Purification and characterization of a proline-rich antibacterial peptide, with sequence similarity to bactenecin-7, from the haemocytes of the shore crab, Carcinus maenas

European Journal of Biochemistry

Volumn 240, Issue 3, 1996, Pages 532-539

  Schnapp, Denni ^a   Kemp, Graham D ^a   Smith, Valerie J ^a  

^a UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

Antimicrobial peptide; Bactenecin; Carcinus maenas; Cathelicidin; Crustacea

Indexed keywords

ANTIINFECTIVE AGENT; BACTENECIN;

ANIMAL CELL; ANTIBACTERIAL ACTIVITY; ARTICLE; BLOOD CELL; CATTLE; CONTROLLED STUDY; CRAB; EXTRACTION; GEL FILTRATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HOST RESISTANCE; MOLECULAR WEIGHT; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMPHIBIA; ANIMALIA; BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; CARCINUS MAENAS; CHELICERATA; CRUSTACEA; DECAPODA (CRUSTACEA); GRAPSIDAE; HEXAPODA; MAMMALIA; NEGIBACTERIA; POSIBACTERIA;

EID: 0029762649     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0532h.x     Document Type: Article

References (42)

1. Azumi, K., Yokosawa, H. & Ishii, S. (1990) Halocyamines; novel antimicrobial tetrapeptide-like substances isolated from the hemocytes of the solitary ascidian, Halocynthia roretzi, Biochemistry 29, 159-165.
2. Boman, H. G. (1994) Assaying antimicrobial activity, Ciba Found. Symp. 186, 54-61.
3. Boman, H. G. (1995) Peptide antibiotics and their role in innate immunity, Annu. Rev. Immunol. 13, 61-92.
4. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Anal. Biochem. 72, 248-254.
5. Bulet, P., Dimarcq, J. L., Hetru, C., Lagueux, C., Charlet, M. Heggy, G., Van Drosselaer, A. & Hoffmann, J. A. (1993) A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution. J. Biol. Chem. 268, 14893-14897.
6. Casteels, P. Ampe, C., Jacobs, F., Vaeck, M. & Tempst, P. (1989) Apidaecins: antibacterial peptides from honeybees. EMBO J. 8, 2387-2391.
7. Casteels, P., Ampe, C., Riviere, L., Damme, J. V., Elicone, C., Fleming, M., Jacobs, F. & Tempst, P. (1990) Isolation and characterisation of abaecin, a major antibacterial response peptide in the honeybee (Apis mellifera), J. Biol. Chem. 268, 7044-7054.
8. Casteels, P. & Tempst, P. (1994) Apidaccin-type peptide antibiotics function through a non-pore forming mechanism involving stereospecificity. Biochem. Biophys. Res. Commun. 199, 339-345.
9. Chalk, R., Townson, H., Natori, S., Desmond, H. & Ham, P. J. (1994) Purification of an insect defensin from the mosquito, Aedes aegypti, Insect Biochem. Mol. Biol. 24, 403-410.
10. Chernysh, S., Cociancich, S., Brind, J. P., Hetru, C. & Bulet, P. (1996) The inducible antibacterial peptides of the hemipteran insect Palomena prasina: Identification of a unique family of proline-rich peptides and of a novel insect defensin, J. Insect. Physiol. 42, 81-89.
11. Chin, G. J. & Marx, J. (eds) (1994) Resistance to antibiotics. Science 264, 359-393.
12. Chisholm, J. R. S. (1993) Antibacterial activity in the blood cells of Carcinus maenas (L) and other marine crustaceans. PhD thesis, University of St Andrews.
13. Chisholm, J. R. S. & Smith, V. J. (1992) Antibacterial activity in the haemocytes of the shore crab Carcinus maenas, J. Mar. Biol. Ass. UK 72, 529-542.
14. Chisholm, J. R. S. & Smith, V. J. (1995) Compulsion of antibacterial activity in the haemocytes of different crustacean species. Comp. Biochem. Physiol. 110A, 39-45.
15. Cociancich, S., Bulet, P., Hetru, C. & Hoffmann, J. A. (1994a) The inducible antibacterial peptides of insects. Parasitol. Today 10, 132-139.
16. Cociancich, S., Dupont, A., Hegy, G., Lanot, R., Holder, F., Hetru, C., Hoffmann, J. A. & Bulet, P. (1994b) Novel inducible antibacterial peptides from a hemipteran insect, the sap-sucking bug Pyrrhocoris apterus, Biochem. J. 300, 567-575.
17. Cruciani, R. A., Barker, J. L., Zasloff, M., Chen, H. C. & Colamonici, O. (1991) Antibiotic magainins exert cytolytic activity against transformed cells through channel formation. Proc. Natl Acad. Sci. USA 88, 3792-3796.
18. Daher, K. A., Selsted, M. E. & Lehrer, R. I. (1986) Direct inactivation of viruses by human granulocyte defensin. J. Virol. 60, 1068-1074.
19. Findlay, C. & Smith, V. J. (1995) Antimicrobial factors in solitary ascidians, Fish Shellfish Immunol. 5, 645-658.
