MALDI-TOF mass spectrometry for quantitative gene expression analysis of acid responses in Staphylococcus aureus

Journal of Microbiological Methods

Volumn 78, Issue 1, 2009, Pages 86-93

  Rode, Tone Mari ^a,b   Berget, Ingunn ^b   Langsrud, Solveig ^a   Møretrø, Trond ^a   Holck, Askild ^a  

^a NOFIMA   (Norway)

^b NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

Author keywords

Acid stress; MALDI TOF MS; MassARRAY; Microarray; Staphylococcus aureus

Indexed keywords

BACTERIAL RNA; METAL ION; UREASE;

ACIDITY; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; GENE EXPRESSION; IONIZATION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROARRAY ANALYSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROKARYOTE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; STAPHYLOCOCCUS AUREUS; UPREGULATION;

ACIDS; GENE EXPRESSION REGULATION, BACTERIAL; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAPHYLOCOCCUS AUREUS;

PROKARYOTA; STAPHYLOCOCCUS AUREUS;

EID: 67349239824     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.05.006     Document Type: Article

References (16)

1. Alwine J.C., Kemp D.J., and Stark G.R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc. Natl. Acad. Sci. U.S.A. 74 (1977) 5350-5354
2. Beenken K.E., Dunman P.M., McAleese F., Macapagal D., Murphy E., Projan S.J., Blevins J.S., and Smeltzer M.S. Global gene expression in Staphylococcus aureus biofilms. J. Bacteriol. 186 (2004) 4665-4684
3. Bore E., Langsrud S., Langsrud O., Rode T.M., and Holck A. Acid-shock responses in Staphylococcus aureus investigated by global gene expression analysis. Microbiology 153 (2007) 2289-2303
4. Bowler L.D., Hubank M., and Spratt B.G. Representational difference analysis of cDNA for the detection of differential gene expression in bacteria: development using a model of iron-regulated gene expression in Neisseria meningitidis. Microbiology 145 Pt 12 (1999) 3529-3537
5. BμG@S, 2002. Microarray Protocols. RNA vs RNA: Labelling & Hybridisation. http://bugs.sgul.ac.uk/protocols/RNAvsRNA_hybridisation.PDF.
6. Cotter P.D., and Hill C. Surviving the acid test: responses of Gram-positive bacteria to low pH. Microbiol. Mol. Biol. Rev. 67 (2003) 429-453
7. Ding C., and Cantor C.R. A high-throughput gene expression analysis technique using competitive PCR and matrix-assisted laser desorption ionization time-of-flight MS. PNAS 100 (2003) 3059-3064
8. Frees D., Chastanet A., Qazi S., Sorensen K., Hill P., Msadek T., and Ingmer H. Clp ATPases are required for stress tolerance, intracellular replication and biofilm formation in Staphylococcus aureus. Mol. Microbiol. 54 (2004) 1445-1462
9. Heid C.A., Stevens J., Livak K.J., and Williams P.M. Real time quantitative PCR. Genome Res. 6 (1996) 986-994
10. Kappes R.M., Kempf B., and Bremer E. Three transport systems for the osmoprotectant glycine betaine operate in Bacillus subtilis: characterization of OpuD. J. Bacteriol. 178 (1996) 5071-5079
11. Langsrud Ø., Jørgensen K., Ofstad R., and Næs T. Analyzing designed experiments with multiple responses. J. Appl. Stat. 34 (2007) 1275-1296
12. Leushner J. MALDI TOF mass spectrometry: an emerging platform for genomics and diagnostics. Expert Rev. Mol. Diagn. 1 (2001) 11-18
13. Schena M., Shalon D., Davis R.W., and Brown P.O. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270 (1995) 467-470
14. Schmidt K.D., Schmidt-Rose T., Romling U., and Tummler B. Differential genome analysis of bacteria by genomic subtractive hybridization and pulsed field gel electrophoresis. Electrophoresis 19 (1998) 509-514
15. Velculescu V.E., Zhang L., Vogelstein B., and Kinzler K.W. Serial analysis of gene expression. Science 270 (1995) 484-487
16. Yang Y.H., and Speed T. Design issues for cDNA microarray experiments. Nat. Rev. Gen. 3 (2002) 579-588