The mitochondrial protein translocation motor: Structural conservation between the human and yeast tim14/pam18- tim16/pam16 co-chaperones

International Journal of Molecular Sciences

Volumn 10, Issue 5, 2009, Pages 2041-2053

  Elsner, Shira ^a   Simian, Dana ^a   Iosefson, Ohad ^a   Marom, Milit ^a   Azem, Abdussalam ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

MtHsp70; Tim14 (also known as Pam18); Tim16 (also known as Pam16); Translocation motor

Indexed keywords

CARRIER PROTEIN; HEAT SHOCK PROTEIN 70; HETERODIMER; MITOCHONDRIAL PROTEIN; PRESEQUENCE TRANSLOCASE ASSOCIATED PROTEIN IMPORT MOTOR 16; PRESEQUENCE TRANSLOCASE ASSOCIATED PROTEIN IMPORT MOTOR 18; TRANSLOCASE PROTEIN TIM14; TRANSLOCASE PROTEIN TIM16; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; COMPLEX FORMATION; ENZYME ACTIVITY; GENE TRANSLOCATION; GENETIC CONSERVATION; HUMAN; HYDROLYSIS; IN VITRO STUDY; NONHUMAN; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN STRUCTURE; PURIFICATION; YEAST;

MTHSP70; TIM14 (ALSO KNOWN AS PAM18); TIM16 (ALSO KNOWN AS PAM16); TRANSLOCATION MOTOR;

HSP70 HEAT-SHOCK PROTEINS; HUMANS; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIA; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIAL MEMBRANES; MITOCHONDRIAL PROTEINS; MITOCHONDRIAL PROTON-TRANSLOCATING ATPASES; PROTEIN STRUCTURE, SECONDARY; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 67049108831     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms10052041     Document Type: Article

References (34)

