Application of 3D tracking, LED illumination and multi-wavelength techniques for quantitative cell analysis in digital holographic microscopy

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 7184, Issue , 2009, Pages

  Kemper, Björn ^a   Kosmeier, Sebastian ^a   Langehanenberg, Patrik ^a   Przibilla, Sabine ^a   Remmersmann, Christian ^a   Stürwald, Stephan ^a   Von Bally, Gert ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

3D tracking; Digital holographic microscopy; LEDs; Multi wavelength digital holography

Indexed keywords

3D TRACKING; AUTO-FOCUS ALGORITHM; AXIAL RESOLUTIONS; CELL ANALYSIS; CELL IMAGING; COHERENCE PROPERTIES; DIGITAL HOLOGRAPHIC MICROSCOPY; FOCUS ADJUSTMENT; INTERFERENCE EFFECTS; LED ILLUMINATION; LEDS; LIVE-CELL IMAGING; MEASUREMENT SETUP; MULTI-FOCUS; MULTI-WAVELENGTH DIGITAL HOLOGRAPHY; MULTIPLE CELLS; MULTIPLE REFLECTIONS; MULTIWAVELENGTH; OBJECT MOVEMENTS; PARTIALLY COHERENT LIGHT; PHASE RESOLUTION; PHASE-CONTRAST IMAGE; PHASE-CONTRAST IMAGING; QUANTITATIVE DYNAMICS; REFLECTIVE SURFACES; SEDIMENTING; TUNABLE LASERS;

COMPUTER GENERATED HOLOGRAPHY; HOLOGRAPHIC INTERFEROMETRY; HOLOGRAPHY; IMAGE ACQUISITION; IMAGE RESOLUTION; LASERS; LIGHT; LIGHT EMITTING DIODES; LIGHTING; MICROLENSES; OBJECT RECOGNITION; PHASE MEASUREMENT;

THREE DIMENSIONAL;

EID: 66649092619     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.808392     Document Type: Conference Paper

References (36)

