Comparison of two enzyme immunoassay for detection of Clostridium difficile toxin A and toxin B;Clostridium difficile Korean Source

Korean Journal of Laboratory Medicine

Volumn 29, Issue 2, 2009, Pages 122-126

  Shin, Bo Moon ^a   Yoo, Soo Jin ^a   Oh, Hye Jun ^a  

^a Inje University College of Medicine   (South Korea)

Author keywords

Clostridium difficile; Enzyme immunoassay; PCR; Toxin A; Toxin B

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; ENTEROTOXIN; FLUORESCENT DYE; TCDA PROTEIN, CLOSTRIDIUM DIFFICILE; TOXB PROTEIN, CLOSTRIDIUM DIFFICILE;

ANALYTICAL EQUIPMENT; ARTICLE; CHEMISTRY; CLOSTRIDIUM DIFFICILE; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY;

BACTERIAL PROTEINS; BACTERIAL TOXINS; CLOSTRIDIUM DIFFICILE; ENTEROTOXINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FECES; FLUORESCENT DYES; HUMANS; REAGENT KITS, DIAGNOSTIC;

EID: 67249103183     PISSN: 15986535     EISSN: None     Source Type: Journal    
DOI: 10.3343/kjlm.2009.29.2.122     Document Type: Article

References (27)

