NUTS and BOLTS: Applications of fluorescence-detected sedimentation

Analytical Biochemistry

Volumn 390, Issue 1, 2009, Pages 1-13

  Kroe, Rachel R ^a   Laue, Thomas M ^b  

^a BOEHRINGER INGELHEIM PHARMACEUTICALS INC   (United States)

^b UNIVERSITY OF NEW HAMPSHIRE   (United States)

Author keywords

Analytical ultracentrifugation; Fluorescence detected sedimentation; Green fluorescent protein

Indexed keywords

CENTRIFUGATION; FLUORESCENCE; MACROMOLECULES; NUTS (FASTENERS); SEDIMENTATION;

ANALYTICAL ULTRACENTRIFUGATION; ANALYTICAL ULTRACENTRIFUGE; COMPLEX MIXTURE; DILUTE SOLUTION; FLUORESCENCE DETECTOR; GREEN FLUORESCENT PROTEIN; HIGH AFFINITY; SOLUTION BEHAVIOR;

BOLTS;

ANALYTIC METHOD; ARTICLE; BIOLOGICAL ON LINE TRACER SEDIMENTATION; CELL LYSATE; FLUORESCENCE; HUMAN; MACROMOLECULE; NORMAL USE TRACER SEDIMENTATION; PRIORITY JOURNAL; SEDIMENTATION; SENSITIVITY ANALYSIS; ULTRACENTRIFUGE;

EID: 65649116623     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.11.033     Document Type: Article

References (25)

1. Cole J.L., Lary J.W., Moody T.P., and Laue T.M. Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium. In: Correia J.J., and Detrich H. (Eds). Methods in Cell Biology vol. 84 (2007), Elsevier, San Diego 143-179
2. Howlett G.J., Minton A.P., and Rivas G. Analytical ultracentrifugation for the study of protein association and assembly. Curr. Opin. Chem. Biol. 10 (2006) 430-436
3. T.M. Laue, Optical systems of the XLA ultracentrifuge, Applications Data Note for Spinco Division of Beckman Instruments, Palo Alto, CA, 1996.
4. MacGregor I.K., Anderson A.L., and Laue T.M. Fluorescence detection for the XLI ultracentrifuge. Biophys. Chem. 108 (2004) 165-185
5. Laue T.M., Austin J.B., and Rau D.A. A light intensity measurement system for the analytical ultracentrifuge. Prog. Colloid Polym. Sci. 131 (2006) 1-8
6. Crepeau R.H., Conrad R.H., and Edelstein S.J. UV laser scanning and fluorescence monitoring of analytical ultracentrifugation with an on-line computer system. Biophys. Chem. 5 (1976) 27-39
7. Schmidt B., Rappold W., Rosenbaum V., Fischer R., and Riesner D. A fluorescence detection system for the analytical ultracentrifuge and its application to proteins, nucleic acids, and viruses. Colloid Polym. Sci. 268 (1989) 45-54
8. Kingsbury J.S., Laue T.M., Klimtchuk E.S., Theberge R., Costello C.E., and Connors L.H. The modulation of transthyretin tetramer stability by cysteine 10 adducts and the drug diflunisal: direct analysis by fluorescence-detected analytical ultracentrifugation. J. Biol. Chem. 283 (2008) 11887-11896
9. Bothwell M.A., Howlett G.J., and Schachman H.K. A sedimentation equilibrium method for determining molecular weights of proteins with a tabletop high speed air turbine centrifuge. J. Biol. Chem. 253 (1978) 2073-2077
10. Darawshe S., Rivas G., and Minton A.P. Rapid and accurate microfractionation of the contents of small centrifuge tubes: application in the measurement of molecular weight of proteins via sedimentation equilibrium. Anal. Biochem. 209 (1993) 130-135
11. Schuck P. Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and Lamm equation modeling. Biophys. J. 78 (2000) 1606-1619
12. Stafford W.F., and Sherwood P.J. Analysis of heterologous interacting systems by sedimentation velocity: curve fitting algorithms for estimation of sedimentation coefficients, equilibrium, and kinetic constants. Biophys. Chem. 108 (2004) 231-243
13. Philo J.S. Improved methods for fitting sedimentation coefficient distributions derived by time-derivative techniques. Anal. Biochem. 354 (2006) 238-246
14. R. Kroe, Applications of fluorescence detected sedimentation, Ph.D. thesis, University of New Hampshire, 2005.
15. S. Bean, Determination of partial specific volume: using polyethylene glycol, ovalbumin, lysozyme, dextran, and green fluorescent protein, senior thesis, University of New Hampshire, 2006.
16. Ryan T.M., Howlett G.J., and Bailey M.F. Fluorescence detection of a lipid induced tetrameric intermediate in amyloid fibril formation by apolipoprotein C-II. J. Biol. Chem. 283 (2008) 35118-35128
17. Burgess B.R., Dobson R.C.J., Bailey M.F., Atkinson S.C., Griffin M.D.W., Jameson G.B., Parker M.W., Gerrard J.A., and Perugini M.A. Structure and evolution of a novel dimeric enzyme from a clinically important bacterial pathogen. J. Biol. Chem. 283 (2008) 27598-27603
18. Pauling L., Pressman D., and Campbell D.H. An experimental test of the framework theory of antigen-antibody precipitation. Science 98 (1943) 263-264
19. Rivas G., and Minton A.P. Tracer sedimentation equilibrium: a powerful tool for the quantitative characterization of macromolecular self- and hetero-association in solution. Biochem. Soc. Trans. 31 (2003) 1015-1019
20. Heidelberger M., and Kendall F.E. A quantitative theory of the precipitin reaction: II. A study of an azoprotein-antibody system. J. Exp. Med. 62 (1935) 467-483
21. Rowe A.J. The concentration dependence of transport processes: a general description applicable to the sedimentation, translational diffusion, and viscosity coefficients of macromolecular solutes. Biopolymers 16 (1977) 2595-2611
22. P.L. Altman, Blood and other body fluids, ASD Technical Report 61-199, Federation of American Societies for Experimental Biology, Washington, DC, 1961.
23. Johnston J.P., and Ogston A.G. A boundary anomaly found in the ultracentrifugal sedimentation of mixtures. Trans. Faraday Soc. 42 (1946) 789-799
24. Correia J.J., Johnson M.L., Weiss G.H., and Yphantis D.A. Numerical study of the Johnston-Ogston effect in two-component systems. Biophys. Chem. 5 (1976) 255-264
25. Stafford W.F. Analysis of reversibly interacting macromolecular systems by time derivative sedimentation velocity. In: Johnson M.L., and Ackers G.K. (Eds). Methods in Enzymology, vol. 233, Part C: Energetics of Biological Macromolecules (2000), Academic Press, San Diego 302-325