20. Frank, R. W., Gennaro, R., Schneider, K., Przybylski, M. & Romeo, D. (1990) Amino acid sequences of 2 proline-rich bactenecins antimicrobial peptides of bovine neutrophils, J. Biol. Chem. 265, 18871-18874.
21. Fulks, W. & Main, K. L. (1992) Introduction, in Diseases of cultured penaeid shrimp in Asia and the United States (Fulks, W. & Main, K. L., eds) pp. 3-33. Oceanic Institute, Honolulu.
22. Ganz, T., Selsted, M. E., Szklarek, D., Harwig, S. S. L., Daher, K., Bainton, D. F. & Lehrer, R. I. (1985) Defensins, Natural peptide antibiotics of human neutrophils. J. Clin. Invest. 76, 1427-1435.
23. Genetics Computer Group Inc. (1994) Program manual for the Wisconsin package version 8, Sept. 1994. Genetics Computer Group Inc., Madison, Wis.
24. Gennaro, R., Skerlavaj, B. & Romeo, D. (1989) Purification, composition and activity of two bactenecins; antibacterial peptides of bovine neutrophils, Infect. Immun. 57, 3142-3146.
25. Hara, S. & Yamakawa, M. (1995) A novel antibacterial peptide family isolated from the silkworm Bombyx mori, Biochem. J. 310, 651-656.
26. Hoffmann, J. A. (1995) Innate immunity in insects. Curr. Opin. Immunol. 7, 4-10.
27. Hoffmann, J. & Hetru, C. (1992) Insect detensins: inducible antibacterial peptides, Immunol. Today 13, 411-415.
28. Hultmark, D., Engström, Ä., Andersson, K., Steiner, H., Bennich, H. & Boman, H. G. (1983) Insect immunity. Attacins, a family of antibacterial proteins from Hyalophora cecropia. EMBO J. 2, 571-576.
29. Iwanaga, S., Muta, T., Shigenaga, T., Seki, N., Kawano, K., Katsu, T. & Kawabata, S. (1994) Structure-function relationships of tachyplesins and their analogues, Ciba Found. Symp. 186, 160-175.
30. Lehrer, R. L. Rosenman, M., Harwig, S. S. L., Jackson, R. & Eisenhauer P. (1991) Ultrasensitive assays for endogenous antimicrobial polypeptides. J. Immunol. Methods 137, 167-173.
31. Levashina, E. A., Ohresser, S., Bulet, P., Reichhart, J. M., Hetru, C. & Hoffmann, J. A. (1995) Metchnikowin, a novel immune-inducible proline-rieh peptide from Drosophila with antibacterial and antifungal properties, Eur. J. Biochem. 233, 694-700.
32. Nakamura, T., Furunaka, H., Miyat, T., Tokunaga, F., Muta, T. & Iwanaga, S. (1988) Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus). J. Biol Chem. 263, 16709-16713.
33. Roe, S. (1989) Separations based on structure, in Protein purification methods, a practical approach (Harris, E. L. V. & Angal, S., eds) pp. 175-243, IRL Press, Oxford.
34. Rosenberry, B. (1991) World shrimp farming, Academic Press, San Diego CA.
35. Saito, T., Kawabata, S., Shigenaga, T., Takayenoki, Y., Cho, J., Nakajima, H., Hirata, M. & Iwanaga, S. (1995) A novel big defensin identified in horseshoe crab hemocytes: isolation, amino acid sequence and antibacterial activity. J. Biochem. (Tokyo) 117, 1131-1137.
36. Schägger, H. & von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range of 1 to 100 kDa, Anal. Biochem. 166, 368-379.
37. Schnapp, D. & Smith, V. J. (1996) Antibacterial proteins in the hemocytes of the shore crab, Carcinus maenas, in Modulators of the immune response (Stolen, J., Zelikoff, J., Twerdok, L., Bayne, C. J., Fletcher, T. C., Hefti, E., Secombes, C. J., Anderson, D. P., Kattari, S., eds) pp. 189-196, SOS Publications, Fair Haven NJ.
38. Skerlavaj, B., Romeo, D. & Gennaro, R. (1990) Rapid membrane permeabilization and inhibition of vital functions of gram-negative bacteria by bactenecins. Infect. Immun. 58, 3724-3730.
39. Söderhäll, K., Cerenius, L. & Johansson, M. W. (1994) The prophenoloxidase activating system and its role in invertebrate defense. Ann. NY Acad. Sci. 712, 155-161.
40. Steiner, H., Hultmark, D., Engström, Å., Bennich, H. & Boman, H. G. (1981) Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature 292, 246-248.
41. Sugumaran, M., Hennigan, B., Semensi, V., Mitchell, W. & Rivera, T. (1988) Differential mechanism of oxidation of N-acetyl dopamine and N-acetyl norepinephrine by cuticular phenoloxidase from Sarcophaga bullata, Arch. Insect Biochem. Physiol. 8, 229-241.
42. Zanetti, M., Gennaro, R. & Romeo, D. (1995) Cathelicidins: a novel protein family with a common proregion and a variable C-terminal antimicrobial domain, FEBS Lett. 374, 1-5.