Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in Enterococci

Journal of Clinical Microbiology

Volumn 35, Issue 3, 1997, Pages 703-707

  Patel, Robin ^a   Uhl, Jim R ^a   Kohner, Peggy ^a   Hopkins, Marlene K ^a   Cockerill III, Franklin R ^a  

^a MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VANCOMYCIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATION; DNA SEQUENCE; ENTEROCOCCUS; GENE AMPLIFICATION; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS AVIUM; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS GALLINARUM; ENTEROCOCCUS RAFFINOSUS;

EID: 0031057722     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.3.703-707.1997     Document Type: Article

References (23)

1. Centers for Disease Control and Prevention. 1993. Nosocomial enterococci resistant to vancomycin - United States, 1989-1993. Morbid. Mortal. Weekly Rep. 42:597-599.
2. Clark, N. C., R. C. Cooksey, B. C. Hill, J. M. Swenson, and F. C. Tenover. 1993. Characterization of glycopeptide-resistant enterococci from U.S. hospitals. Antimicrob. Agents Chemother. 37:2311-2317.
3. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
4. Dutka-Malen, S., B. Blaimont, G. Wauters, and P. Courvalin. 1994. Emergence of high-level resistance to glycopeptides in Enterococcus gallinarum and Enterococcus casseliflavus. Antimicrob. Agents Chemother. 38:1675-1677.
5. Dutka-Malen, S., S. Evers, and P. Courvalin. 1995. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J. Clin. Microbiol. 33:24-27.
6. Dutka-Malen, S., C. Molinas, M. Arthur, and P. Courvalin. 1990. The VANA glycopeptide resistance protein is related to D-analyl-D-alanine ligase cell wall biosynthesis enzymes. Mol. Gen. Genet. 224:364-372.
7. Dutka-Malen, S., C. Molinas, M. Arthur, and P. Courvalin. 1992. Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine: D-alanine ligase-related protein necessary for vancomycin resistance. Gene 112:53-58.
8. Evers, S., P. E. Reynolds, and P. Courvalin. 1994. Sequence of the vanB and ddl genes encoding D-alanine:D-lactate and D-alanine:D-alanine ligases in vancomycin-resistant Enterococcus faecalis V583. Gene 140:97-102.
9. Evers, S., D. F. Sahm, and P. Courvalin. 1993. The vanB gene of vancomycin-resistant Enterococcus faecalis V583 is structurally related to genes encoding D-Ala:D-Ala ligases and glycopeptide-resistance proteins VanA and VanC. Gene 124:143-144.
10. Handwerger, S., B. Raucher, D. Altarac, J. Monka, S. Marchione, K. V. Singh, B. E. Murray, J. Wolff, and B. Walters. 1993. Nosocomial outbreak due to Enterococcus faecium highly resistant to vancomycin, penicillin, and gentamicin. Clin. Infect. Dis. 16:750-755.
11. Hayden, M. K., G. M. Trenholme, J. E. Schultz, and D. F. Sahm. 1993. In vivo development of teicoplanin resistance in a VanB Enterococcus faecium isolate. J. Infect. Dis. 167:1224-1227.
12. Hospital Infection Control Practices Committee. 1995. Recommendations for preventing the spread of vancomycin resistance. Infect. Control Hosp. Epidemiol. 16:105-113.
13. Jordens, J. Z., J. Bates, and D. T. Griffiths. 1994. Faecal carriage and nosocomial spread of vancomycin-resistant Enterococcus faecium. J. Antimicrob. Chemother. 34:515-528.
14. Karanfil, L. V., M. Murphy, A. Josephson, R. Gaynes, L. Mandel, and B. C. Hill. 1992. A cluster of vancomycin-resistant Enterococcus faecium in an intensive care unit. Infect. Control Hosp. Epidemiol. 13:195-200.
15. Kolbert, C. P., J. E. Connolly, M. J. Lee, and D. H. Persing. 1995. Detection of the staphylococcal mecA gene by chemiluminescent DNA hybridization J. Clin. Microbiol. 33:2179-2182.
16. Leclercq, R., E. Derlot, J. Duval, and P. Courvalin. 1988. Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium. N Engl. J. Med. 319:157-161.
17. Montecalvo, M. A., H. Horowitz, C. Gedris, C. Carbonaro, F. C. Tenover, A. Issah, P. Cook, and G. P. Wormser. 1994. Outbreak of vancomycin-, ampicillin-, and aminoglycoside-resistant Enterococcus faecium bacteremia in an adult oncology unit. Antimicrob. Agents Chemother. 38:1363-1367.
18. National Committee for Clinical Laboratory Standards. 1993. Performance standards for antimicrobial susceptibility testing. M7-A3. National Committee for Clinical Laboratory Standards, Villanova, Pa.
19. Navarro, F., and P. Courvalin. 1994. Analysis of genes encoding D-alanine-D-alanine ligase-related enzymes in Enterococcus casseliflavus and Enterococcus flavescens. Antimicrob. Agents Chemother. 38:1788-1793.
20. Quintiliani, R., S. Evers, and P. Courvalin. 1993. The vanB gene confers various levels of self-transferable resistance to vancomycin in enterococci. J. Infect. Dis. 167:1220-1223.
21. Rice, L. B. 1995. The theoretical origin of vancomycin-resistant enterococci. Clin. Microbiol. Newsl. 17:189-192.
22. Sahm, D. F., L. Free, and S. Handwerger. 1995. Inducible and constitutive expression of vanC-1-encoded resistance to vancomycin in Enterococcus gallinarum. Antimicrob. Agents. Chemother. 39:1480-1484.
23. Uttley, A. H., R. C. George, J. Naidoo, N. Woodford, A. P. Johnson, C. H. Collins, A. J. Gilfillan, L. E. Fitch, and J. Heptonstall. 1989. High-level vancomycin-resistant enterococci causing hospital infections. Epidemiol. Infect. 103:173-181.