DNA shuffling of methionine adenosyltransferase gene leads to improved S-adenosyl-l-methionine production in Pichia pastoris

Journal of Biotechnology

Volumn 141, Issue 3-4, 2009, Pages 97-103

  Hu, Hui ^a   Qian, Jiangchao ^a   Chu, Ju ^a   Wang, Yong ^a   Zhuang, Yingping ^a   Zhang, Siliang ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

DNA shuffling; Methionine adenosyltransferase; Pichia pastoris; S adenosyl l methionine

Indexed keywords

CELL FUNCTIONS; CEREVISIAE; DNA SHUFFLING; INDUSTRIAL-SCALE PRODUCTIONS; METHIONINE ADENOSYLTRANSFERASE; P. PASTORIS; PICHIA PASTORIS; RECOMBINANT STRAINS; S-ADENOSYL-L-METHIONINE;

DNA; ESCHERICHIA COLI; GENES; NUCLEIC ACIDS; STRAIN;

ENZYME ACTIVITY;

DNA; ENZYME VARIANT; METHIONINE ADENOSYLTRANSFERASE; S ADENOSYLMETHIONINE;

ARTICLE; BACTERIAL GENE; BIOREACTOR; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA RECOMBINATION; DNA SHUFFLING; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; FUNGAL STRAIN; FUNGUS CULTURE; GENE EXPRESSION; GENE MUTATION; GENE SEQUENCE; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; RECOMBINANT GENE; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; STREPTOMYCES; STREPTOMYCES SPECTABILIS; STRUCTURAL HOMOLOGY; STRUCTURE ACTIVITY RELATION;

BIOREACTORS; DNA SHUFFLING; ESCHERICHIA COLI; METHIONINE ADENOSYLTRANSFERASE; MODELS, MOLECULAR; MUTATION; PICHIA; RECOMBINANT PROTEINS; S-ADENOSYLMETHIONINE; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN; STREPTOMYCES;

ESCHERICHIA COLI; PICHIA PASTORIS; STREPTOMYCES SPECTABILIS;

EID: 67349271073     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.03.006     Document Type: Article

References (32)

