Genomic analysis identifies a transcription-factor binding motif regulating expression of the alpha C protein in group B Streptococcus

Microbial Pathogenesis

Volumn 46, Issue 6, 2009, Pages 315-320

  Klinzing, David C ^a   Madoff, Lawrence C ^a,b   Puopolo, Karen M ^a,b  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Group B Streptococcus; Streptococcus agalactiae; Transcriptional regulation

Indexed keywords

ALPHA C PROTEIN; BACTERIAL PROTEIN; DNA; TRANS ACTING FACTOR; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ALGORITHM; ARTICLE; BACTERIAL GENE; BINDING ASSAY; COMPUTER MODEL; CONTROLLED STUDY; DNA BINDING; DNA BINDING MOTIF; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENOME ANALYSIS; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN MOTIF; REGULATORY MECHANISM; REPORTER GENE; STREPTOCOCCUS AGALACTIAE; TRANSCRIPTION REGULATION;

AMINO ACID MOTIFS; ANTIGENS, SURFACE; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CONSERVED SEQUENCE; GENE EXPRESSION REGULATION, BACTERIAL; GENOMICS; HUMANS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS AGALACTIAE; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS SP. 'GROUP B';

EID: 67349241916     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2009.03.003     Document Type: Article

References (27)

1. Puopolo K.M., Madoff L.C., Baker C.J., and Group B. Streptococcal infection in neonates and young infants. In: Rose B.D. (Ed). UpToDate (2007), UpToDate, Waltham, MA
2. Liu G.Y., and Nizet V. Extracellular virulence factors of group B streptococci. Front Biosci 9 (2004) 1794-1802
3. Ferrieri P., Baker C.J., Hillier S.L., and Flores A.E. Diversity of surface protein expression in group B streptococcal colonizing & invasive isolates. Indian J Med Res Suppl. 119 (2004) 191-196
4. Persson E., Berg S., Bevanger L., Bergh K., Valsö-Lyng R., and Trollfors B. Characterisation of invasive group B streptococci based on investigation of surface proteins and genes encoding surface proteins. Clin Microbiol Infect 14 1 (2008) 66-73
5. Li J., Kasper D.L., Ausubel F.M., Rosner B., and Michel J.L. Inactivation of the alpha C protein antigen gene, bca, by a novel shuttle/suicide vector results in attenuation of virulence and immunity in group B Streptococcus. Proc Natl Acad Sci U S A 94 (1997) 13251-13256
6. Baron M.J., Filman D.J., Prophete G.A., Hogle J.M., and Madoff L.C. Identification of a glycosaminoglycan-binding region of the alpha C protein that mediates entry of group B streptococci into host cells. J Biol Chem 282 14 (2007) 10526-10536
7. Baron M.J., Bolduc G.R., Goldberg M.B., Auperin T.C., and Madoff L.C. Alpha C protein of group B Streptococcus binds host cell surface glycosaminoglycan and enters cells by an actin-dependent mechanism. J Biol Chem 279 (2004) 24714-24723
8. Bolduc G.R., Baron M.J., Gravecamp C., Lachenauer C.S., and Madoff L.C. The alpha C protein mediates internalization of group B Streptococcus within human cervical epithelial cells. Cell Microbiol 4 (2002) 751-758
9. Bolduc G.R., and Madoff L.C. The group B streptococcal alpha C protein binds alpha1beta1-integrin through a novel KTD motif that promotes internalization of GBS within human epithelial cells. Microbiology 153 (2007) 4039-4049
10. Gravekamp C., Rosner B., and Madoff L.C. Deletion of repeats in the alpha C protein enhances the pathogenicity of group B streptococci in immune mice. Infect Immun 66 (1998) 4347-4354
11. Madoff L.C., Michel J.L., Gong E.W., Kling D.E., and Kasper D.L. Group B streptococci escape host immunity by deletion of tandem repeat elements of the alpha C protein. Proc Natl Acad Sci U S A 93 (1996) 4131-4136
12. Poyart C., Lamy M.C., Boumaila C., Fiedler F., and Trieu-Cuot P. Regulation of d-alanyl-lipoteichoic acid biosynthesis in Streptococcus agalactiae involves a novel two-component regulatory system. J Bacteriol 183 21 (2001) 6324-6334
13. Puopolo K.M., and Madoff L.C. Upstream short sequence repeats regulate expression of the alpha C protein of group B Streptococcus. Mol Microbiol 50 (2003) 977-991
14. Gutekunst H., Eikmanns B.J., and Reinscheid D.J. Analysis of RogB-controlled virulence mechanisms and gene repression in Streptococcus agalactiae. Infect Immun 71 (2003) 5056-5064
15. Jiang S.M., Cieslewicz M.J., Kasper D.L., and Wessels M.R. Regulation of virulence by a two-component system in group B Streptococcus. J Bacteriol 187 (2005) 1105-1113
16. Lamy M.C., Zouine M., Fert J., Vergassola M., Couve E., Pellegrini E., et al. CovS/CovR of group B Streptococcus: a two-component global regulatory system involved in virulence. Mol Microbiol 54 (2004) 1250-1268
17. Spellerberg B., Rozdzinski E., Martin S., Weber-Heynemann J., and Lutticken R. rgf Encodes a novel two-component signal transduction system of Streptococcus agalactiae. Infect Immun 70 (2002) 2434-2440
18. Tettelin H., Masignani V., Cieslewicz M.J., Eisen J.A., Peterson S., Wessels M.R., et al. Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. Proc Natl Acad Sci U S A 99 (2002) 12391-12396
19. Tettelin H., Masignani V., Cieslewicz M.J., Donati C., Medini D., Ward N.L., et al. Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial "pan-genome". Proc Natl Acad Sci U S A 102 (2005) 13950-13955
20. Lachenauer C.S., Creti R., Michel J.L., and Madoff L.C. Mosaicism in the alpha-like protein genes of group B streptococci. Proc Natl Acad Sci U S A 97 (2000) 9630-9635
21. Kellis M., Patterson N., Birren B., Berger B., and Lander E.S. Methods in comparative genomics: genome correspondence, gene identification and regulatory motif discovery. J Comput Biol 11 (2004) 319-355
22. van Nimwegen E., Zavolan M., Rajewsky N., and Siggia E.D. Probabilistic clustering of sequences: inferring new bacterial regulons by comparative genomics. Proc Natl Acad Sci U S A 99 (2002) 7323-7328
23. Puopolo K.M., Hollingshead S.K., Carey V.J., and Madoff L.C. Tandem repeat deletion in the alpha C protein of group B Streptococcus is recA-independent. Infect Immun 69 (2001) 5037-5045
24. Samen U.M., Eikmanns B.J., and Reinscheid D.J. The transcriptional regulator RovS controls the attachment of Streptococcus agalactiae to human epithelial cells and the expression of virulence genes. Infect Immun 74 (2006) 5625-5635
25. Gravekamp C., Kasper D.L., Michel J.L., Kling D.E., Carey V., and Madoff L.C. Immunogenicity and protective efficacy of the alpha C protein of group B streptococci are inversely related to the number of repeats. Infect Immun 65 (1997) 5216-5221
26. Aldridge P.D., Gray M.A., Hirst B.H., and Anjam Khan C.M. Who's talking to whom? Epithelial-bacterial pathogen interactions. Mol Microbiol 55 3 (2005) 655-663
27. Dunny G.M., Lee L.N., and Leblanc D.J. Improved electroporation and cloning vector system for gram-positive bacteria. Appl Environ Microbiol 57 (1991) 1194-1201