1. E. Cuche, P. Marquet, and C. Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms," Appl. Opt. 38, 6994-7001 (1999). (Pubitemid 129646711)
2. D. Carl, B. Kemper, G. Wernicke, and G. von Bally, "Parameter- optimized digital holographic microscope for highresolution living-cell analysis," Appl. Opt. 43, 6536-6544 (2004). (Pubitemid 40120770)
3. P. Marquet, B. Rappaz, P. J. Magistretti, E. Cuche, Y. Emery, T. Colomb, and C. Depeursinge, "Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength," Opt. Lett. 30, 468-470 (2005). (Pubitemid 40339483)
4. C. Mann, L. Yu, C. Lo, and M. Kim, "High-resolution quantitative phase-contrast microscopy by digital holography," Opt. Express 13, 8693-8698 (2005). (Pubitemid 41541301)
5. F. Charrière, J. Kühn, T. Colomb, F. Montfort, E. Cuche, Y. Emery, K. Weible, P. Marquet, and C. Depeursinge, "Characterization of microlenses by digital holographic microscopy," Appl. Opt. 45, 829-835 (2006).
6. B. Kemper and G. von Bally, "Digital holographic microscopy for live cell applications and technical inspection," Appl. Opt. 47, A52-A61 (2008).
7. G. von Bally, B. Kemper, D. Carl, S. Knoche, M. Kempe, C. Dietrich, M. Stutz, R. Wolleschensky, K. Schütze, M. Stich, A. Buchstaller, K. Irion, J. Beuthan, I. Gersonde, and J. Schnekenburger, "New Methods for Marker-Free Live Cell and Tumour Analysis," in Biophotonics; Visions for Better Health Care, Jürgen Popp and Martina Strehle, eds. 301-360, VCH-Wiley (2006).
8. L. G. Alexopoulos, G. R. Erickson, and F. Guilak, "A method for quantifying cell size from differential interference contrast images: validation and application to osmotically stressed chondrocytes," J. Microsc. 205, 125-135 (2001). (Pubitemid 34227198)
9. A. Barty, K. A. Nugent, D. Paganin, and A. Roberts, "Quantitative optical phase microscopy," Opt. Lett. 23, 817-819 (1998). (Pubitemid 128590524)
10. G. Popescu, L. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, "Fourier phase microscopy for investigations of biological structures and dynamics," Opt. Lett. 29, 2503-2505 (2004).
11. T. Ikeda, P. Poqesqu, P. Dasari, and M. S. Feld, "Hilbert phase microscopy for investigating fast dynamics in transparent systems," Opt. Lett. 30, 1165-1167 (2005). (Pubitemid 40795095)
12. J. Farinas and A. S. Verkman, "Cell volume and plasma membrane osmotic permeability in epithelial cell layers measured by interferometry," Biophys. J. 71, 3511-3522 (1996). (Pubitemid 26422392)
13. V. P. Tychinskii, "Dynamic phase microscopy: is a 'dialogue' with the cell possible," Physics- Uspekhi 50, 513-528 (2007). (Pubitemid 47395644)
14. A. D. Aguirre, P. Hsiung, T. Ko, I. Hartl, and J. Fujimoto, "High-resolution optical coherence microscopy for highspeed, in vivo cellular imaging," Opt. Lett. 28, 2064-2066, (2003).
15. E. Swanson, J. Izatt, M. Hee, D. Huang, C. Lin, J. Schuman, C. Puliafito, and J. Fujimoto, "In-vivo retinal imaging by optical coherence tomography," Opt. Lett. 18, 1864-1866 (1993).
16. Y. Zhao, Z. Chen, Z. Ding, H. Ren, and J. Nelson, "Real-time phase resolved functional optical coherence tomography by use of optical Hilbert transformation," Opt. Lett. 27, 98-100 (2002). (Pubitemid 34617913)
17. C. G. Rylander, D. P. Davé, T. Akkin, T. E. Milner, K. R. Diller, and A. J. Welch, "Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy," Opt. Lett. 29, 1509-1511 (2004).
18. K. Ellerbee, T. L. Creazzo, and J. A. Izatt, "Investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy, "Opt. Lett. 13, 8115-8124 (2007). (Pubitemid 46988662)
19. C. Joo, K. H. Kim, and J. F. de Boer, "Spectral-domain optical coherence phase and multiphoton microscopy, " Opt. Lett. 32, 623-625 (2007). (Pubitemid 46363456)
20. F. Dubois, L. Johannes, J. C. Legros, "Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence," Appl. Opt. 38, 7085-7094 (1999). (Pubitemid 129646723)
21. B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, "Characterisation of light emitting diodes (LEDs) for application in digital holographic microscopy for inspection of micro and nanostructured surfaces," Opt. Laser Eng. 46, 499-507 (2008).
22. I. Yamaguchi, J. Kato, S. Ohta, J. Mizuno, "Image formation in phase-shifting digital holography and applications to microscopy," Appl. Opt. 40, 6177-6186 (2001).
23. D. Malacara, "Optical shop testing," 501-598, Wiley (1992).
24. M. Liebling, T. Blu, and M. Unser, "Complex-wave retrieval from a single off-axis hologram," J. Opt. Soc. Am. A 21, 367-377 (2004).
25. T. Kreis, Holographic Interferometry: Principles and Methods, Akademie-Publishing, Berlin (1996)
26. B. Rappaz, P. Marquet, E. Cuche, Y. Emery, C. Depeursinge, and P. Magistretti, "Measurement of the integral refractive index and dynamic cell morphotometry of living cells with digital holographic microscopy," Opt. Express 13, 9361-9373 (2005).
27. B. Kemper, D. D. Carl, J. Schnekenburger, I. Bredebusch, M. Schäfer, W. Domschke, and G. von Bally, "Investigation of living pancreas tumor cells by digital holographic microscopy," J. Biomed. Opt. 11, 034005 (2006).
28. N. Lue, G. Popescu, T. Ikeda, R. R. Dasari, K. Badizadegan, and M. S. Feld, "Live cell refractometry using microfluidic devices," Opt. Lett. 31, 2759-2761 (2006). (Pubitemid 44547122)
29. F. Charrière, A. Marian, F. Montfort, J. Kühn, T. Colomb, E. Cuche, P. Marquet, and C. Depeursinge, "Cell refractive index tomography by digital holographic microscopy," Opt. Lett. 31, 178-180 (2006). (Pubitemid 43304068)
30. F. Charrière, N. Pavillon, T. Colomb, C. Depeursinge, T. J. Heger, E. A. D. Mitchell, P. Marquet, and B. Rappaz, "Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba," Opt. Express 14, 7005-7013 (2006). (Pubitemid 44188502)
31. B. Kemper, S. Kosmeier, P. Langehanenberg, G. von Bally, I. Bredebusch, W. Domschke, J. Schnekenburger, "Integral refractive index determination of living suspension cells by multi-focus digital holographic phase contrast Microscopy," J. Biomed. Opt. 054009 (2007).
32. B. Rappaz, F. Charrière, C. Depeursinge, P.J. Magistretti, and P. Marquet, "Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium," Opt. Lett. 33, 744-746 (2007).
33. B. Kemper, P. Langehanenberg, I. Bredebusch, J. Schnekenburger, G. von Bally, "Techniques and applications of digital holographic microscopy for life cell imaging", Proc. SPIE 6633, 66330D (2007).
34. P. Langehanenberg, B. Kemper, D. Dirksen, and G. von Bally, "Autofocusing in digital holographic phase contrast microscopy on pure phase objects for live cell imaging," Appl. Opt. 47, D176-D182 (2008).
35. P. Langehanenberg, B. Kemper, and G. von Bally, "Autofocus Algorithms for digital holographic microscopy", Proc. SPIE 6633, 66330E (2007).
36. F. Dubois, C. Schockaert, N. Callens, and C. Yourassowsky, "Focus plane detection criteria in digital holography microscopy by amplitude analysis," Opt. Express 14, 5895-5908 (2006).