties. Toxicol. In Vitro 21 (2007) 1373-1381
23. Zhang Y., Chen Y., Westerhoff P., Hristovski K., and Crittenden J.C. Stability of commercial metal oxide nanoparticles in water. Water Res. 42 (2008) 2204-2212
24. Zhang Y., Chen Y.S., Westerhoff P., and Crittenden J.C. Stability and removal of water soluble CdTe quantum dots in water. Environ. Sci. Technol. 42 (2008) 321-325