dUTP incorporation into genomic DNA is linked to transcription in yeast

Nature

Volumn 459, Issue 7250, 2009, Pages 1150-1153

  Kim, Nayun ^a   Jinks Robertson, Sue ^a  

^a DUKE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APURINIC ACID; DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; GENOMIC DNA; THYMIDINE TRIPHOSPHATE; URACIL; DEOXYURIDINE PHOSPHATE; DEOXYURIDINE TRIPHOSPHATE; FUNGAL DNA;

COMPARTMENTALIZATION; DNA; GENE EXPRESSION; GENOME; MUTATION; RECOMBINATION; RNA; YEAST;

APYRIMIDINIC SITE; ARTICLE; CELL COMPARTMENTALIZATION; CONTROLLED STUDY; DNA STRUCTURE; DNA SYNTHESIS; DNA TRANSCRIPTION; NUCLEOTIDE METABOLISM; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; YEAST; BIOLOGICAL MODEL; FUNGAL GENOME; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; MUTATION; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; SACCHAROMYCES;

DEOXYURACIL NUCLEOTIDES; DNA, FUNGAL; GENOME, FUNGAL; MODELS, GENETIC; MUTAGENESIS, INSERTIONAL; MUTATION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION, GENETIC;

EID: 67649559461     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature08033     Document Type: Article

References (15)

1. Aguilera, A. The connection between transcription and genomic instability. EMBO J. 21, 195 201 (2002).
2. Kim, N., Abdulovic, A. L., Goay, R., Lippert, M. J. & Jinks-Robortson, S. Transcription-associated mutagenesis in yeast is directly proportiona to the; leve of gone expression and influenced by the direction of DNA replication. DNA Repair (Amst.) 6, 1285-1296 (2007).
3. Lippert, M. J., Freedman, J. A., Barber, M. A. & Jinks-Robertson, S. Identification of a distinctive mutation spectrum associated with high levels of transcription in yeast, Mol. Cell. Biol. 24, 4801-4809 (2004).
4. Harfe, B. D. & Jinks-Robertson, S. Removal of frameshift intermediates by mismatch repair proteins in Saccharomyccs cerevisiae. Mol. Cell. Biol. 19, 4766-4773 (1999).
5. Harfe, B. D. & Jinks-Robertson, S. DNA plymerase ζ introduces multiple mutations when bypassing spontaneous DNA damage in Saccharomyces cerevisiae. Mol. Cell 6, 1491-1499 (2000).
6. Minesinger, B. K. & Jinks-Robertson, S. Roles of RAD6 epistasis group members in spontaneous Polζ-dependent translesion synthesis in Saccharomyces cerevisiae. Genetics 169, 1939 1955 (2005).
7. Boiteux, S. & Guillet, M. Abasic sites in DNA: repair and biological consequences in Saccharomyces cerevisiae. DNA Repair (Amst.) 3, 1-12 (2004).
8. Popoff, S. C., Spira, A. S., Johnson, A. W. & Demple, B. The yeast structural gene (APN1) for the major apurinic endonuclease:homology to E. coli endonucelase IV. Proc. Natl Acad. Sci. USA 87, 4193-4197 (1985).
9. Senturker, S. et al. Substrate specificities of Ntg1 and Ntg2 proteins of Saccharomyces cerevisiae for oxidized DNA bases are not identical. Nucleic Acids Res. 26, 5270-5276 (1998).
10. Swanson, R. L., Morey, N. J., Doetsch, P. W. & Jinks-Robertson, S. Ovelapping specificities of base excision repair, nucleotide excision repair, recombination, and translesion synthesis pathways for DNA base damage in Saccharomyces cerevisiae. Mol. Cell. Biol. 19, 2929-2935 (1999).
11. You, H. J. et al. Saccharomyces cerevisiae Ntg1p and Ntg2p: broad specificity N-glycosylases for the repair of oxidative DNA damage in the nucleus and mitochondria, Biochemistry 38, 11298-11306 (1999).
12. Gadsden, M. H., Mcintosh, E. M., Game, J. C., Wilson, P. J. & Haynes, R. H. dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae. EMBO J. 12, 4425-4431 (1993).
13. Guillet, M., Van Der Kemp, P. A. & Boiteux, S. dUTPase activity is crtical to maintain genetic stability in Saccharomyces cerevisiae. Nucleic Acids Res. 34, 2056-2066 (2006).
14. Christianson, T. W., Sikorski, R. S., Dante, M., Shero, J. H. & Hieter, P. Multifunctional yeast high-copy-number shuttle vectors. Gene 110, 119-122 (1992).
15. Spell, R. M. & Jinks-Robertson, S. in Genetic Recombination: Reviews and Protocols Vol, 262 (ed, Waldman, A. S.) 3-12 (Humana, 2004).