Real-time PCR for identification of Brucella spp.: A comparative study of IS711, bcsp31 and per target genes

Veterinary Microbiology

Volumn 137, Issue 1-2, 2009, Pages 156-164

  Bounaadja, Lotfi ^a,b   Albert, David ^c   Chénais, Benoît ^a   Hénault, Sylvie ^c   Zygmunt, Michel S ^d   Poliak, Sylvie ^b   Garin Bastuji, Bruno ^c  

^a UNIVERSITÉ DE NANTES   (France)

^b Centre Intersectoriel d'Addictologie Clinique Prémartine   (France)

^c Healthy Pigs Production and Experimentation Section   (France)

^d UR1282   (France)

Author keywords

bcsp31; Brucella; Genus specific identification; IS711; per; Real time PCR

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BIOTYPE; BRUCELLA; CONTROLLED STUDY; GENE TARGETING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SEROLOGY;

BRUCELLA; DNA, BACTERIAL; GENES, BACTERIAL; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

ANIMALIA; BRUCELLA; BRUCELLA MELITENSIS; BRUCELLA MELITENSIS BIOVAR ABORTUS; BRUCELLA MELITENSIS BIOVAR OVIS;

EID: 67349201006     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2008.12.023     Document Type: Article

References (33)

1. Al Dahouk S., Nöckler K., Scholz H.C., Pfeffer M., Neubauer H., and Tomaso H. Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp. Clin. Chem. Lab. Med. 45 (2007) 1464-1470
2. Baily G.G., Krahn J.B., Drasar B.S., and Stoker N.G. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hyg. 95 (1992) 271-275
3. Bogdanovich T., Skurnik M., Lübeck P.S., Ahrens P., and Hoorfar J. Validated 5' Nuclease PCR Assay for rapid identification of the genus Brucella. J. Clin. Microbiol. 42 (2004) 2261-2263
4. Bricker B.J., Ewalt D.R., MacMillan A.P., Foster G., and Brew S. Molecular characterization of Brucella strains isolated from marine mammals. J. Clin. Microbiol. 38 (2000) 1258-1262
5. Bricker B.J. PCR as a diagnostic tool for brucellosis. Vet. Microbiol. 90 (2002) 435-446
6. Cloeckaert A., Grayon M., and Grepinet O. An IS711 element downstream of the bp26 gene is a specific of Brucella spp. Isolated from marine mammals. Clin. Diagn. Lab. Immunol. 7 (2000) 835-839
7. Da Costa M., Guillou J.P., Garin-Bastuji B., Thiébaud M., and Dubray G. Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J. Appl. Bacteriol. 81 (1996) 267-275
8. Debeaumont C., Falconnet P.A., and Maurin M. Real-time PCR for detection of Brucella spp. DNA in human serum samples. Eur. J. Clin. Microbiol. Infect. Dis. 24 (2005) 842-845
9. Foster G., Osterman B.S., Godfroid J., Jacques I., and Cloeckaert A. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int. J. Syst. Evol. Microbiol. 57 (2007) 2688-2693
10. Garin-Bastuji B., Hummel N., Gerbier G., Cau C., Pouillot R., Da Costa M., and Fontaine J.J. Non specific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica O:9. Vet. Microbiol. 66 (1999) 223-233
11. Godfroid J., Saegerman C., Wellemans V., Walravens K., Letesson J.J., Tibor A., Mc Millan A., Spencer S., Sanna M., and Bakker D. How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet. Microbiol. 90 (2002) 461-477
12. Godfroid J., Cloeckaert A., Liautard J.P., Kohler S., Fretin D., Walravens K., Garin-Bastuji B., and Letesson J.J. From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet. Res. 36 (2005) 313-326
13. Halling S.M., Tatum F.M., and Bricker B.J. Sequence and characterization of an insertion sequence, IS711, from Brucella ovis. Gene 133 (1993) 123-127
14. Hénault S., Calvez D., Thiébaud M., Boulière M., and Garin-Bastuji B. Validation of a nested-PCR based on the IS6501/711 sequence for the detection of Brucella in animal samples. Proceedings of the Brucellosis 2000 International Research Conference (including the 53rd Brucellosis Research Conference). Nîmes, France (2000) 45