1. Bruno B., Park J.B., Lars M.B., and Schmid A. NADH availability limits asymmetric biocatalytic epoxidation in a growing recombinant Escherichia coli strain. Appl. Environ. Microbiol. 74 5 (2008) 1436-1446
2. Cereghino J.L., and Cregg J.M. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24 (2000) 45-66
3. Chan S.Y., and Appling D.R. Regulation of S-adenosylmethionine levels in Saccharomyces cerevisiae. J. Biol. Chem. 278 (2003) 43051-43059
4. Chen H.X., Chu J., Zhang S.L., Zhuang Y.P., Qian J.C., Wang Y.H., and Hu X.Q. Intracellular expression of Vitreoscilla hemoglobin improves S-adenosylmethionine production in a recombinant Pichia pastoris. Appl. Microbiol. Biotechnol. 74 6 (2007) 1205-1212
5. Crameri A., Raillard S.A., Bermudez E., and Stemmer W.P. DNA shufling of a family of genes from diverse species accelerates directed evolution. Nature 391 6664 (1998) 288-291
6. Emond S., Andre I., Jaziri K., Potocki-Veronese G., Mondon P., Bouayadi K., Kharrat H., Monsan P., and Remaud-Simeon M. Combinatorial engineering to enhance thermostability of amylosucrase. Protein Sci. 17 (2008) 967-976
7. Favre-Bulle O., and Witholt B. Biooxidation of n-octane by a recombinant Escherichia coli in a two-liquid-phase system: effect of medium components on cell growth and alkane oxidation activity. Enzyme Microb. Technol. 14 (1992) 931-937
8. Hu X.Q., Chu J., Zhang S.L., Zhuang Y.P., Wang Y.H., Zhu S., Zhu Z.G., and Yuan Z.Y. A novel feeding strategy during the production phase for enhancing the enzymatic synthesis of S-adenosyl-l-methionine by methylotrophic Pichia pastoris. Enzyme Microb. Technol. 40 (2007) 669-674
9. John C.T., and George D.M. The bifunctional active site of S-adenosylmethionine synthetase. J. Biol. Chem. 274 46 (1999) 32909-32914
10. Li D.Y., Yu J., Tian L.W., Ji X.S., and Yuan Z.Y. Production of SAM by recombinant Pichia pastoris. Chin. J. Biotechnol. 3 (2002) 295-299
11. Liu P.Y., Zhu D.H., and Tan T.W. Effect of feeding pre-l-methionine on high-cell-density fermentation for S-adenosyl-l-methionine production. Chin. J. Biotechnol. 22 2 (2006) 268-272
12. Liu Z.Q., Sun Z.H., Zheng P., Leng Y.J., and Qian S. Directed evolution of d-lactonohydrolase by error prone PCR and DNA shuffling. Sheng Wu Gong Cheng Xue Bao 21 5 (2005) 773-781
13. Lu S.C. S-adenosylmethionine. Int. J. Biochem. Cell Biol. 32 (2000) 391-395
14. Mato, J.M., Pajares, M.A., Mingorance, J., Avarez, L., 1995. Production of S-adenosyl-methionine (SAM) by fermentation of transformed bacteria. European patent 0647712A1.
15. Mautino M.R., Barra J.L., and Rosa A.L. eth-1, the neurospora crassa locus encoding S-adenosylmethionine synthetase: molecular cloning, sequence analysis and in vivo overexpression. Genetics 142 3 (1996) 789-800
16. Megumi S., Tsutomu F., and Haruyuki I. Effects of accumulated S-adenosylmethionine on growth of yeast cells. Biosci. Biotechnol. Biochem. 71 6 (2007) 1595-1597
17. Mincheva K.P., and Balutsov V.M. Isolation of S-adenosyl-l-methionine from an enriched Kluyveromyces lactis mutant grown in whey media. Prikl. Biokhim. Mikrobiol. 38 4 (2002) 389-392
18. Park J.H., Tai J.Z., Roessner C.A., and Sott A.T. Enzymatic synthesis of S-adenosyl-l-methionine on the preparative scale. Bioorg. Med. Chem. 4 12 (1996) 2179-2185
19. Ryu K., Kang J.H., Wang L., and LeeJ E.K. Expression in yeast of secreted lignin peroxidase with improved 2 4-dichlorophenol degradability by DNA shuffling. Biotechnology 135 3 (2008) 241-246
20. Sambrook J., and Russell D.W. Molecular Cloning:A Laboratory Manual. third ed. (2001), Cold Spring Harbor Laboratory Press, New York, NY
21. Scorer C.A., Clare J.J., McCombie W.R., Michael A., Romanos, and Sreekrishna K. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. Biotechnology 12 2 (1994) 181-184
22. Shiozaki S., Shimizu S., and Yamaha H. Unusual intracellular accumulation of S-adenosyl-l-methionine by microorganisms. Arric. Biol. Chem. 48 (1984) 2293-2300
23. Shiozaki S., Shimizu S., and Yamada H. Production of S-adenosyl-l-methionine by Saccharomces sake. J. Biotechnol. 4 6 (1986) 345-354
24. Shobayashi M., Mukai N., Iwashita K., Hiraga Y., and lefuji H. A new method for isolation of S-adenosylmethionine (SAM)-accumulating yeast. Appl. Microbiol. 69 (2005) 704-710
25. Stemmer W.P.C. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. 91 (1994) 10747-10751
26. Suen W.C., Zhang N., Xiao L., Madison V., and Zaks A. Improved activity and thermo-stability of Candida antarctica lipase B by DNA family shuffling. Protein. Eng. Des. Sel. 7 2 (2004) 133-140
27. Takusagawa F., Kamitori S., Misaki S., and George D.M. Crystal structure of S-adenosylmethionine synthetase. J. Biol. Chem. 271 1 (1996) 136-147
28. Tatsuya T., and Yoshinori H. Development of innovative Pediocin PA-1 by DNA Shuffl{ligature}ing among Class IIa Bacteriocins. Appl. Environ. Microbiol. 73 (2007) 5292-5299
29. Wanger J., Danzin C., Huot-Olivier S., Claveric N., and Palfreyman M.G. High performance liquid chromatographic analysis of S-adenisylmethionine and its metabolites in rat tissues: interrelationship with changes in biogenic catechol levels following treatment with l-dopa. J. Chromatogr. 290 (1984) 247-262
30. Yang J., Wang M., Sun J., Wei P.H., and Tang W.G. Cloning of S-adenosyl-l-methionine synthetase gene from Saccharomyces ceverisiae and its expression in E. coli. J. China Pharm. Univ. 3 (2002) 253-255
31. Yu Z.L., Wu X.J., Li D.Y., Yang S., Zhou Z., and Cai J.Z.Y. Enhancement of the production of SAM by overexpression of SAM synthetase in Pichia pastoris. Sheng Wu Wu Li Xue Bao, Shanghai 35 (2003) 127-132
32. Zhao H.M., and Frances H.A. Optimization of DNA shuffling for high fidelity recombination. Nucleic Acids Res. 25 6 (1997) 1307-1308