Purification and characterization of antibacterial proteins from granular hemocytes of Indian mud crab, Scylla serrata

Acta Biochimica Polonica

Volumn 56, Issue 1, 2009, Pages 71-82

  Yedery, Roshan Dinesh ^a   Reddy, Kudumula Venkata Rami ^a  

^a NATIONAL INSTITUTE FOR RESEARCH IN REPRODUCTIVE HEALTH   (India)

Author keywords

Antibacterial protein; Crab; Hemocytes; Innate immunity; Scylla serrata

Indexed keywords

DECAPODA (CRUSTACEA); INVERTEBRATA; NEGIBACTERIA; POSIBACTERIA; SCYLLA; SCYLLA SERRATA;

EID: 67249136865     PISSN: 0001527X     EISSN: 1734154X     Source Type: Journal    
DOI: 10.18388/abp.2009_2518     Document Type: Article

References (44)

1. Andreu D, Rivas L (1998) Animal antimicrobial peptides: an overview. Biopolymers 47: 415-433.
2. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of dye binding. Anal Biochem 72: 248-254.
3. Brogden KA (2005) Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol 3: 238-250
4. Casteels P, Ampe C, Jacobs F, Tempst P (1993) Functional and chemical characterization of Hymenoptaecin, an antibacterial polypeptide that is infection induced in the honeybee (Apis mellifera). J Biol Chem 268: 7044-7054.
5. Chattopadhyay T, Chatterjee B (1997) Further biochemical and biophysical characterisation of scyllin, Scylla serrata hemolymph lectin. Biochem Mol Biol Int 42: 183-191.
6. Cole AM (2005) Antimicrobial peptide microbicides targeting HIV. Protein Pept Lett 12: 41-47.
7. Cooper EL (1985) Overview of humoral factors in invertebrates. Dev Comp Immunol 9: 577-583.
8. Hall SH, Hamil KG, French FS (2002) Host defense proteins of the male reproductive tract. J Androl 23: 585-597.
9. Harder J, Bartels J, Christophers E, Schröder J-M (2001) Isolation and characterization of human β-defensin-3, a novel human inducible peptide antibiotic. J Biol Chem 276: 5707-5713.
10. Hoq MI, Seraj MU, Chowdhury S (2003) Isolation and characterization of antimicrobial peptides from the mud crab, Scylla serrata. Pakistan J Biol Sci 6: 1345-1353.
11. Huang WS, Wang KJ, Yang M, Cai JJ, Li SJ, Wang GZ (2006) Purification and part characterization of a novel antibacterial protein scygonadin, isolated from the seminal plasma of mud crab, Scylla serrata (Forskal). J Exp Mar Boil Ecol 339: 37-42.
12. Hudson DA, Lester RJG (1994) Parasites and symbionts of wild mud crabs, Scylla serrata (Forskal) of potential significance in aquaculture. Aquaculture 120: 183-199.
13. Hughes AL, Yeager M (1997) Coordinated amino acid changes in the evolution of mammalian defensins. J Mol Evol 44: 675-682.
14. Huttner KM, Brezinski-Caliguri DJ, Mahoney MM, Diamond G (1998) Antimicrobial peptide expression is developmentally regulated in the ovine gastrointestinal tract. J Nutrition 128: 297S-299S.
15. Iijima N, Tanimoto N, Emoto Y, Morita Y, Uematsu K, Murakami T, Nakai T (2003) Purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, Chrysophrys major. Eur J Biochem 270: 675-686.
16. Imjongjirak C, Amparyup P, Tassanakajon A, Sittipraneed S (2007) Antilipopolysaccharide factor (ALF) of mud crab Scylla paramamosain: molecular cloning, genomic organization and the antimicrobial activity of its synthetic LPS binding domain. Mol Immunol 44: 3195-3203.
17. Iwanaga S, Lee BL (2005) Recent advances in the innate immunity of invertebrate animals. Biochem Mol Biol 38: 128-150.
18. Janeway Jr CA, Medzhitov R (2002) Innate immune recognition. Annu Rev Immunol 20: 197-216.
19. Jiravanichpaisal P, Lee BK, Soderhall K (2006) Cell-mediated immunity in arthropods: hematopoiesis, coagulation, melanization and opsonization. Immunobiology 211: 213-236.
20. Kurtz J (2005) Specific memory within innate immune systems. Trends Immunol 26: 186-192.
21. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
22. Lee SY, Soderhall K (2002) Early events in crustacean innate immunity. Fish Shellfish Immunol 12: 421-437.
23. Lehrer RI, Ganz T (1999) Antimicrobial peptides in mammalian and insect host defense. Curr Opin Immunol 11: 23-27.