15. Herman L., and De Ridder H. Identification of Brucella spp. by using the polymerase chain reaction. Appl. Environ. Microbiol. 58 (1992) 2099-2101
16. Lübeck P.S., Skurnik M., Ahrens P., and Hoorfar J. A multiplex PCR-detection assay for Yersinia enterocolitica serotype O:9 and Brucella spp. based on the perosamine synthetase gene. Application to Brucella diagnostics. Adv. Exp. Med. Biol. 529 (2003) 451-453
17. Matar G., Khneisser I.A., and Abdelnoor A.M. Rapid laboratory diagnosis of human brucellosis by PCR analysis of target sequence on the 31-kilodalton Brucella antigen DNA. J. Clin. Microbiol. 34 (1996) 477-478
18. Navarro E., Fernandez J.A., Escribano J., and Solera J. PCR assay for diagnosis of human Brucellosis. J. Clin. Microbiol. 37 (1999) 1654-1655
19. Navarro E., Segura J.C., Castaño M.J., and Solera J. Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis. Clin. Infect. Dis. 42 (2006) 1266-1273
20. Newby D.T., Hadfield T.L., and Roberto F.F. Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 59-exonuclease, and hybridization probe assays. Appl. Environ. Microbiol. 69 (2003) 4753-4759
21. Nielsen K., Smith P., Widdison J., Gall D., Kelly L., Kelly W., and Nicoletti P. Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O157:H7. Vet. Microbiol. 100 (2004) 25-30
22. O'Leary S., Sheahan M., and Sweeney T. Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Res. Vet. Sci. 81 (2006) 170-176
23. Ouahrani S., Michaux S., Sri Widada J., Bourg G., Tournebize R., Ramuz M., and Liautard J.P. Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the number of IS6501 copies. J. Gen. Microbiol. 139 (1993) 3265-3273
24. Pappas G., Papadimitriou P., Akritidis N., Christou L., and Tsianos E.V. The new global map of human brucellosis. Lancet Infect. Dis. 6 (2006) 91-99
25. Probert W.S., Schrader K.N., Khuong N.Y., Bystrom S.L., and Graves M.H. Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis. J. Clin. Microbiol. 42 (2004) 1290-1293
26. Queipo-Ortuño M.I., Colmenero J.D., Reguera J.M., García-Ordoñez M.A., Pachón M.E., Gonzalez M., and Morata P. Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples. Clin. Microbiol. Infect. 11 (2005) 713-718
27. Redkar R., Rose S., Bricker B., and DelVecchio V. Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis. Mol. Cell. Probes 15 (2001) 43-52
28. Romero C., Gamazo C., Pardo M., and Lopez-Goni I. Specific detection of Brucella DNA by PCR. J. Clin. Microbiol. 33 (1995) 615-617
29. Scholz H.C., Hubalek Z., Sedlácek I., Vergnaud G., Tomaso H., Al Dahouk S., Melzer F., Kämpfer P., Neubauer H., Cloeckaert A., Maquart M., Zygmunt M.S., Whatmore A.M., Falsen E., Bahn P., Göllner C., Pfeffer M., Huber B., Busse H.J., and Nöckler K. Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int. J. Syst. Evol. Microbiol. 58 (2008) 375-382
30. Velasco J., Romero C., López-Goñi I., Leiva J., Díaz R., and Moriyon I. Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. nov., a new species with a closer relationship to Brucella spp. Int. J. Syst. Bacteriol. 48 (1998) 759-768
31. Wrathall A.E., Broughton E.S., Gill K.P., and Goldsmith G.P. Serological reactions to Brucella species in British pigs. Vet. Res. 132 (1993) 449-454
32. Yanagi M., and Yamasato K. Phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16S rRNA gene using PCR and DNA sequencer. FEMS Microbiol. Lett. 107 (1993) 115-120
33. Young E.J. Serologic diagnosis of human brucellosis: analysis of 214 cases by agglutination tests and review of the literature. Rev. Infect. Dis. 13 (1991) 359-372