A fully recombinant ELISA using in vivo biotinylated antibody fragments for the detection of potato leafroll virus

Journal of Virological Methods

Volumn 159, Issue 2, 2009, Pages 200-205

  Al Mrabeh, Ahmad ^a,b   Ziegler, Angelika ^a   Cowan, Graham ^a   Torrance, Lesley ^a  

^a SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

In vivo biotinylation; PLRV; Recombinant antibody; ScFv; Streptavidin; V3HCL

Indexed keywords

ALKALINE PHOSPHATASE; BIOTIN; HYBRID PROTEIN; IMMUNOGLOBULIN; REAGENT; RECOMBINANT ANTIBODY; STREPTAVIDIN; UNCLASSIFIED DRUG; V3HCL RECOMBINANT ANTIBODY;

ARTICLE; BIOTINYLATION; CONJUGATE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOASSAY; IMMUNOREACTIVITY; IN VIVO STUDY; NONHUMAN; PHAGE DISPLAY; POLEROVIRUS; POTATO; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION;

BIOTINYLATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOGLOBULIN FRAGMENTS; LUTEOVIRIDAE; PLANT DISEASES; SOLANUM TUBEROSUM; STAINING AND LABELING;

POTATO LEAFROLL VIRUS; SOLANUM TUBEROSUM;

EID: 67349214111     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.03.025     Document Type: Article

References (20)

1. Barat B., and Wu A.M. Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmatic reticulum. Biomol. Eng. 24 (2007) 283-291
2. Barker H., Webster K.D., Jolly C.A., Reavy B., Kumar A., and Mayo M.A. Enhancement of resistance to potato leaf roll virus multiplication in potato by combining the effects of host genes and transgenes. MPMI 7 (1994) 528-530
3. Bayer E.A., and Wilchek M. Introduction to avidin-biotin technology. In: Wilchek M., and Bayer E. (Eds). Methods in Enzymology. Vol 184: Avidin-Biotin Technology (1990), Academic Press, Inc. 5-13
4. Bulow L., and Linbladh C. Recombinant techniques in the preparation of protein conjugates. In: Aslam M., and Dent A. (Eds). Bioconjugation Protein Coupling Techniques for the Biomedical Science (1998), Macmillan Reference Ltd. 577-580
5. Cronan J.E. Biotinylation of proteins in vivo. A post-translational modification to label, purify, and study proteins. J. Biol. Chem. 265 (1990) 10327-11033
6. Harper K., Kerschbaumer R.J., Ziegler A., Macintosh S.M., Cowan G.H., Himmler G., Mayo M.A., and Torrance L. A scFv-alkaline phosphatase fusion protein which detects potato leaf roll luteovirus in plant extracts by ELISA. J. Virol. Methods 63 (1997) 237-242
7. Harper K., Toth R.L., Mayo M.A., and Torrance L. Properties of a panel of single chain variable fragments against potato leaf roll virus obtained from two phage display libraries. J. Virol. Methods 81 (1999) 159-168
8. Hermanson G.T. The avidin biotin systems. Bioconjugate Techniques (1996), Academic Press Inc. pp. 570-592
9. Kerschbaumer R.J., Hirschl S., Kaufmann A., Ibl M., Koenig R., and Himmler G. Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double sandwich enzyme-linked immunosorbant assay. Anal. Biochem. 249 (1997) 219-227
10. Krebber A., Bornhauser S., Burmester J., Honegger A., Willuda J., Bosshard H.R., and Plückthun A. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. J. Immunol. Methods 201 (1997) 35-55
11. Nissim A., Hoogenboom H.R., Tomlinson I.M., Flynn G., Midgeley C., Lane D., and Winter G. Antibody fragments from a single pot phage display library as immunochemical reagents. EMBO J. 13 (1994) 692-698
12. Orecchia M., Nolke G., Saldarelli P., Dell'Orco M., Uhde K., Martelli G., Fischer R., and Schillberg S. Generation and characterization of a functional recombinant antibody fragment that binds to the coat protein of Grapevine leafroll associated virus 3. Arch. Virol. 153 (2008) 1075-1084
13. Saldarelli P., Keller K., Dell'Orco M., Schots A., Elicio V., and Minafra A. Isolation of recombinant antibodies (scFvs) to grapevine virus B. J. Virol. Methods 124 (2005) 191-195
14. Schatz P.J. Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme-a 13 residue consensus peptide specifies biotinylation in Escherichia coli. Biotechnology 11 (1993) 1138-1143
15. Terrada E., Kerschbaumer R.J., Giunta G., Galeffi P., Himmler G., and Cambra M. Fully recombinant enzyme-linked immunosorbent assays using genetically engineered single-chain antibody fusion proteins for detection of citrus tristeza virus. Phytopathology 90 (2000) 1337-1344
16. Torrance L. Serological methods to detect plant viruses: production and use of monoclonal antibodies. In: Duncan J.M., and Torrance L. (Eds). Techniques for the Rapid Detection of Plant Pathogens (1992), Blackwell Scientific Publications, Oxford, United Kingdom 27-33
17. Toth R.L., Harper K., Mayo M.A., and Torrance L. Fusion proteins of single-chain variable fragments derived from phage display libraries are effective reagents for routine diagnosis of potato leaf roll virus infection in potato. Phytopathology 89 (1999) 1015-1021
18. Vaughan T.J., Williams A.J., Pritchard K., Osbourn J.K., Pope A.R., Earnshaw J.C., McCafferty J., Hodits R.A., Wilton J., and Johnson K.S. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat. Biotechnol. 14 (1996) 309-314
19. Warren D., Bjerner J., Paus E., Bormer O., and Nustad K. Use of an in vivo biotinylated single-chain antibody as a capture reagent in an immunometric assay to decrease the incidence of interference from heterophilic antibodies. Clin. Chem. 51 (2005) 830-838
20. Ziegler A., and Torrance L. Application of recombinant antibodies in plant pathology. Mol. Plant Pathol. 3 (2002) 401-407