Development and validation of real-time one-step reverse transcription-PCR for the detection and typing of dengue viruses

Journal of Clinical Virology

Volumn 45, Issue 1, 2009, Pages 61-66

  Leparc Goffart, Isabelle ^a   Baragatti, Meili ^a   Temmam, Sarah ^b   Tuiskunen, Anne ^a   Moureau, Gregory ^b   Charrel, Rémi ^b   de Lamballerie, Xavier ^b  

^a SERVICE DE SANTÉ DES ARMÉES   (France)

^b Aix Marseille University IRD 190 Inserm 1207   (France)

Author keywords

Dengue virus; Diagnosis; Real time RT PCR; Standardization; Validation

Indexed keywords

ARTICLE; DENGUE VIRUS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; EARLY DIAGNOSIS; FLAVIVIRUS; GENE SEQUENCE; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SEROTYPE; UNINDEXED SEQUENCE; VALIDATION PROCESS; VIRUS DETECTION; VIRUS STRAIN; VIRUS TYPING;

EID: 67349150737     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2009.02.010     Document Type: Article

References (12)

1. World Health Organization. Dengue and dengue haemorrhagic fever. Fact sheet; 2002, 117.
2. Kao C.-L., King C.-C., Chao D.-Y., Wu H.-L., and Chang G.-J. Laboratory diagnosis of dengue virus infection: current and future perspectives in clinical diagnosis and public health. J Microbiol Immunol Infect 38 (2005) 5-16
3. Callahan J.D., Wu S.-J.L., Dion-Schultz A., Mangold B.E., Peruski L.F., Watts D.M., et al. Development and evaluation of serotype- and group-specific fluorogenic reverse transcriptase PCR (TaqMan) assays for dengue virus. J Clin Microbiol 39 (2001) 4119-4124
4. Drosten C., Göttig S., Schilling S., Asper M., Panning M., Schmitz H., et al. Rapid detection and quantification of RNA of ebola and marburg viruses, lassa virus, Crimean-congo hemorrhagic fever virus, rift valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. J Clin Microbiol 40 (2002) 2323-2330
5. Ito M., Takasaki T., Yamada K.-I., Nerome R., Tajima S., and Kurane I. Development and evaluation of fluorogenic taqman reverse transcriptase PCR assays for detection of dengue virus types 1 to 4. J Clin Microbiol 42 (2004) 5935-5937
6. Johnson B.W., Russell B.J., and Lanciotti R.S. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. J Clin Microbiol 43 (2005) 4977-4983
7. Kong Y.Y., Thay C.H., Tin T.C., and Devi S. Rapid detection, serotyping and quantitation of dengue viruses by taqman real-time one-step RT-PCR. J Virol Methods 138 (2006) 123-130
8. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Res 25 (1997) 4876-4882
9. Moureau G., Temmam S., Gonzalez J.P., Charrel R.N., Grard G., and De Lamballerie X. A real-time RT-PCR method for the universal detection and identification of flaviviruses. Vector Borne Zoonotic Dis 8 (2008) 1-11
10. International Cooperation on harmonisation of technical requirements for registration of pharmaceuticals for human use. Validation of analytical procedures: text and methodology Q2 (R1).
11. Van der Schaar H.M., Rust M.J., Waarts B.-L., van der Ende-Metselaar, Kuhn R.J., Wilschut J., et al. Characterization of the early events in dengue virus cell entry by biochemical assays and single-virus tracking. J Virol 81 (2007) 12019-12028
12. Domingues R.B., Kuster G.W., Onuki-Castro F.L., Souza V.A., Levi J.E., and Pannuti C.S. Involvement of the central nervous system in patients with dengue virus infection. J Neurol Sci 267 (2008) 36-40