24. Marchini D, Manetti AGO, Rosetto M, Bernini LF, Telford JL, Baldari CT, Dallai R (1995) cDNA sequence and expression of the ceratotoxin gene encoding an antibacterial sex-specific peptide from the medfly Ceratitis capitata (Diptera). J Biol Chem 270: 6199-6204.
25. Mitta G, Vandenbulcke F, Noel T, Romestand B, Beauvillain JC, Salzet M, Roch P (2000) Differential distribution and defense involvement of antimicrobial peptides in mussel. J Cell Sci 113: 2759-2769.
26. Miyata T, Tokunaga F, Yoneya T, Yoshikawa K, Iwanaga S, Niwa M, Takao T, Shimonishi Y (1989) Antimicrobial peptides, isolated from horseshoe crab hemocytes, tachyplesin II and polyphemusins I and II: chemical structures and biological activity. J Biochem (Tokyo) 106: 663-668.
27. Morita T, Ohtsubo S, Nakamura T, Tanaka S, Iwanaga S, Ohashi K, Niwa M. (1985) Isolation and biological activities of limulus anticoagulant (anti-LPS factor) which interacts with lipopolysaccharide (LPS). J Biochem 97: 1611-1620.
28. Murakami T, Niwa M, Tokunaga F, Miyata T, Iwanaga S (1991) Direct virus inactivation of tachyplesin-I and its isopeptides from horseshoe crab hemocytes. Chemotherapy 37: 327-334.
29. Quayle AJ, Porter EM, Nussbaum AA, Wang YM, Brabec C, Yip KP, Mok SC (1998) Gene expression, immunolocalization, and secretion of human defensin-5 in human female reproductive tract. Am J Pathol 152: 1247-1258.
30. Reddy KVR, Clara A, Gupta SM, Yedery RD (2004a) Evaluation of antimicrobial peptide, Nisin as a safe vaginal contraceptive agent in rabbits: in vitro and in vivo studies. Reproduction 128: 126-137.
31. Reddy KVR, Yedery RD, Clara A (2004b) Antimicrobial peptides: premises and promises. Int J Antimicrobial Agents 24: 536-547.
32. Relf JM, Chisholm JRS, Kemp GD, Smith VJ (1999) Purification and characterization of a cysteine-rich 11.5 kDa antibacterial protein from the granular hemocytes of the shore crab, Carcinus maenas. Eur J Biochem 264: 350-357.
33. Samakovlis C, Kylsten P, Kimbrell DA, Engström A, Hultmark D (1991) The andropin gene and its product, a male-specific antibacterial peptide in Drosophila melanogaster. EMBO J 10: 163-169.
34. Samonte GPB, Agbayani RF (1992) Pond culture of mud crab (Scylla serrata) and economic analysis, SEAFDEC. Asian Aquaculture 14: 3-5.
35. Schnapp D, Kemp GD, Smith VJ (1996) Purification and characterization of a proline rich anti-bacterial peptide, with sequence similarity to bactenecin 7, from the hemocytes of the shore crab, Carcinus maenas. Eur J Biochem 240: 532-539.
36. Smith VJ, Ratcliffe NA (1980) Defensive reactions of the shore crab, Carcinus maenas. In vivo haemocytic and histopathological responses to injected bacteria. J InvPathol 35: 65-74.
37. Soderhall K, Smith VJ (1983) Separation of hemocytes of Carcinus maenas and other decapod crustaceans and phenoloxidase distribution. Dev Comp Immunol 7: 229-239.
38. Spear GT, StJohn E, Zariffard MR (2007) Bacterial vaginosis and human immunodeficiency virus infection. AIDS Res Therapy 4: 25.
39. Towle DW, Smith CM (2006) Gene discovery in Carcinus maenas and Homarus americanus via expressed sequence tags. Integ Comp Biol 46: 912-918.
40. Wang KJ, Huang WS, Yang M, Chen HY, Bo J, Li SJ, Wang GZ (2007) A male-specific expression gene, encodes a novel anionic antimicrobial peptide, scygonadin, in Scylla serrata. Mol Immunol 44: 1961-1968.
41. Wira CR, Fahey JV (2004) The innate immune system: gatekeeper to the female reproductive tract. Immunology 111: 13-15.
42. Yedery RD, Reddy KVR (2005) Antimicrobial peptides as microbicidal contraceptives: prophecies for prophylactics. Eur J Contracept Reprod Health Care 10: 32-42.
43. Zasloff M (1987) Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci USA 84: 5449-5453.
44. Zhao C, Nguyen T, Liu L, Shamova O, Brogden K, Lehrer RI (1999) Differential expression of caprine β-defensins in digestive and respiratory tissues. Infect Immun 11: 6221-6224.