1. Wilkins TD and Lyerly DM. Clostridium difficile testing: after 20 years, still challenging. J Clin Microbiol 2003;41:531-4.
2. Musher DM, Manhas A, Jain P, Nuila F, Waqar A, Logan N, et al. Detection of Clostridium difficile toxin: comparison of enzyme immunoassay results with results obtained by cytotoxicity assay. J Clin Microbiol 2007;45:2737-9.
3. Staneck JL, Weckbach LS, Allen SD, Siders JA, Gilligan PH, Coppitt G, et al. Multicenter evaluation of four methods for Clostridium difficile detection: immunoCard C. difficile, cytotoxin assay, culture and latex agglutination. J Clin Microbiol 1996;34:2718-21.
4. Peterson LR, Olson MM, Shanholtzer CJ, Gerding DN. Results of a prospective, 18-month clinical evaluation of culture, cytotoxin testing, and culturerette brand (CDT) latex testing in the diagnosis of Clostridium diffcile-associted diarrhea. Diag Microbiol Infect Dis 1988;10:85-91.
5. Lyerly DM, Neville LM, Evans DT, Fill J, Allen S, Greene W, et al. Multicenter evaluation of the Clostridium difficile TOX A/B test. J Clin Microbiol 1998;36:184-90.
6. Fedorko D, Engler HD, O'Shaughnessy EM, Williams EC, Reicheldereer CJ, Smith WI Jr. Evaluation of two rapid assays for detection of Clostridium difficile toxin A in stool specimens. J Clin Microbiol 1999;37:3044-7.
7. Kang JO, Chae JD, Eom JI, Han D, Park PW, Park IK, et al. Comparison of Clostridium difficile toxin A immunoassay with cytotoxicity assay. Korean J Clin Microbiol 2000:3;43-7. (Korean Source. Clostridium difficile Korean Source A Korean Source 2000;3:43-7.)
8. Lee SH and Pai CH. Clinical significance of VIDAS Clostridium difficile Toxin A immunoassay. Korean J Clin Pathol 1996;16:563-9. (Korean Source. VIDASKorean Source Clostridium difficile Toxin A Korean Source 1996;16:563-9.)
9. Rupnik, M, Kato N, Grabnar M, Kato H. New types of toxin A-negative, toxin B-Positive strains among Clostridium difficile isolates from Asia. J Clin Microbiol 2003;41:1118-25.
10. Samra Z, Talmor S, Bahar J. High prevalence of toxin A-negative toxin B-positive Clostridium difficile in hospitalized patients with gastrointestinal disease. Diagn Microbiol Infect Dis 2002;43:189-92.
11. Drudy D, Harnedy N, Fanning S, O'Mahony R, Kyne L. Isolation and characterisation of toxin A-negative, toxin B-positive Clostridium difficile in Dublin, Ireland. Clin Microbiol Infect 2007;13:298-304.
12. Shin BM and Kuak EY. Characterization of a toxin A-negative, toxin B-positive variant strain of Clostridium difficile. Korean J Lab Med 2006;26:27-31. (Korean Source A Korean Source B Korean Source Clostridium difficile Korean Source 2006;26:27-31.)
13. Kim H, Riley TV, Kim M, Kim CK, Yong D, Lee K, et al. Increasing prevalence of toxin A-negative, toxin B-positive isolates of Clostridium difficile in Korea: impact on laboratory diagnosis. J Clin Microbiol 2008;46:1116-7.
14. Shin BM, Kuak EY, Yoo SJ, Shin WC, Yoo HM. Emerging toxin AB+ variant strains of Clostridium difficile responsible for pseudomembranous colitis at a tertiary care hospital. Diagn Microbiol Infect Dis 2008;60:333-7.
15. Shin BM, Kuak EY, Yoo HM, Kim EC, Lee K, Kang KO, et al. Multicentre study of the prevalence of toxigenic Clostridium difficile in Korea: results of a retrospective study 2000-2005. J Med Microbiol 2008;57:697-701.
16. Shin BM and Lee EJ. Comparison of Toxin A enzyme linked fluorescence assay and latex agglutination based on Clostridium difficile culture and Toxin A and B PCR assay. Korean J Clin Microbiol 2005; 8:130-5. (Korean Source. Clostridium difficile Korean Source Toxin A, B PCR Korean Source Toxin A Korean Source 2005;8:130-5.)
17. Kato H, Kato N, Watanabe K, Iwai N, Nakamura H, Yamamoto T, et al. Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR. J Clin Microbiol 1998;36:2178-82.
18. Kang JO, Shin BM, Han D, Choi TY. Evaluation of the VIDAS CDAB kits for the detection of the Clostridium difficile toxin A and B. Korean J Clin Microbiol 2008;11:107-11. (Korean Source. Clostridium difficile Korean Source AKorean Source BKorean SourceVIDAS CDAB Korean Source. Korean Source 2008;11:107-11.)
19. Yoo SJ, Kang JO, Oh HJ, Shin BM. Comparison of two enzyme immunoassays for Clostridium difficile Toxin A. Korean J Lab Med 2006;26:408-11. (Korean Source. Clostridium difficile Korean SourceA Korean Source 2006;26:408-11.)
20. Barbut F, Delmee M, Brazier JS, Petit JC, Poxton IR, Rupnik M, et al. A European survey of diagnostic methods and testing protocols for Clostridium difficile. Clin Microbiol Infect 2003;9:989-96.
21. Turgeon DK, Novicki TJ, Quick J, Carlson L, Miller P, Ulness B, et al. Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay. J Clin Microbiol 2003;41:667-70.
22. Reyes RC, John MA, Ayotte DL, Covacich A, Milburn S, Hussain Z. Performance of TechLab C.DIFF QUIK CHEK and TechLab C.DIFFICILE TOX A/B II for the detection of Clostridium difficile in stool samples. Diagn Microbiol Infect Dis 2007;59:33-7.
23. van den Berg RJ, Bruijnesteijn van Coppernraet LS, Gerritsen HJ, Endtz HP, van der Vorm ER, Kuijper EJ. Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients. J Clin Microbiol 2005;43:5338-40.
24. Aldeen WE, Bingham M, Aiderzada A, Kucera J, Jense S, Carroll KC. Comparison of the TOX A/B test to a cell culture cytotoxicity assay for the detection of Clostridium difficile in stools. Diagn Microbiol Infect Dis 2000;36:211-3.
25. Ticehurst JR, Aird DZ, Dam LM, Borek AP, Hargrove JT, Carroll KC. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J Clin Microbiol 2006;44:1145-9.
26. Delmee M, Van Broeck J, Simon A, Janssens M, Avesani V. Laboratory diagnosis of Clostridium difficile associated diarrhoea: a plea for culture. J Med Microbiol 2005;54:187-91.
27. Harris AD, Samore MH, Lipsitch M, Kaye KS, Perencevich E, Carmeli Y. Control-group selection importance in studies of antimicrobial resistance: examples applied to Pseudomonas aeruginosa, Enterococci, and Escherichia coli. Clin Infect Dis 2002;15:1558-63.