Cloning of serine protease cDNAs from Crotalus durissus terrificus venom gland and expression of a functional Gyroxin homologue in COS-7 cells

Toxicon

Volumn 54, Issue 2, 2009, Pages 110-120

  Yonamine, C M ^a   Prieto da Silva, A R B ^b   Magalhaes G S ^b   Radis Baptista G ^c   Morganti, L ^a   Ambiel, F C ^a   Chura Chambi, R M ^a   Yamane, T ^a   Camillo, M A P ^a  

^a INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES   (Brazil)

^b INSTITUTO BUTANTAN   (Brazil)

^c FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

Author keywords

COS 7 cells; Crotalus durissus; Gyroxin; Protein expression; Recombinant toxin; Serine protease

Indexed keywords

COMPLEMENTARY DNA; PROTEIN GYROXIN; PROTEIN HYGROA; PROTEIN PED; PROTEIN PSECTAG2; SERINE PROTEINASE; SNAKE VENOM; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL STRAIN COS7; CONTROLLED STUDY; CROTALUS DURISSUS TERRIFICUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SEQUENCE HOMOLOGY; SNAKE;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; COS CELLS; CROTALID VENOMS; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESTERASES; EXOCRINE GLANDS; GENE LIBRARY; GENETIC VECTORS; MICE; MOLECULAR WEIGHT; PLASMIDS; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES;

CROTALUS DURISSUS; CROTALUS DURISSUS TERRIFICUS; MAMMALIA; MUS;

EID: 67349267702     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2009.03.022     Document Type: Article

References (50)

