Genetic diversity of the low-level vancomycin resistance gene vanC-2/vanC-3 and identification of a novel vanC subtype (vanC-4) in enterococcus casseliflavus

Microbial Drug Resistance

Volumn 15, Issue 1, 2009, Pages 1-9

  Watanabe, Shojiro ^a   Kobayashi, Nobumichi ^a   Quiñones, Dianelys ^b   Hayakawa, Satoshi ^c   Nagashima, Shigeo ^a   Uehara, Nobuyuki ^a   Watanabe, Naoki ^a  

^a SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b INSTITUTO DE MEDICINA TROPICAL PEDRO KOURÍ   (Cuba)

^c FUJITA HEALTH UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIUM ISOLATE; ENTEROCOCCUS CASSELIFLAVUS; GENE CLUSTER; GENE SEQUENCE; GENETIC VARIABILITY; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; VANC 2 GENE; VANC 3 GENE; VANC 4 GENE;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BACTERIAL PROTON-TRANSLOCATING ATPASES; DNA PRIMERS; ENTEROCOCCUS; GENOTYPE; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; PEPTIDE SYNTHASES; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; VANCOMYCIN; VANCOMYCIN RESISTANCE;

ENTEROCOCCUS CASSELIFLAVUS;

EID: 66749134056     PISSN: 10766294     EISSN: None     Source Type: Journal    
DOI: 10.1089/mdr.2009.0856     Document Type: Article

References (32)

