Biochemical characterization of a metagenome-derived decarboxylase

Enzyme and Microbial Technology

Volumn 45, Issue 1, 2009, Pages 58-63

  Jiang, Chengjian ^a   Shen, Peihong ^a   Yan, Bing ^b   Wu, Bo ^a  

^a GUANGXI UNIVERSITY   (China)

^b Guangxi Institute of Oceanology   (China)

Author keywords

Biocatalyst; Biochemical properties; Decarboxylase; Soil metagenome

Indexed keywords

AMINO ACID RESIDUES; BIOCHEMICAL CHARACTERIZATION; BIOCHEMICAL PROPERTIES; CATALYTIC DOMAINS; DECARBOXYLASE; L-CYSTEINE; METAGENOME; METAGENOMIC LIBRARIES; MUTATION ANALYSIS; SOIL MICRO-ORGANISMS;

AMINATION; AMINES; AMINO ACIDS; BINDING ENERGY; BIOCATALYSTS; CHROMATOGRAPHIC ANALYSIS; CLONING; ENZYMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ORGANIC ACIDS; SOILS;

BINDING SITES;

CARBOXYLYASE; CYSTEINE; CYSTEINE DECARBOXYLASE; MAGNESIUM ION; MERCAPTAMINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DECARBOXYLATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METAGENOMICS; MUTATIONAL ANALYSIS; NUCLEOTIDE SEQUENCE; PH; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SOIL MICROFLORA; SOIL POLLUTION; TEMPERATURE;

EID: 67349249865     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2009.03.003     Document Type: Article

References (20)

1. Kowalchuk G.A., Speksnijder A.G., Zhang K., Goodman R.M., and van Veen J.A. Finding the needles in the metagenome haystack. Microb Ecol 53 (2007) 475-485
2. Torsvik V., and Ovreas L. Microbial diversity and function in soil: from genes to ecosystems. Curr Opin Microbiol 5 (2002) 240-245
3. Van Lanen S.G., and Shen B. Microbial genomics for the improvement of natural product discovery. Curr Opin Microbiol 9 (2006) 252-260
4. Handelsman J. Metagenomics: application of genomics to uncultured microorganisms. Microbiol Mol Biol Rev 68 (2004) 669-685
5. Morris R.M. Environmental genomics: exploring ecological sequence space. Curr Biol 16 (2006) R499-501
6. Dubey S.K., Tripathi A.K., and Upadhyay S.N. Exploration of soil bacterial communities for their potential as bioresource. Bioresour Technol 97 (2006) 2217-2224
7. Rollinger J.M., Langer T., and Stuppner H. Strategies for efficient lead structure discovery from natural products. Curr Med Chem 13 (2006) 1491-1507
8. Shieh C.H., Hong C.J., Huang Y.H., and Tsai S.J. Potential antidepressant properties of cysteamine on hippocampal BDNF levels and behavioral despair in mice. Prog Neuropsychopharmacol Biol Psychiatry 32 (2008) 1590-1594
9. Tirouvanziam R., Conrad C.K., Bottiglieri T., Herzenberg L.A., Moss R.B., and Herzenberg L.A. High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis. Proc Natl Acad Sci U S A 103 (2006) 4628-4633
10. Xiao D., and Lin H.R. Effects of cysteamine-a somatostatin-inhibiting agent on serum growth hormone levels and growth in juvenile grass carp (Ctenopharyngodon idellus). Comp Biochem Physiol A Mol Integr Physiol 134 (2003) 93-99
11. Begley T.P., Kinsland C., and Strauss E. The biosynthesis of coenzyme A in bacteria. Vitam Horm 61 (2001) 157-171
12. Gerdes S.Y., Scholle M.D., D'Souza M., Bernal A., Baev M.V., Farrell M., et al. From genetic footprinting to antimicrobial drug targets: examples in cofactor biosynthetic pathways. J Bacteriol 184 (2005) 4555-4572
13. Bharara M.S., Kim C.H., Parkin S., and Atwood D.A. Synthesis and X-ray crystal structures of dinuclear hydrogen-bonded cadmium and lead 2-aminoethanethiolates. Polyhedron 24 (2005) 865-871
14. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
15. Jiang C., and Wu B. Molecular cloning and functional characterization of a novel decarboxylase from uncultured microorganisms. Biochem Biophys Res Commun 357 (2007) 421-426
16. Stanislawski J. Enzyme kinetics, version 1.5. (1991), Trinity Software, Fort Pierce, FL
17. Langer M., Gabor E.M., Liebeton K., Meurer G., Niehaus F., Schulze R., et al. Metagenomics: an inexhaustible access to nature's diversity. Biotechnol J 1 (2006) 815-821
18. Strauss E., Kinsland C., Ge Y., McLafferty F.W., and Begley T.P. Phosphopantothenoylcysteine synthetase from Escherichia coli. J Biol Chem 276 (2001) 13513-13516
19. Bosveld F., Rana A., Lemstra W., Kampinga H.H., and Sibon O.C. Drosophila phosphopantothenoylcysteine synthetase is required for tissue morphogenesis during oogenesis. BMC Res Notes 1 (2008) 75
20. Kupke T., Hernández-Acosta P., and Culiáñez-Macià F.A. 4′-Phosphopantetheine and coenzyme A biosynthesis in plants. J Biol Chem 278 (2003) 38229-38237