Entamoeba histolytica: Cysteine proteinase activity and virulence. Focus on cysteine proteinase 5 expression levels

Experimental Parasitology

Volumn 122, Issue 4, 2009, Pages 306-309

  Freitas, Michelle A R ^a   Fernandes, Helen C ^a   Calixto, Viviane C ^a   Martins, Almir S ^a   Silva, Edward F ^a   Pesquero, Jorge L ^a   Gomes, Maria A ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

Cysteine proteinase 5; Entamoeba histolytica; Pathogenesis of amoebiasis

Indexed keywords

CYSTEINE PROTEINASE; CYSTEINE PROTEINASE 5; MESSENGER RNA; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACTERIUM ISOLATE; CLINICAL FEATURE; CONTROLLED STUDY; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVITY; HAMSTER; LIVER AMEBIASIS; NONHUMAN; PARASITE VIRULENCE; PATHOGENESIS; PRIORITY JOURNAL; TROPHOZOITE;

ANIMALS; CRICETINAE; CYSTEINE ENDOPEPTIDASES; ENTAMOEBA HISTOLYTICA; ENTAMOEBIASIS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; LIVER; LIVER ABSCESS, AMEBIC; MESOCRICETUS; POLYMERASE CHAIN REACTION; RNA, MESSENGER; VIRULENCE;

CRICETINAE; ENTAMOEBA HISTOLYTICA;

EID: 67449161556     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2009.04.005     Document Type: Article

References (25)

1. Ankri S., Stolarsky T., and Mirelman D. Antisense inhibition of expression of cysteine proteinases does not affect Entamoeba histolytica cytopathic or haemolytic activity but inhibits phagocytosis. Molecular Microbiology 28 (1998) 777-785
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72 (1976) 248-254
3. Bruchhaus I., Jacobs T., Leippe M., and Tannich E. Entamoeba histolytica and Entamoeba dispar: differences in numbers and expression of cysteine proteinase genes. Molecular Microbiology 22 (1996) 255-263
4. Bruchhaus I., Loftus B.J., Hall N., and Tannich E. The intestinal protozoan parasite Entamoeba histolytica contains 20 cysteine protease genes, of which only a small subset is expressed during in vitro cultivation. Eukaryotic Cell 2 (2003) 501-509
5. Carvalho R., Passos L.M.F., and Martins A.S. Development of a differential multiplex PCR assay for equine herpes virus 1 and 4 as a diagnostic tool. Journal of Veterinary Medicine 47 (2000) 351-359
6. Diamond L.S., Harlow D.R., and Cunnick C.C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Transaction of the Royal Society of Tropical Medicine and Hygiene 72 (1978) 431-432
7. Diamond L.S., Phillips B.P., and Bartgis I.L. A comparison of the virulence of nine strains of axenically cultivated E. histolytica in hamster liver. Archivos de investigation Medica (Mex) 5 2 (1974) 423-426
8. Freitas M.A., Vianna E.N., Martins A.S., Silva E.F., Pesquero J.L., and Gomes M.A. A single step duplex PCR to distinguish Entamoeba histolytica from Entamoeba dispar. Parasitology 128 (2004) 625-628
9. Garcia-Rivera G., Rodriguez M.A., Ocadiz R., Martinez-Lopez M.C., Arroyo R., Gonzalez-Robles A., and Orozco E. Entamoeba histolytica: a novel cysteine protease and an adhesin form the 112 kDa surface protein. Molecular Microbiology 33 (1999) 556-568
10. Gomes M.A., Pesquero J.B., Furst C., Valle P.R., Pesquero J.L., and Silva E.F. An improved method to distinguish Entamoeba histolytica and Entamoeba dispar. Parasitology 119 Pt 4 (1999) 359-362
11. Keene W.E., Hidalgo M.E., Orozco E., and McKerrow J.H. Entamoeba histolytica: correlation of the cytopathic effect of virulent trophozoites with secretion of a cysteine proteinase. Experimental Parasitology 71 (1990) 199-206
12. Lauwaet T., Oliveira M.J., Callewaert B., De Bruyne G., Saelens X., Ankri S., Vandenabeele P., Mirelman D., Mareel M., and Leroy A. Proteolysis of enteric cell villin by Entamoeba histolytica cysteine proteinases. Journal of Biological Chemistry 278 (2003) 22650-22656
13. Li E., Yang W.G., Zhang T., and Stanley Jr. S.L. Interaction of laminin with Entamoeba histolytica cysteine proteinases and its effect on amebic pathogenesis. Infection and Immunity 63 (1995) 4150-4153
14. Loftus B., Anderson I., Davies R., Alsmark U.C., Samuelson J., Amedeo P., et al. The genome of the protist parasite Entamoeba histolytica. Nature 433 (2005) 865-868
15. Moncada D., Keller K., Ankri S., Mirelman D., and Chadee K. Antisense inhibition of Entamoeba histolytica cysteine proteases inhibits colonic mucus degradation. Gastroenterology 130 (2006) 721-730
16. Moncada D., Keller K., and Chadee K. Entamoeba histolytica cysteine proteinases disrupt the polymeric structure of colonic mucin and alter its protective function. Infection and Immunity 71 (2003) 838-844
17. Nowak N., Lotter H., Tannich E., and Bruchhaus I. Resistance of Entamoeba histolytica to the cysteine proteinase inhibitor E64 is associated with secretion of pro-enzymes and reduced pathogenicity. Journal of Biological Chemistry 279 (2004) 38260-38266
18. Olivos-Garcia A., Tello E., Nequiz-Avendano M., Gonzalez-Canto A., Lopez-Vancell R., Garcia de Leon M.C., Montfort I., and Perez-Tamayo R. Cysteine proteinase activity is required for survival of the parasite in experimental acute amoebic liver abscesses in hamsters. Parasitology 129 (2004) 19-25
19. Que X., and Reed S.L. The role of extracellular cysteine proteinases in pathogenesis of Entamoeba histolytica invasion. Parasitology Today 13 (1997) 190-194
20. Reed S.L., Ember J.A., Herdman D.S., DiScipio R.G., Hugli T.E., and Gigli I. The extracellular neutral cysteine proteinase of Entamoeba histolytica degrades anaphylatoxins C3a and C5a. The Journal of Immunology 155 (1995) 266-274
21. Reed S.L., Keene W.E., and McKerrow J.H. Thiol proteinase expression and pathogenicity of Entamoeba histolytica. Journal of Clinical Microbiology 27 (1989) 2772-2777
22. Silva E.F., and Mayrink W. Estudos sobre a Entamoeba moshkovskii. Novos focos em diversos tipos de coleções hídricas no Brasil e Uruguai. Revista do Instituto de Medicina Tropical de São Paulo 16 (1974) 203-221
23. Stanley Jr. S.L., Zhang T., Rubin D., and Li E. Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice. Infection and Immunity 63 (1995) 1587-1590
24. Willhoeft U., Hamann L., and Tannich E. A DNA sequence corresponding to the gene encoding cysteine proteinase 5 in Entamoeba histolytica is present and positionally conserved but highly degenerated in Entamoeba dispar. Infection and Immunity 67 (1999) 5925-5929
25. Zhang Z., Wang L., Seydel K.B., Li E., Ankri S., Mirelman D., and Stanley Jr. S.L. Entamoeba histolytica cysteine proteinases with interleukin-1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis. Molecular Microbiology 37 (2000) 542-548