1. Alexander G., Grothusen J., Zepeda H., and Schwartzman R.J. Gyroxin, a toxin from the venom of Crotalus durissus terrificus is a thrombin-like enzyme. Toxicon 26 10 (1988) 953-960
2. Arocas V., Castro H.C., Zingali R.B., Guillin M.C., Jandrot-Perrus M., Bon C., and Wisner A. Molecular cloning and expression of bothrojaracin, a potent thrombin inhibitor from snake venom. Eur. J. Biochem. 248 2 (1997) 550-557
3. Barrabin H., Martiarena J.L., Vidal J.C., and Barrio A. Isolation and characterization of Gyroxin from Crotalus durissus terrificus venom. In: Rosemberg P. (Ed). Toxins: Animals, Plant and Microbial (1978), Pergamon Press, New York, USA 113-133
4. Bendtsen J.D., Nielsen H., Von Heijne G., and Brunak S. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340 4 (2004) 783-795
5. Bjarnason J.B., Barish A., Direnzo G.S., Campbell R., and Fox J.W. Kallikrein-like enzymes from Crotalus atrox venom. J. Biol. Chem. 258 20 (1983) 12566-12573
6. Burkhart W., Simth G.F., Su J.L., Parikh I., and LeVine H.I.I.I. Amino acid sequence determination of ancrod, the thrombin-like alpha-fibrinogenase from the venom of Akistrodon rhodostoma. FEBS Lett. 297 3 (1992) 297-301
7. Camillo M.A.P., Alves da Silva J.A., Yonamine C.M., Hashizume J.L., Casare M.S., and Higa O.Z. Biological activity and structural alterations in Gyroxin induced by gamma radiation. International Nuclear Atlantic Conference Proceedings of VII ENAN (2005), Brazilian Association for Nuclear Energy - ABEN, Santos - SP 1845-1850
8. Camillo M.A.P., Arruda Paes P.C., Troncone L.R., and Rogero J.R. Gyroxin fails to modify in vitro release of labelled dopamine and acetylcholine from rat and mouse striatal tissue. Toxicon 39 6 (2001) 843-853
9. Charbonnier F., Gaspera B.D., Armand A.S., Van der Laarse W.J., Launay T., Becker C., Gallien C.L., and Chanoine C. Two myogenin-related genes are differentially expressed in Xenopus laevis myogenesis and differ in their ability to transactivate muscle structural genes. J. Biol. Chem. 277 2 (2002) 1139-1147
10. Chura-Chambi R.M., Tornieri P.H., Spencer P.J., Nascimento P.A., Mathor M.B., and Morganti L. High-level synthesis of recombinant murine endostatin in Chinese hamster ovary cells. Protein. Expr. Purif. 35 1 (2004) 11-16
11. Da Silva N.J., Aird S.D., Seebart C., and Kaiser I.I. A analog from the venom of the bushmaster (Lachesis muta muta). Toxicon 27 7 (1989) 763-771
12. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 4 (1985) 783-791
13. Felsenstein J. PHYLIP - Phylogeny Inference Package (Version 3.2). Cladistics 5 (1989) 164-166 (Felsenstein, J. 2005 PHYLIP - Phylogeny Inference Package Version 3.6. Distributed by the author. Department of Genome Science, University of Washington, Seatle)
14. Hahn B.S., Baek K., Kim W.S., Lee C.S., Chang I.L., and Kim Y.S. Molecular cloning of capillary permeability-increasing enzyme-2 from Agkistrodon caliginosus (Korean viper). Toxicon 36 12 (1998) 1887-1893
15. Hahn B.S., Yang K.Y., Park E.M., Chang I.M., and Kim Y.S. Purification and molecular cloning of calobin, a thrombin-like enzyme from Agkistrodon caliginosus (Korean viper). J. Biochem. 119 5 (1996) 835-843
16. Henschen-Edman A.H., Theodor I., Edwards B.F., and Pirkle H. Crotalase, a fibrinogen-clotting snake venom enzyme: primary structure and evidence for a fibrinogen recognition exosite different from thrombin. Thromb. Haemost. 81 1 (1999) 81-86
17. Hung C.C., and Chiou S.H. Expression of a kallikrein-like protease from the snake venom: engineering of autocatalytic site in the fusion protein to facilitate protein refolding. Biochem. Biophys. Res. Commun. 275 3 (2000) 924-930
18. Itoh N., Tanaka N., Funakoshi I., Kawasaki T., Mihashi S., and Yamashina I. The complete nucleotide sequence of the gene for batroxobin, a thrombin-like snake venom enzyme. Nucleic Acids Res. 16 21 (1988) 10377-10378
19. Itoh N., Tanaka N., Mihashi S., and Yamashina I. Molecular cloning and sequence analysis of cDNA for batroxobin, a thrombin-like snake venom enzyme. J. Biol. Chem. 262 7 (1987) 3132-3135
20. Johansen M.B., Kiemer L., and Brunak S. Analysis and prediction of mammalian protein glycation. Glycobiology 16 9 (2006) 844-853
21. Koh D.C.I., Armugan A., and Jeyaseelan K. Snake venom components and their applications in biomedicine. Cell. Mol. Life. Sci. 63 24 (2006) 3030-3041
22. Kunkel T.A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82 2 (1985) 488-492
23. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 5259 (1970) 680-685
24. Lochnit G., and Geyer R. Carbohydrate structure analysis of batroxobin, a thrombin-like serine protease from Bothrops moojeni venom. Eur. J. Biochem. 228 3 (1995) 805-816
25. Maeda M., Satoh S., Suzuki S., Niwa M., Itoh N., and Yamashina I. Expression of cDNA for batroxobin, a thrombin-like enzyme. J. Biochem. 109 4 (1991) 632-637
26. Magalhaes A., Da Fonseca B.C.B., Diniz C.R., Gilroy J., and Richardson M. The complete amino acid sequence of a thrombin-like enzyme/gyroxin analogue from venom of the bushmaster snake (Lachesis muta muta). FEBS Lett. 329 1-2 (1993) 116-120