1. Alam, M.M., N. Kobayashi, M. Ishino, A. Sumi, K. Kobayashi, N. Uehara, and N. Watanabe. 2005. Detection of a novel aph(2″″) allele (aph[2″″]-Ie) conferring high-level gentamicin resistance and a spectinomycin resistance gene ant(9)-Ia (aad 9) in clinical isolates of enterococci. Microb. Drug Resist. 11:239-247.
2. Arias, C.A., P. Courvalin, and P.E. Reynolds. 2000. vanC cluster of vancomycin-resistant Enterococcus gallinarum BM4174. Antimicrob. Agents Chemother. 44:1660-1666.
3. Barbier, N., P. Saulnier, E. Chachaty, S. Dumontier, and A. Andremont. 1996. Random amplified polymorphic DNA typing versus pulsed-field gel electrophoresis for epidemi-ological typing of vancomycin-resistant enterococci. J. Clin. Microbiol. 34:1096-1099.
4. Courvalin, P. 2006. Vancomycin resistance in gram-positive cocci. Clin. Infect. Dis. 42:S25-S34.
5. Dahl, K.H., G.S. Simonsen, O. Olsvik, and A. Sundsfjord. 1999. Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci. Antimicrob. Agents Chemother. 43:1105-1110.
6. Descheemaeker, P., C. Lammens, B. Pot, P. Vandamme, and H. Goossens. 1997. Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large geno-mic DNA fragments for identification of enterococci important in human medicine. Int. J. Syst. Bacteriol. 47:555-561.
7. Dutka-Malen, S., C. Molinas, M. Arthur, and P. Courvalin. 1992. Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine:D-alanine ligase-related protein necessary for vancomycin resistance. Gene 112:53-58.
8. Dutka-Malen, S., S. Evers, and P. Courvalin. 1995. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol. 33:24-27.
9. Dutta, I., and P.E. Reynolds. 2002. Biochemical and genetic characterization of the vanC-2 vancomycin resistance gene cluster of Enterococcus casseliflavus ATCC 25788. Antimicrob. Agents Chemother. 46:3125-3132.
10. Dutta, I., and P.E. Reynolds. 2003. The vanC-3 vancomycin resistance gene cluster of Enterococcus flavescens CCM 439. J. Antimicrob. Chemother. 51:703-706.
11. Endtz, H.P., N. van den Braak, A. van Belkum, J.A. Kluytmans, J.G. Koeleman, L. Spanjaard, A. Voss, A.J. Weersink, C.M. Vandenbroucke-Grauls, A.G. Buiting, A. van Duin, and H.A. Verbrugh. 1997. Fecal carriage of vancomycin-resistant enterococci in hospitalized patients and those living in the community in The Netherlands. J. Clin. Microbiol. 35:3026-3031.
12. Gold, H.S., S. Unal, E. Cercenado, C. Thauvin-Eliopoulos, G.M. Eliopoulos, C.B. Wennersten, and R.C. Moellering, Jr. 1993. A gene conferring resistance to vancomycin but not teicoplanin in isolates of Enterococcus faecalis and Enterococcus faecium demonstrates homology with vanB, vanA, and vanC genes of enterococci. Antimicrob. Agents Che-mother. 37:1604-1609.
13. Healy, V.L., I.S. Park, and C.T. Walsh. 1998. Active-site mutants of the VanC2 D-alanyl-D-serine ligase, characteris- tic of one vancomycin-resistant bacterial phenotype, revert towards wild-type D-alanyl-D-alanine ligases. Chem. Biol. 5:197-207.
14. Homan, W.L., D. Tribe, S. Poznanski, M. Li, G. Hogg, E. Spalburg, J.D. van Embden, and R.J. Willems. 2002. Mul-tilocus sequence typing scheme for Enterococcus faecium. J. Clin. Microbiol. 40:1963-1971.
15. Leclercq, R., S. Dutka-Malen, J. Duval, and P. Courvalin. 1992. Vancomycin resistance gene vanC is specific to Enterococcus gallinarum. Antimicrob. Agents Chemother. 36:2005- 2008.
16. Murase, T., Y. Mito, K. Otsuki, R. Suzuki, and S. Yamai. 2002. Nucleotide substitutions in vanC-2 gene of Enterococcus casseliflavus isolates obtained from chickens. Epidemiol. Infect. 129:421-424.
17. Murray, B.E. 1998. Diversity among multidrug-resistant enterococci. Emerg. Infect. Dis. 4:37-47.
18. Naser, S., F.L. Thompson, B. Hoste, D. Gevers, K. Vande-meulebroecke, I. Cleenwerck, C.C. Thompson, M. Van-canneyt, and J. Swings. 2005. Phylogeny and identification of enterococci by atpA gene sequence analysis. J. Clin. Mi-crobiol. 43:2224-2230.
19. Naser, S.M., M. Vancanneyt, B. Hoste, C. Snauwaert, K. Vandemeulebroecke, and J. Swings. 2006. Reclassification of Enterococcus flavescens Pompei et al. 1992 as a later synonym of Enterococcus casseliflavus (ex Vaughan et al., 1979) Collins et al. 1984 and Enterococcus saccharominimus Vancanneyt et al. 2004 as a later synonym of Enterococcus italicus Fortina et al. 2004. Int. J. Syst. Evol. Microbiol. 56:413- 416.
20. Navarro, F., and P. Courvalin. 1994. Analysis of genes encoding D-alanine-D-alanine ligase-related enzymes in Enterococcus casseliflavus and Enterococcus flavescens. Anti-microb. Agents Chemother. 38:1788-1793.
21. National Committee for Clinical Laboratory Standards. Approved standards. 1993. M7-A3. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aer-obically, third edition. National Committee for Clinical Laboratory Standards. Approved standards, Villanova, PA.
22. Nishimoto, Y., N. Kobayashi, M.M. Alam, M. Ishino, N. Uehara, and N. Watanabe. 2005. Analysis of the prevalence of tetracycline resistance genes in clinical isolates of En-terococcus faecalis and Enterococcus faecium in a Japanese hospital. Microb. Drug Resist. 11:146-153.
23. Park, I.S., C.H. Lin, and C.T. Walsh. 1997. Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase. Proc. Natl. Acad. Sci. USA 94:10040- 10044.
24. Patel, R., J.R. Uhl, P. Kohner, M.K. Hopkins, and F.R. Cockerill III. 1997. Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2=3 genes in enterococci. J. Clin. Microbiol. 35:703-707.
25. Patel, R., J.R. Uhl, P. Kohner, M.K. Hopkins, J.M. Steck-elberg, B. Kline, and F.R. Cockerill III. 1998. DNA sequence variation within vanA, vanB, vanC-1, and vanC-2/3 genes of clinical Enterococcus isolates. Antimicrob. Agents Chemother. 42:202-205.
26. Patel, R., K.E. Piper, M.S. Rouse, J.M. Steckelberg, J.R. Uhl, P. Kohner, M.K. Hopkins, F.R. Cockerill III, and B.C. Kline. 1998. Determination of 16S rRNA sequences of enterococci and application to species identification of non-motile Enterococcus gallinarum isolates. J. Clin. Microbiol. 36: 3399-3407.
27. Petersen, A., and A. Dalsgaard. 2003. Species composition and antimicrobial resistance genes of Enterococcus spp, isolated from integrated and traditional fish farms in Thailand. Environ. Microbiol. 5:395-402.
28. Reid, K.C., F.R. Cockerill III, and R. Patel. 2001. Clinical and epidemiological features of Enterococcus casseliflavus= flavescens and Enterococcus gallinarum bacteremia: a report of 20 cases. Clin. Infect. Dis. 32:1540-1546.
29. Reynolds, P.E., H.A. Snaith, A.J. Maguire, S. Dutka-Malen, and P. Courvalin. 1994. Analysis of peptidoglycan precursors in vancomycin-resistant Enterococcus gallinarum BM4174. Biochem. J. 301:5-8.
30. Takahashi, S., T. Hirose, M. Matsukawa, T. Shimizu, Y. Kunishima, K. Takeyama, A. Yokoo, H. Hotta, M. Mikami, and T. Tsukamoto. 1999. Analysis of cross infection using genomic fingerprinting in nosocomial urinary tract infection caused by Enterococcus faecalis. J. Infect. Chemother. 5:46-48.
31. Tsai, S.F., M.J. Zervos, D.B. Clewell, S.M. Donabedian, D.F. Sahm, and J.W. Chow. 1998. A new high-level genta-micin resistance gene, aph(2″″)-Id, in Enterococcus2 spp. Anti-microb. Agents Chemother. 42:1229-1232.
32. Vincent, S., R.G. Knight, M. Green, D.F. Sahm, and D.M. Shlaes. 1991. Vancomycin susceptibility and identification of motile enterococci. J. Clin. Microbiol. 29:2335-2337.