A completely in vitro system for obtaining scFv using mRNA display, PCR, direct sequencing, and wheat embryo cell-free translation

Biotechnology Letters

Volumn 31, Issue 7, 2009, Pages 1103-1110

  Shibui, Tatsuro ^a   Kobayashi, Teruaki ^a   Kanatani, Keiichiro ^a  

^a MITSUBISHI CHEMICAL CORPORATION   (Japan)

Author keywords

Antibody; Direct sequencing; mRNA display; ScFv; Wheat embryo cell free translation

Indexed keywords

AMPLIFIED FRAGMENTS; BATCH METHODS; CELL-FREE SYSTEM; DIRECT SEQUENCING; EMBRYO CELLS; IN-VITRO; MRNA DISPLAY; REACTION MIXTURE; SCFV; SOLUBLE PROTEINS; WHEAT EMBRYO CELL-FREE TRANSLATION;

ANTIBODIES; CLONING; DIALYSIS;

GRAIN (AGRICULTURAL PRODUCT);

CELL EXTRACT; MESSENGER RNA; TUMOR NECROSIS FACTOR RECEPTOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN SYNTHESIS; WHEAT;

ANIMALS; CELL EXTRACTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; RECEPTORS, TUMOR NECROSIS FACTOR; RNA, MESSENGER; TRITICUM;

TRITICUM AESTIVUM;

EID: 67649230845     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-9972-x     Document Type: Article

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