27. Mcmullen B.A., Fujikawa K., and Kisiel W. Primary structure of a protein C activator from Agkistrodon contortrix contortrix venom. Biochemistry 28 2 (1989) 674-679
28. Nikai T., Ohara A., Komori Y., Fox J.W., and Sugihara H. Primary structure of a coagulant enzyme, bilineobin, from Agkistrodon bilineatus venom. Arch. Biochem. Biophys. 318 1 (1995) 89-96
29. Nolan C., Hall L.S., and Barlow G.H. Ancrod, the coagulating enzyme of Malayan pitviper (Agkistrodon rhodostoma) venom. Meth. Enzymol. 45 (1976) 205-213
30. Pan H., Du X., Yang G., Zhou Y., and Wu X. cDNA cloning and expression of acutin. Biochem. Biophys. Res. Commun. 255 2 (1999) 412-415
31. Parry M.A., Jacob U., Huber R., Wisner A., Bon C., and Bode W. The crystal structure of the novel snake venom plasminogen activator TSV-PA: a prototype structure for snake venom serine proteinases. Structure 6 9 (1998) 1195-1206
32. Peroni C.N., Soares C.R.J., Gimbo E., Morganti L., Ribela M.T.C.P., and Bartolini P. High-level expression of human thyroid-stimulating hormone in Chinese hamster ovary cells by co-transfection of dicistronic expression vectors followed by a dual-marker amplification strategy. Biotechnol. Appl. Biochem. 35 Pt 1 (2002) 19-26
33. Rádis-Baptista G., Oguiura N., Hayashi M.A., Camargo M.E., Grego K.F., Oliveira E.B., and Yamane T. Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake (Crotalus durissus terrificus). Toxicon 37 7 (1999) 973-984
34. Raw I., Rocha M.C., Esteves M.I., and Kamiguti A.S. Isolation and characterization of a thrombin-like enzyme from the venom of Crotalus durissus terrificus. Braz. J. Med. Biol. Res. 19 3 (1986) 333-338
35. Saitou N., and Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 4 (1987) 406-425
36. Sanchez E.F., Santos C.I., Magalhaes A., Diniz C.R., Figueiredo S., Gilroy J., and Richardson M. Isolation of a proteinase with plasminogen-activating activity from Lachesis muta muta (bushmaster) snake venom. Arch. Biochem. Biophys. 378 1 (2000) 131-141
37. Sanger N., Nicklen J., and Coulson A.R. DNA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. 74 12 (1977) 5463-5467
38. Schuler G.D., Altschul S.F., and Lipman D.J. A workbench for multiple alignment construction and analysis. Proteins 9 3 (1991) 180-190
39. Serrano S., and Maroun R.C. Snake venom serine proteinases: sequence homology vs. substrate specificity, a paradox to be solved. Toxicon 45 8 (2005) 1115-1132
40. Serrano S.M., Hagiwara Y., Murayama N., Higuchi S., Mentele R., Sampaio C.A., Camargo A.C., and Fink E. Purification and characterization of a kinin-releasing and fibrinogen-clotting serine proteinase (KN-BJ) from the venom of Bothrops jararaca and molecular cloning and sequence analysis of its cDNA. Eur. J. Biochem. 251 3 (1998) 845-853
41. Serrano S.M.T., Mentele R., Sampaio C.A.M., and Fink E. Purification, characterization, and amino acid sequence of a serine proteinase, PA-BJ, with platelet-aggregating activity from the venom of Bothrops jararaca. Biochemistry 34 21 (1995) 7186-7193
42. Shimokawa K., and Takahashi H. Capillary permeability-increasing enzyme-2 from the venom of Agkistrodon caliginosus (kankoku-mamushi): activity resulting from the release of peptides from fibrinogen. Toxicon 35 4 (1997) 597-605
43. Soares C.R., Morganti L., Miloux B., Lupker J.H., Ferrara P., and Bartolini P. High-level synthesis of human prolactin in Chinese Hamster ovary cells. Biotechnol. Appl. Biochem. 32 Pt 2 (2000) 127-135
44. Stocker K., Fischer H., Meier J., Brogli M., and Svendsen L. Characterization of the protein C activator Protac from venom of the southern copperhead (Agkistrodon contortrix) snake. Toxicon 25 3 (1987) 239-252
45. Tamura K., Dudley J., Nei M., and Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24 8 (2007) 1596-1599
46. Tokunaga F., Nagasawa K., Tamura S., Miyata T., Iwanaga S., and Kisiel W. The factor V-activating enzyme (RVV-V) from Russell's viper venom. Identification of isoproteins RVV-V alpha, -V beta, and -V gamma and their complete amino acid sequences. J. Biol. Chem. 263 33 (1988) 17471-17481
47. Wang Y.M., Wang S.R., and Tsai I.H. Serine protease isoforms of Deinagkistrodon acutus venom: cloning, sequencing and phylogenetic analysis. Biochem. J. 354 Pt 1 (2001) 161-168
48. You W.K., Choi W.S., Koh Y.S., Shin H.C., Jang Y., and Chung K.H. Functional characterization of recombinant batroxobin, a snake venom thrombin-like enzyme, expressed from Pichia pastoris. FEBS Lett. 571 1-3 (2004) 67-73
49. Zhang Y., Wisner A., Xiong Y., and Bon C. A novel plasminogen activator from snake venom. Purification, characterization, and molecular cloning. J. Biol. Chem. 270 17 (1995) 10246-10255
50. Zhu Z., Liang Z., Zhang T., Zhu Z., Xu W., Teng M., and Niu L. Crystal structures and amidolytic activities of two glycosylated snake venom serine proteinases. J. Biol. Chem. 280 11 (2005) 10524-10529