Reverse transcription loop-mediated isothermal amplification for rapid and sensitive detection of nervous necrosis virus in groupers

Journal of Virological Methods

Volumn 159, Issue 2, 2009, Pages 206-210

  Sung, Chia Hsuan ^a   Lu, Jenn Kan ^b  

^a FISHERIES RESEARCH INSTITUTE   (Taiwan)

^b NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

Author keywords

Epinephelus coioides; Epinephelus lanceolatus; Nervous necrosis virus; NNV; Reverse transcription loop mediated isothermal amplification; RT LAMP

Indexed keywords

COAT PROTEIN; PRIMER RNA; VIRUS RNA;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EXTRACTION; FISH; GENE AMPLIFICATION; GENE SEQUENCE; INTERMETHOD COMPARISON; NERVOUS NECROSIS VIRUS; NODAVIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REACTION TIME; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TEMPERATURE; VIRUS DETECTION;

ANIMALS; DNA PRIMERS; FISH DISEASES; FISHES; NODAVIRIDAE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; RNA VIRUS INFECTIONS; SENSITIVITY AND SPECIFICITY; TEMPERATURE; TIME FACTORS;

ATLANTIC HALIBUT NODAVIRUS; EPINEPHELINAE; EPINEPHELUS COIOIDES; PROMICROPS LANCEOLATUS; STRIPED JACK NERVOUS NECROSIS VIRUS;

EID: 67349112928     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.03.022     Document Type: Article

References (19)

1. Arimoto M., Mushiake K., Mizuta Y., Nakai T., Muroga K., and Furusawa T. Detection of striped jack nervous necrosis virus (SJNNV) by enzyme-linked immunosorbent assay (ELISA). Fish Pathol. 27 (1992) 191-195
2. Chi S., Lo C.F., Kou G., Chang P., Peng S., and Chen S. Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus akaara (Temminck & Schlegel). J. Fish Dis. 20 (1997) 185-193
3. Chi S.C., Lin S.C., Su H.M., and Hu W.W. Temperature effect on nervous necrosis virus infection in grouper cell line and in grouper larvae. Virus Res. 63 (1999) 107-114
4. Chi S.C., Shieh J.R., and Lin S.J. Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Dis. Aquat. Organ. 55 (2003) 221-228
5. Comps M., Pepin J., and Bonami J. Purification and characterization of two fish encephalitis viruses (FEV) infecting Lates calcarifer and Dicentrarchus labrax. Aquaculture 123 (1994) 1-10
6. Comps M., Trindade M., and Delsert C. Investigation of fish encephalitis viruses (FEV)expression in marine fishes using DIG-labelled probes. Aquaculture 143 (1996) 113-121
7. Dalla Valle L., Toffolo V., Lamprecht M., Maltese C., Bovo G., Belvedere P., and Colombo L. Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR. Vet. Microbiol. 110 (2005) 167-179
8. Kiatpathomchai W., Jareonram W., Jitrapakdee S., and Flegel T.W. Rapid and sensitive detection of Taura syndrome virus by reverse transcription loop-mediated isothermal amplification. J. Virol. Methods 146 (2007) 125-128
9. Le Breton A., Grisez L., Sweetman J., and Ollevier F. Viral nervous necrosis (VNN) associated with mass mortalities in cage-reared sea bass, Dicentrarchus labrax L. J. Fish Dis. 20 (1997) 145-151
10. Millard P.J., Bickerstaff L.E., LaPatra S.E., and Kim C.H. Detection of infectious haematopoietic necrosis virus and infectious salmon anaemia virus by molecular padlock amplification. J. Fish Dis. 29 (2006) 201-213
11. Mori K., Nakai T., Muroga K., Arimoto M., Mushiake K., and Furusawa I. Properties of a new virus belonging to nodaviridae found in larval striped jack (Pseudocaranx dentex) with nervous necrosis. Virology 187 (1992) 368-371
12. Mori Y., Nagamine K., Tomita N., and Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289 (2001) 150-154
13. Nishizawa T., Mori K., Furuhashi M., Nakai T., Furusawa I., and Muroga K. Comparison of the coat protein genes of five fish nodaviruses, the causative agents of viral nervous necrosis in marine fish. J. Gen. Virol. 76 Pt 7 (1995) 1563-1569
14. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., and Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28 (2000) E63
15. Savan R., Kono T., Itami T., and Sakai M. Loop-mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens. J. Fish Dis. 28 (2005) 573-581
16. Shieh J.R., and Chi S.C. Production of monoclonal antibodies against grouper nervous necrosis virus (GNNV) and development of an antigen capture ELISA. Dis. Aquat. Organ. 63 (2005) 53-60
17. Soliman H., and El-Matbouli M. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS). Vet. Microbiol. 114 (2006) 205-213
18. Starkey W.G., Millar R.M., Jenkins M.E., Ireland J.H., Muir K.F., and Richards R.H. Detection of piscine nodaviruses by real-time nucleic acid sequence based amplification (NASBA). Dis. Aquat. Organ. 59 (2004) 93-100
19. Tomita N., Mori Y., Kanda H., and Notomi T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Protoc. 3 (2008) 877-882