1. Rapaport, D. How does the TOM complex mediate insertion of precursor proteins into the mitochondrial outer membrane? J. Cell Biol. 2005, 171, 419-423.
2. Mokranjac, D.; Neupert, W. Protein import into mitochondria. Biochem. Soc. Trans. 2005, 33, 1019-1023.
3. Pfanner, N.; Chacinska, A. The mitochondrial import machinery: preprotein-conducting channels with binding sites for presequences. Biochim. Biophys. Acta 2002, 1592, 15-24.
4. Koehler, C.M. New developments in mitochondrial assembly. Annu. Rev. Cell Dev. Biol. 2004, 20, 309-335.
5. Meinecke, M.; Wagner, R.; Kovermann, P.; Guiard, B.; Mick, D.U.; Hutu, D.P.; Voos, W.; Truscott, K.N.; Chacinska, A.; Pfanner, N.; Rehling, P. Tim50 maintains the permeability barrier of the mitochondrial inner membrane. Science 2006, 312, 1523-1526.
6. van der Laan, M.; Rissler, M.; Rehling, P. Mitochondrial preprotein translocases as dynamic molecular machines. FEMS Yeast Res. 2006, 6, 849-861.
7. Stojanovski, D; Rissler, M.; Pfanner, N.; Meisinger ,C. Mitochondrial morphology and protein import-A tight connection? Biochim. Biophys. Acta 2006, 1763, 414-421.
8. Matouschek, A.; Pfanner, N.; Voos, W. Protein unfolding by mitochondria. The Hsp70 import motor. EMBO Rep. 2000, 1, 404-410.
9. Horst, M.; Azem, A.; Schatz, G.; Glick, B.S. What is the driving force for protein import into mitochondria? Biochim. Biophys. Acta 1997, 1318, 71-78.
10. Bomer, U.; Meijer, M.; Maarse, A.C.; Honlinger, A.; Dekker, P.J.; Pfanner, N.; Rassow, J. Multiple interactions of components mediating preprotein translocation across the inner mitochondrial membrane. EMBO J. 1997, 16, 2205-2216.
11. Truscott, K.N.; Voos, W.; Frazier, A.E.; Lind, M.; Li, Y.; Geissler, A.; Dudek, J.; Muller, H.; Sickmann, A.; Meyer, H.E.; Meisinger, C.; Guiard, B.; Rehling, P.; Pfanner, N. A J-protein is an essential subunit of the presequence translocase-associated protein import motor of mitochondria. J. Cell Biol. 2003, 163, 707-713.
12. Moro, F.; Sirrenberg, C.; Schneider, H.C.; Neupert, W.; Brunner, M. The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix. EMBO J. 1999, 18, 3667-3675.
13. Slutsky-Leiderman, O.; Marom, M.; Iosefson, O.; Levy, R.; Maoz, S.; Azem, A. The interplay between components of the mitochondrial protein translocation motor studied using purified components. J. Biol. Chem. 2007, 282, 33935-33942.
14. D'Silva, P.D.; Schilke, B.; Walter, W.; Andrew, A.; Craig, E.A. J protein cochaperone of the mitochondrial inner membrane required for protein import into the mitochondrial matrix. Proc. Nat. Acad. Sci. USA 2003, 100, 13839-13844.
15. Mokranjac, D.; Sichting, M.; Neupert, W.; Hell, K. Tim14, a novel key component of the import motor of the TIM23 protein translocase of mitochondria. EMBO J. 2003, 22, 4945-4956.
16. D'Silva, P.R.; Schilke, B.; Walter, W.; Craig, E.A. Role of Pam16's degenerate J domain in protein import across the mitochondrial inner membrane. Proc. Natl. Acad. Sci. USA 2005, 102, 12419-12424.
17. Frazier, A.E.; Dudek, J.; Guiard, B.; Voos, W.; Li, Y.; Lind, M.; Meisinger, C.; Geissler, A.; Sickmann, A.; Meyer, H.E.; Bilanchone, V.; Cumsky, MG.; Truscott, K.N.; Pfanner, N.; Rehling, P. Pam16 has an essential role in the mitochondrial protein import motor. Nat. Struct. Mol. Biol. 2004, 11, 226-233.
18. Kozany, C.; Mokranjac, D.; Sichting, M.; Neupert, W.; Hell, K. The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase. Nat. Struct. Mol. Biol. 2004, 11, 234-241.
19. Li, Y.; Dudek, J.; Guiard, B.; Pfanner, N.; Rehling, P.; Voos, W. The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18. J. Biol. Chem. 2004, 279, 38047-38054.
20. Mokranjac, D.; Bourenkov, G.; Hell, K.; Neupert, W.; Groll, M. Structure and function of Tim14 and Tim16, the J and J-like components of the mitochondrial protein import motor. EMBO J. 2006, 25, 4675-4685.
21. Davey, K.M.; Parboosingh, J.S.; McLeod, D.R.; Chan. A.; Casey, R.; Ferreira, P.; Snyder, F.F.; Bridge, P.J.; Bernier, F.P. Mutation of DNAJC19, a human homologue of yeast inner mitochondrial membrane co-chaperones, causes DCMA syndrome, a novel autosomal recessive Barth syndrome-like condition. J. Med. Genet. 2006, 43, 385-393.
22. Jubinsky, P.T.; Messer, A.; Bender, J.; Morris, R.E.; Ciraolo, G.M.; Witte, D.P.; Hawley, R.G.; Short, M.K. Identification and characterization of Magmas, a novel mitochondria-associated protein involved in granulocyte- macrophage colony-stimulating factor signal transduction. Exp. Hematol. 2001, 29, 1392-1402.
23. Jubinsky, P.T; Short, M.K.; Mutema, G.; Morris, R.E.; Ciraolo, G.M.; Li, M. Magmas expression in neoplastic human prostate. J. Mol. Histol. 2005, 36, 69-75.
24. Jubinsky, P.T.; Short, M.K.; Mutema, G.; Witte, D.P. Developmental expression of Magmas in murine tissues and its co-expression with the GM-CSF receptor. J. Histochem. Cytochem. 2003, 51, 585-596.
25. Peng, J.; Huang, C.H.; Short, M.K.; Jubinsky, P.T. Magmas gene structure and evolution. Silico Biol. 2005, 5, 251-263.
26. Iosefson, O.; Levy, R.; Marom, M.; Slutsky-Leiderman, O.; Azem, A. The Pam18/Tim14-Pam16/Tim16 complex of the mitochondrial translocation motor: The formation of a stable complex from marginally stable proteins. Protein Sci. 2007, 16, 316-322.
27. Neupert, W.; Herrmann, J.M. Translocation of proteins into mitochondria. Annu. Rev. Biochem 2007, 76, 723-749.
28. Kang, P.J.; Ostermann, J.; Shilling, J.; Neupert, W.; Craig, E.A.; Pfanner, N. Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor proteins. Nature 1990, 348, 137-143.
29. Westermann, B.; Prip-Buus, C.; Neupert, W.; Schwarz, E. The role of the GrpE homologue, Mge1p, in mediating protein import and protein folding in mitochondria. EMBO J. 1995, 14, 3452-3460.
30. Laloraya, S.; Gambill, B.D.; Craig, E.A. A role for a eukaryotic GrpE-related protein, Mge1p, in protein translocation. Proc. Natl. Acad. Sci. USA 1994, 91, 6481-6485.
31. Bolliger, L.; Deloche, O.; Glick, B.S.; Georgopoulos, C.; Jeno, P.; Kronidou, N.; Horst, M.; Morishima, N.; Schatz, G. A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability. EMBO J. 1994, 13, 1998-2006.
32. Mokranjac, D.; Sichting, M.; Popov-Celeketic, D.; Berg, A.; Hell, K.; Neupert, W. The import motor of the yeast mitochondrial TIM23 preprotein translocase contains two different J proteins, Tim14 and Mdj2. J. Biol. Chem. 2005, 280, 31608-31614.
33. Bonshtien, A.L.; Weiss, C.; Vitlin, A.; Niv, A.; Lorimer, G.H.; Azem, A. Significance of the N-terminal domain for the function of chloroplast cpn20 chaperonin. J. Biol. Chem. 2007, 282, 4463-4469.
34. Weiss, C.; Niv, A.; Azem, A. Two-step purification of mitochondrial Hsp70, Ssc1p, using Mge1(His)(6) immobilized on Ni-agarose. Protein Expr. Purif. 2002, 24, 268-273.