Reversal of coenzyme specificity and improvement of catalytic efficiency of Pichia stipitis xylose reductase by rational site-directed mutagenesis

Biotechnology Letters

Volumn 31, Issue 7, 2009, Pages 1025-1029

  Zeng, Qi Kai ^a   Du, Hong Li ^a   Wang, Jing Fang ^c   Wei, Dong Qing ^b,c   Wang, Xiao Ning ^a   Li, Yi Xue ^c   Lin, Ying ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b SHANGHAI JIAO TONG UNIVERSITY   (China)

^c INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

Catalytic efficiency; Coenzyme specificity; Site directed mutagenesis; Substitution simulation; Xylose reductase

Indexed keywords

CATALYTIC EFFICIENCY; COENZYME SPECIFICITY; SITE-DIRECTED MUTAGENESIS; SUBSTITUTION SIMULATION; XYLOSE REDUCTASE;

ENZYMES; ETHANOL; MUTAGENESIS; SUGAR SUBSTITUTES;

ENZYME ACTIVITY;

ALDEHYDE REDUCTASE; FUNGAL PROTEIN; MOLECULAR MOTOR; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SUBSTITUTION; ARTICLE; COENZYME; ENZYMOLOGY; GENETICS; METABOLISM; PICHIA; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

ALDEHYDE REDUCTASE; AMINO ACID SUBSTITUTION; COENZYMES; FUNGAL PROTEINS; MOLECULAR MOTOR PROTEINS; MUTAGENESIS, SITE-DIRECTED; NAD; NADP; PICHIA; PROTEIN STRUCTURE, TERTIARY;

PICHIA STIPITIS;

EID: 67649221663     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-9980-x     Document Type: Article

References (15)

1. BCH Chu H Lee 2006 Investigation of the role of a conserved glycine motif in the Saccharomyces cerevisiae xylose reductase Curr Microbiol 53 118 123 (Pubitemid 44165839)
2. B Hahn-Hagerdal K Karhumaa C Fonseca 2007 Towards industrial pentose-fermenting yeast strains Appl Microbiol Biotechnol 74 937 953 (Pubitemid 46434767)
3. EY Jeong C Sopher IS Kim 2001 Mutational study of the role of tyrosine-49 in the Saccharomyces cerevisiae xylose reductase Yeast 18 1081 1089 (Pubitemid 32780857)
4. M Jeppsson K Traff B Johansson 2003 Effect of enhanced xylose reductase activity on xylose consumption and product distribution in xylose-fermenting recombinant Saccharomyces cerevisiae FEMS Yeast Res 3 167 175 (Pubitemid 36321735)
5. M Jeppsson O Bengtsson K Franke 2006 The expression of a Pichia stipitis xylose reductase mutant with higher Km for NADPH increases ethanol production from xylose in recombinant Saccharomyces cerevisiae Biotechnol Bioeng 93 665 673
6. S Katahira A Mizuike H Fukuda 2006 Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide- assimilating yeast strain Appl Microbiol Biotechnol 72 1136 1143 (Pubitemid 44564446)
7. M Klimacek M Szekely R Griessler 2001 Exploring the active site of yeast xylose reductase by site-directed mutagenesis of sequence motifs characteristic of two dehydrogenase/reductase family types FEBS Lett 500 149 152 (Pubitemid 32611244)
8. Y Lin S Tanaka 2006 Ethanol fermentation from biomass resources: current state and prospects Appl Microbiol Biotechnol 69 627 642 (Pubitemid 43136522)
9. JN Nigam 2001 Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis J Biotech 87 17 27 (Pubitemid 32232222)
10. B Petschacher S Leitgeb KL Kavanagh 2005 The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography Biochem J 385 75 83 (Pubitemid 40164025)
11. JF Wang DQ Wei Y Lin 2007 Insights from modeling the 3D structure of NAD(P)H-dependent D-xylose reductase of Pichia stipitis and its binding interactions with NAD and NADP Biochem Biophys Res Commun 359 323 329 (Pubitemid 46887686)
12. S Watanabe T Kodaki K Makino 2005 Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zinc J Biol Chem 280 10340 10349 (Pubitemid 40403056)
13. S Watanabe A Abu Saleh SP Pack 2007 Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP(+)-dependent xylitol dehydrogenase J Biotech 130 316 319 (Pubitemid 46920539)
14. S Watanabe SP Pack A Abu Saleh 2007 The positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae Biosci Biotechnol Biochem 71 1365 1369 (Pubitemid 47164260)
15. QK Zeng HL Du ZC Zhai 2008 Mutational research on the role of lysine 21 in the Pichia stipitis xylose reductase Chin J Biotech 24 1108 1111