메뉴 건너뛰기




Volumn 331, Issue 2, 2004, Pages 401-403

An improvement of the site-directed mutagenesis Method by combination of megaprimer, one-side PCR and DpnI treatment

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY;

EID: 3242707573     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.04.019     Document Type: Article
Times cited : (27)

References (10)
  • 1
    • 0036425270 scopus 로고    scopus 로고
    • Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris
    • Sinclair G., Choy F.Y.M. Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast, Pichia pastoris. Prot. Expr. Purif. 26:2002;96-105
    • (2002) Prot. Expr. Purif. , vol.26 , pp. 96-105
    • Sinclair, G.1    Choy, F.Y.M.2
  • 2
    • 0037473220 scopus 로고    scopus 로고
    • Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels
    • Eric M.S., Henry A.L. Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels. J. Neurosci. Methods. 124:2003;75-81
    • (2003) J. Neurosci. Methods , vol.124 , pp. 75-81
    • Eric, M.S.1    Henry, A.L.2
  • 3
    • 0031574019 scopus 로고    scopus 로고
    • Approaches to DNA mutagenesis: An overview
    • Michael L.M., Brian H.R. Approaches to DNA mutagenesis: an overview. Anal. Biochem. 254:1997;157-178
    • (1997) Anal. Biochem. , vol.254 , pp. 157-178
    • Michael, L.M.1    Brian, H.R.2
  • 4
    • 0024346633 scopus 로고
    • Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR)
    • Kammann M., Laufs J., Schell J., Gronenborn B. Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR). Nucleic Acid Res. 17:1989;5404
    • (1989) Nucleic Acid Res. , vol.17 , pp. 5404
    • Kammann, M.1    Laufs, J.2    Schell, J.3    Gronenborn, B.4
  • 5
    • 0030743251 scopus 로고    scopus 로고
    • Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method
    • Song-Hua K., Edwin L.M. Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method. Nucleic Acid Res. 25:1997;3371-3372
    • (1997) Nucleic Acid Res. , vol.25 , pp. 3371-3372
    • Song-Hua, K.1    Edwin, L.M.2
  • 7
    • 0037098946 scopus 로고    scopus 로고
    • Site-directed mutagenesis by the megaprimer PCR method: Variation on a theme for simultaneous introduction of multiple mutations
    • Sebastiana A., Maria Carmela B.D.P. Site-directed mutagenesis by the megaprimer PCR method: variation on a theme for simultaneous introduction of multiple mutations. Anal. Biochem. 306:2002;346-349
    • (2002) Anal. Biochem. , vol.306 , pp. 346-349
    • Sebastiana, A.1    Maria Carmela, B.D.P.2
  • 8
    • 0029144643 scopus 로고
    • A one-step polymerase chain reaction site-directed mutagenesis method for large gene-cassettes with high efficiency yield and fidelity
    • Michael L.M., Brian H.R. A one-step polymerase chain reaction site-directed mutagenesis method for large gene-cassettes with high efficiency yield and fidelity. Anal. Biochem. 230:1995;167-172
    • (1995) Anal. Biochem. , vol.230 , pp. 167-172
    • Michael, L.M.1    Brian, H.R.2
  • 9
    • 0030608942 scopus 로고    scopus 로고
    • Site-directed mutagenesis: A two-step method using PCR and Dpn1
    • Li S., Wilkinson M.F. Site-directed mutagenesis: A two-step method using PCR and Dpn1. Biotechniques. 23:1997;588-590
    • (1997) Biotechniques , vol.23 , pp. 588-590
    • Li, S.1    Wilkinson, M.F.2
  • 10
    • 0038167027 scopus 로고    scopus 로고
    • Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA
    • Avinash R.S., Sandhya S.V. Site-directed mutagenesis using a single mutagenic oligonucleotide and DpnI digestion of template DNA. Anal. Biochem. 319:2003;335-336
    • (2003) Anal. Biochem. , vol.319 , pp. 335-336
    • Avinash, R.S.1    Sandhya, S.V.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.