Single-step purification of native miraculin using immobilized metal-affinity chromatography

Journal of Agricultural and Food Chemistry

Volumn 57, Issue 12, 2009, Pages 5148-5151

  Duhita, Narendra ^a   Hiwasa Tanase, Kyoko ^a   Yoshida, Shigeki ^a   Ezura, Hiroshi ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Immobilized metal affinity chromatography; Miraculin; Purification; Taste modifying protein

Indexed keywords

GLYCOPROTEIN; METAL; MIRACULIN PROTEIN, SYNSEPALUM DULCIFICUM; SWEETENING AGENT; VEGETABLE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; EVALUATION; ISOLATION AND PURIFICATION; METHODOLOGY; MIRACLE FRUIT; PROTEIN BINDING;

CHROMATOGRAPHY, AFFINITY; GLYCOPROTEINS; METALS; PLANT PROTEINS; PROTEIN BINDING; SWEETENING AGENTS; SYNSEPALUM;

SYNSEPALUM DULCIFICUM;

EID: 67649221349     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf9004065     Document Type: Article

References (25)

1. Faus, I. Recent developments in the characterization and biotechnological production of sweet-tasting proteins. Appl. Microbiol. Biotechnol. 2000, 53, 145-151.
2. Masuda, T.; Kitabatake, N. Developments in biotechnological production of sweet proteins. J. Biosci. Bioeng. 2006,102, 375-389.
3. Kurihara, K.; Beidler, L. M. Taste-modifying protein from miracle fruit. Science 1968, 161, 1241-1243.
4. Sun, H. J.; Cui, M. L.; Ma, B.; Ezura, H. Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce. FEBS Lett. 2006, 505, 620-626.
5. Sun, H. J.; Kataoka, H.; Yano, M.; Ezura, H. Genetically stable expression of functional miraculin, a new type of alternative sweetener, in transgenic tomato plants. Plant Biotechnol. J. 2007, 5, 768-777.
6. Sugaya, T.; Yano, M.; Sun, H. J.; Hirai, T.; Ezura, H. Transgenic strawberry expressing the taste-modifying protein miraculin. Plant Biotechnol. 2008, 25, 329-333.
7. Ito, K.; Asakura, T.; Morita, Y.; Nakajima, K.; Koizumi, A.; Shimizu-Ibuka, A.; Masuda, K.; Ishiguro, M.; Terada, T.; Maruyama, J.; Kitamoto, K.; Misaka, T.; Abe, K. Microbial production of sensory-active miraculin. Biochem. Biophys. Res. Commun. 2007, 360, 407-411.
8. Kurihara, Y.; Beidler, L. M. Mechanism of the action of tastemodifying protein. Nature 1969, 222, 1176-1179.
9. Takahashi, N.; Hitotsuya, H.; Hanzawa, H.; Arata, Y.; Kurihara, Y. Structural study of asparagine-linked oligosaccharide moiety of taste-modifying protein, miraculin. J. Biol. Chem. 1989, 265, 7793-7798.
10. Matsuyama, T.; Satoh, M.; Nakata, R.; Aoyama, T.; Inoue, H. Functional expression of miraculin, a taste-modifying protein in Escherichia coli. J. Biochem. 2009, 145, 445-450.
11. Giroux, E. L.; Henkin, R. I. Purification and some properties of miraculin, a glycoprotein from Synsepalum dulciflcum which provokes sweetness and blocks sourness. J. Agrie. Food Chem. 1974, 22, 594-601.
12. Kurihara, Y.; Terasaki, S. Isolation and chemical properties of multiple active principles from miracle fruit. Biochem. Biophys. Acta 1982, 719, 444-449.
13. Brouwer, J. N.; Van der WeI, H.; Francke, A.; Henning, G. J. The sweetness-inducing effect of miraculin; behavioural and neurophysiological experiments in the rhesus monkey Macaca mulatto. Nature 1968, 220, 373-374.
14. Theerasilp, S.; Kurihara, Y. Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit. J. Biol. Chem. 1988, 263, 11536-11539.
15. Paladino, A.; Costantini, S.; Colonna, G.; Facchiano, A. M. Molecular modeling of miraculin: Structural analyses and functional hypotheses. Biochem. Biophys. Res. Commun. 2008, 367, 26-32.
16. Li, N.; Qu, L. J.; Liu, Y.; Li, Q.; Gu, H.; Chen, Z. The refolding, purification, activity analysis of a rice Bowman-Birk inhibitor expressed in Escherichia coli. Protein Expression Purif 1999, 15, 99-104.
17. Lin, G. Z.; Lian, Y. J.; Ryu, J. H.; Sung, M. K.; Park, J. S.; Park, H. J.; Park, B. K.; Shin, J. S.; Lee, M. S.; Cheon, C. I. Expression and purification of His-tagged flavonol synthase of Camellia sinensis from Escherichia coli. Protein Expression Purif 2007, 55, 287-292.
18. Kurihara, Y. Characteristics of antisweet substances, sweet proteins, and sweetness-inducing proteins. Crit. Rev. Food Sei. Nutr. 1992, 32, 231-252.
19. Das, A.; Mukhopadhyay, C. Atomistic mechanism of protein denaturation by urea. J. Phys. Chem. B 2008, 112, 7903-7908.
20. Nakajima, K.; Morita, Y.; Koizumi, A.; Asakura, T.; Terada, T.; Ito, K.; Shimizu-Ibuka, A.; Maruyama, J.; Kitamoto, K.; Misaka, T.; Abe, K. Acid-induced sweetness of neoculin is ascribed to its pHdependent agonistic-antagonistic interaction with human sweet taste receptor. FASEB J. 2008, 22, 2323-2330.
21. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 1976, 72, 248-254.
22. Theerasilp, S.; Hitotsuya, H.; Nakajo, S.; Nakaya, K.; Nakamura, Y.; Kurihara, Y. Complete amino acid sequence and structure characterization of the taste-modifying protein, miraculin. J. Biol. Chem. 1989, 264, 6655-6659.
23. Masuda, Y.; Nirasawa, S.; Nakaya, K.; Kurihara, Y. Cloning and sequencing of a cDNA encoding a taste-modifying protein, miraculin. Gene 1995, 161, 175-177.
24. Kurihara, Y.; Nirasawa, S. Structures and activities of sweetnessinducing substances (miraculin, curculin, strogin) and the heat-stable sweet protein, mabinlin. Foods Food Ingredients J. Jpn. 1997, 174, 61-14.
25. Al-Mashikhi, S. A.; Nakai, S. Separation of ovotransferrin from egg white by immobilized metal affinity chromatography. Agrie. Biol. Chem. 1987, 51, 2881-2887.