A novel method using immuno-affinity chromatography for isolating β-conglycinin from soybean proteins

Food Chemistry

Volumn 117, Issue 2, 2009, Pages 371-374

  You, Jinming ^a   Sun, Peng ^b   Li, Defa ^b   Qiao, Shiyan ^b   Qu, Mingren ^a   Wang, Zirui ^a   Li, Guanhong ^a   Pan, Ke ^a  

^a JIANGXI AGRICULTURAL UNIVERSITY   (China)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Conglycinin; Immuno affinity chromatography; Monoclonal antibody (Mab); Purification

Indexed keywords

BETA CONGLYCININ; EPITOPE; GLYCININ; MONOCLONAL ANTIBODY; OVALBUMIN; SOYBEAN PROTEIN; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; BIOLOGICAL ACTIVITY; CHICKEN; CONJUGATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FOOD ALLERGY; IMMUNOAFFINITY CHROMATOGRAPHY; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN PURIFICATION; SOYBEAN; WESTERN BLOTTING;

GLYCINE MAX;

EID: 67349284702     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodchem.2009.03.098     Document Type: Article

References (20)

1. Astadi, I. R., Astuti, M., Santoso, U., & Nugraheni, P. S. (2008). In vitro antioxidant activity of anthocyanins of black soybean seed coat in human low density lipoprotein (LDL). Food Chemistry, doi: 10.1016/j.foodchem.2008.06.034.
2. Chandra R.K. Food hypersensitivity and allergic diseases. European Journal of Clinical Nutrition 56 Suppl. 3 (2002) 54-56
3. Friedman M., and Brandon D.L. Nutritional and health benefits of soy proteins. Journal of Agricultural and Food Chemistry 49 (2001) 1069-1086
4. Guo P., Piao P., Cao Y., Ou D., and Li D. Recombinant soybean protein β-conglycinin α′-subunit expression and induced hypersensitivity reaction in rats. International Archieves of Allergy and Immunolgy 145 (2008) 102-110
5. Herman E.M., Helm R.M., Jung R., and Kinney A.J. Genetic modification removes an immunodominant allergen from soybean. Plant Physiology 132 (2003) 36-43
6. Huisman J., and Jansman A.J.M. Dietary effects and some analytical aspects of antinutritional factors in peas (Pisum sativum), common beans (Phaseolus vulgaris) and soyabeans (Glycine max L) in monogastric farm animals. A literature review. Nutrition Abstract and Reviews (Series B) 61 (1991) 901-921
7. 4. Nature 207 (1970) 680-685
8. Lallès J.P., and Dreau D. Identification of soyabean allergens and immune mechanisms of dietary sensitivities in prerumminant calves. Research in Veterinary Science 60 (1996) 111-116
9. Li D.F., Nelssen J.L., Reddy P.G., Blecha F., Hancock J.D., Allee G.L., et al. Transient hypersensitivity to soybean meal in the early weaned pig. Journal of Animal Science 68 (1990) 1790-1799
10. Liu C., Wang X., Ma H., Zhang Z., Gao W., and Xiao L. Functional properties of protein isolates from soybeans stored under various conditions. Food Chemistry 111 1 (2008) 29-37
11. Lovati M.R., Manzoni C., Corsini A., Granata A., Frattini R., Fumagalli R., et al. Low density lipoprotein receptor activity is modulated by soybean globulins in cell culture. Journal of Nutrition 122 (1992) 1971-1978
12. Nelson P.N., Reynolds G.M., Waldron E.E., Ward E., Giannopoulos K., and Murry P.G. Monoclonal antibodies demystified. Journal of Clinical Pathology 53 (2000) 111-118
13. Rickert D.A., Johnson L.A., and Murphy P.A. Improved fractionation of glycinin and β-conglycinin and partitioning of phytochemicals. Journal of Agriculture and Food Chemistry 52 (2004) 1726-1734
14. Saito T., Kohno M., Tsumura K., Kugimiya W., and Kito M. Novel method using phytase for separating soybean β-conglycinin and glycinin. Bioscience Biotechnology and Biochemistry 65 (2001) 884-887
15. Song Y.S., Frias J., Martinez-Villaluenga C., Vidal-Valdeverde C., and de Mejia E.G. Immunoreactivity reduction of soybean meal by fermentation, effect on amino acid composition and antigenicity of commercial soy products. Food Chemistry 108 2 (2008) 571-581
16. Thanh V.H., and Shibasaki K. Major proteins of soybean seed: A straightforward fractionation and their characterisation. Journal of Agricultural and Food Chemistry 24 (1976) 1117-1121
17. Wu S.W., Murphy P.A., Reuber M.A., and Fratzke A.R. Simplified process for soybean glycinin and β-conglycinin fractionation. Journal of Agriculture and Food Chemistry 48 (2000) 2702-2708
18. Xiang P., Beardslee T.A., Zeece M.G., Marwell J., and Sarath G. Identification and analysis of a conserved immunoglobulin E-binding epitope in soybean G1a and G2a and peanut Ara h 3 glycinins. Archives of Biochemistry and Biophysics 408 (2002) 51-57
19. You J.M., Li D.F., Qiao S.Y., Wang Z.R., He P.L., Ou D.Y., et al. Development of monoclonal antibody-based competitive ELISA for detection of β-conglycinin, an allergen from soybean. Food Chemistry 106 1 (2008) 352-360
20. Zeiger R.S. Dietary aspects of food allergy prevention in infants and children. Journal of Pediatric Gastroenterology and Nutrition 30 (2000) 77-86