An improved method to distinguish Entamoeba histolytica and Entamoeba dispar

Parasitology

Volumn 119, Issue 4, 1999, Pages 359-362

  Gomes, M A ^a   Pesquero, J B ^b   Furst, C ^a   Valle, P R ^a   Pesquero, J L ^a   Silva, E F ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Diagnosis; DNA conformation; DNA electrophoresis; Entamoeba histolytica Entamoeba dispar differentiation; PCR

Indexed keywords

DNA FRAGMENT; POLYACRYLAMIDE GEL;

ARTICLE; DNA CONFORMATION; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; GEL ELECTROPHORESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTOZOAL INFECTION;

ANIMALS; ENTAMOEBA; ENTAMOEBA HISTOLYTICA; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ANALYSIS, DNA;

ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; PROTOZOA;

EID: 0032833136     PISSN: 00311820     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0031182099004886     Document Type: Article

References (18)

1. BOLSHOY, A., McNAMARA, P., HARRINGTON, R. E. & TRIFONOV, E. N. (1991). Curved DNA without A-A: experimental estimation of all 16 DNA wedge angles. Proceedings of the National Academy of Sciences, USA 88, 2312-2316.
2. CLARK, C. G. & DIAMOND, L. S. (1991). Ribosomal RNA genes of pathogenic and nonpathogenic Entamoeba histolytica are distinct. Molecular and Biochemical Parasitology 104, 239-246.
3. DIAMOND, L. S., HARLOW, D. R. & CUNNICK, C. C. (1978). A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 170-176.
4. ENGLUND, P. T. & MARINI, J. C. (1980). The replication of kinetoplast DNA. American Journal of Tropical Medicine and Hygiene 29, 1064-1069.
5. FARRI, T., SARGEAUNT, P., WARHURST, D. & WILLIAMS, J. (1979). Electrophoretic isoenzyme patterns of the pathogenic and nonpathogenic intestinal amoebae of man. Transactions of the Royal Society of Tropical Medicine and Hygiene 73, 225-227.
6. GABRIELIAN, A., VLAHOVICEK, K. & PONGOR, S. (1997). Distribution of sequence-dependent curvature in genomic DNA sequences. FEBS Letters 406, 69-74.
7. GOMES, M. A., SILVA, E. F., MACEDO, A. M., VAGO, A. R. & MELO, M. N. (1997). LSSP-PCR for characterization of strains of Entamoeba histolytica isolated in Brazil. Parasitology 114, 517-520.
8. HAQUE, R., NEVILLE, L. M., HAHN, P. & PETRI, W. A. JR. (1995). Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits. Journal of Clinical Microbiology 33, 2558-2561.
9. MARTINEZ-PALOMO, A. (1987). The pathogenesis of amoebiasis. Parasitology Today 3, 111-118.
10. MIRELMAN, D., NUCHAMOWITZ, Y. & STOLARSKY, T. (1997). Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection of Entamoeba histolytica and E. dispar. Journal of Clinical Microbiology 35, 2405-2407.
11. MOODY, S., BECKER, S., NUCHAMOWITZ, Y. & MIRELMAN, D. (1997). Virulent and avirulent Entamoeba histolytica and E. dispar differ in their cell surface phosphorylated glycolipids. Parasitology 114, 95-104.
12. NOVATI, S., SIRONI, M., GRANATA, S., BRUNO, A., GATTI, S. & SCAGLIA, M. (1996). Direct sequencing of the PCR amplified SSU rRNA genes of Entamoeba dispar and the design of primers for rapid differentiation from Entamoeba histolytica. Parasitology 112, 363-369.
13. SAAD, F. A., HALLIGER, B., MULLER, C. R., ROBERTS, R. G. & DANIELI, G. A. (1994). Single base substitutions are detected by double strand conformation analysis. Nucleic Acids Research 22, 4352-4353.
14. SAMBROOK, J., FRITSCH, E. F. & MANIATIS, T. (1989). Molecular Cloning. A Laboratory Manual, 2nd Edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. SANTOS, F. R., PENA, S. D. J. & EPLLEN, J. T. (1993). Genetic and populational study of a Y-linked tetranucleotide repeat DNA polymorphism with a simple non-isotopic technique. Human Genetics 90, 655-656.
16. SARGEAUNT, P. G., WILLIAMS, J. E. & GRENE, J. D. (1978). The differentiation of invasive and non-invasive Entamoeba histolytica by isoenzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 519-521.
17. TACHIBANA, H., KOBAYASHI, S., PAZ, K. C., ACA, I. S., TATENTO, S. & ILHARA, S. (1992). Analysis of pathogenicity by restriction-endonuclease digestion of amplified genomic DNA of Entamoeba histolytica isolated in Pernambuco, Brazil. Parasitology Research 78, 433-436.
18. TANNICH, E. & BURCHARD, G. D. (1991). Differentiation of pathogenic from nonpathogenic Entamoeba histolytica by restriction fragment analysis of a single gene amplified'In vitro'. Journal of Clinical Microbiology 29, 250-255.