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Volumn 11, Issue 13, 2009, Pages 2732-2735

Anion sensor-based ratiometric peptide probe for protein kinase activity

Author keywords

[No Author keywords available]

Indexed keywords

ANION; CADMIUM; COUMARIN DERIVATIVE; CYCLIC AMP DEPENDENT PROTEIN KINASE; FLUORESCENT DYE; PEPTIDE; WATER;

EID: 67649458218     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol9006508     Document Type: Article
Times cited : (31)

References (30)
  • 1
    • 67649479501 scopus 로고    scopus 로고
    • Protein Phosphorylation and Signaling
    • Chem. Rev. 2001, 101, issue 8: Protein Phosphorylation and Signaling.
    • (2001) Chem. Rev , vol.101 , Issue.8
  • 5
    • 0035904886 scopus 로고    scopus 로고
    • Hofmann, R. M.; Cotton, G. J.; Chang, E. J.; Vidal, E.; Veach, D.; Bornmann, W.; Muir, T. W. Bioorg. Med. Chem. Lett. 2001, 11, 30913094.
    • (b) Hofmann, R. M.; Cotton, G. J.; Chang, E. J.; Vidal, E.; Veach, D.; Bornmann, W.; Muir, T. W. Bioorg. Med. Chem. Lett. 2001, 11, 30913094.
  • 25
  • 26
    • 67649503108 scopus 로고    scopus 로고
    • lI complex of methylated compound 2 upon addition of pyrophosphate anion. Titration of the complex with pyrophosphate gave the Kd value of 53 μM.
    • lI complex of methylated compound 2 upon addition of pyrophosphate anion. Titration of the complex with pyrophosphate gave the Kd value of 53 μM.
  • 27
    • 67649514977 scopus 로고    scopus 로고
    • The addition of more than three equivalents of ATP to the peptide sensor 1 induced an excitation change at two wavelengtth (360 and 410 nm), which indicates the coordination of ATP to the metal complex moiety.
    • The addition of more than three equivalents of ATP to the peptide sensor 1 induced an excitation change at two wavelengtth (360 and 410 nm), which indicates the coordination of ATP to the metal complex moiety.
  • 30
    • 67649506444 scopus 로고    scopus 로고
    • 18 reverse-phase HPLC (highperformance liquid chromatography). Time-dependent production of phosphorylated product was observed when the sensor 1 was exposed to PKA catalytic subunit. The phosphorylated product co-eluted with authentic standard 1P from the HPLC column.
    • 18 reverse-phase HPLC (highperformance liquid chromatography). Time-dependent production of phosphorylated product was observed when the sensor 1 was exposed to PKA catalytic subunit. The phosphorylated product co-eluted with authentic standard 1P from the HPLC column.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.