Methods for sample labeling and meniscus determination in the fluorescence-detected analytical ultracentrifuge

Analytical Biochemistry

Volumn 390, Issue 2, 2009, Pages 218-220

  Bailey, Michael F ^a   Angley, Lauren M ^a   Perugini, Matthew A ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CENTRIFUGES; DISSOCIATION; ESCHERICHIA COLI; FLUORESCENCE;

ANALYTICAL ULTRACENTRIFUGE; DISSOCIATION CONSTANT; FLUORESCENCE DETECTION SYSTEM; FLUORESCENT DYES; HYDRODYNAMIC PROPERTIES; KLENOW FRAGMENT; LIGHT OIL;

BIOMOLECULES;

BACTERIAL DNA; BACTERIAL PROTEIN; FLUORESCENT DYE; OIL;

ARTICLE; CHEMICAL ANALYSIS; CHEMICAL LABELING; DISSOCIATION CONSTANT; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; GENE INTERACTION; MOLECULE; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PURIFICATION; SYNTHESIS; ULTRACENTRIFUGE;

ESCHERICHIA COLI;

EID: 67349212256     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.03.045     Document Type: Article

References (15)

1. Svedberg T., and Pederson K.O. The Ultracentrifuge (1940), Oxford University Press, Oxford, UK
2. Cao W., and Demeler B. Modeling analytical ultracentrifugation experiments with an adaptive space-time finite element solution for multicomponent reacting systems. Biophys. J. 95 (2008) 54-65
3. Demeler B. UltraScan: a comprehensive data analysis software package for analytical ultracentrifugation experiments. In: Scott D.J., Harding S.E., and Rowe A.J. (Eds). Modern Analytical Ultracentrifugation: Techniques and Methods (2005), Royal Society of Chemistry, London 210-229
4. Lebowitz J., Lewis M., and Schuck P. Modern analytical ultracentrifugation in protein science: a tutorial review. Prot. Sci. 11 (2002) 2067-2079
5. Schuck P. Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and Lamm equation modeling. Biophys. J. 78 (2000) 1606-1619
6. Schuck P., Perugini M.A., Gonzales N.R., Howlett G.J., and Schubert D. Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems. Biophys. J. 82 (2002) 1096-1111
7. Stafford W.F., and Sherwood P.J. Analysis of heterologous interacting systems by sedimentation velocity: curve fitting algorithms for estimation of sedimentation coefficients, equilibrium, and kinetic constants. Biophys. Chem. 108 (2004) 231-243
8. Laue T.M. Optical Systems of the XLA Ultracentrifuge [applications data note] (1996), Spinco division of Beckman Instruments, Palo Alto, CA
9. Cole J.L., and Hansen J.C. Analytical ultracentrifugation as a contemporary biomolecular research tool. J. Biomol. Tech. 10 (1999) 163-176
10. MacGregor I.K., Anderson A.L., and Laue T.M. Fluorescence detection for the XLI ultracentrifuge. Biophys. Chem. 108 (2004) 165-185
11. Burgess B.R., Dobson R.C.J., Bailey M.F., Atkinson S.C., Griffin M.D.W., Jameson G.B., Parker M.W., Gerrard J.A., and Perugini M.A. Structure and evolution of a novel dimeric enzyme from a clinically-important bacterial pathogen. J. Biol. Chem. 283 (2008) 27598-27603
12. Cole J.L., Lary J.W., Moody T.P., and Laue T.M. Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. Methods Cell Biol. 84 (2008) 143-179
13. Kroe R.R., and Laue T.M. NUTS and BOLTS: applications of fluorescence-detected sedimentation. Anal. Biochem. 390 (2009) 1-13
14. Bailey M.F., Van der Schans E.J., and Millar D.P. Dimerization of the Klenow fragment of Escherichia coli DNA polymerase I is linked to its mode of DNA binding. Biochemistry 46 (2007) 8085-8099
15. Joyce C.M., and Derbyshire V. Purification of E. coli DNA polymerase I and Klenow fragment. Methods Enzymol. 262 (1995) 3-13