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67649479501
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Protein Phosphorylation and Signaling
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Kemp, B.E.1
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26
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67649503108
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lI complex of methylated compound 2 upon addition of pyrophosphate anion. Titration of the complex with pyrophosphate gave the Kd value of 53 μM.
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lI complex of methylated compound 2 upon addition of pyrophosphate anion. Titration of the complex with pyrophosphate gave the Kd value of 53 μM.
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27
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67649514977
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The addition of more than three equivalents of ATP to the peptide sensor 1 induced an excitation change at two wavelengtth (360 and 410 nm), which indicates the coordination of ATP to the metal complex moiety.
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The addition of more than three equivalents of ATP to the peptide sensor 1 induced an excitation change at two wavelengtth (360 and 410 nm), which indicates the coordination of ATP to the metal complex moiety.
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28
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0022628244
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0024353865
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Walsh, D.A.5
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67649506444
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18 reverse-phase HPLC (highperformance liquid chromatography). Time-dependent production of phosphorylated product was observed when the sensor 1 was exposed to PKA catalytic subunit. The phosphorylated product co-eluted with authentic standard 1P from the HPLC column.
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18 reverse-phase HPLC (highperformance liquid chromatography). Time-dependent production of phosphorylated product was observed when the sensor 1 was exposed to PKA catalytic subunit. The phosphorylated product co-eluted with authentic standard 1P from the HPLC column.
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