Determination of intracellular cytokines IFN-γ and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture

Canadian Journal of Veterinary Research

Volumn 73, Issue 2, 2009, Pages 137-143

  Papadogiannakis, Emmanouil I ^a   Kontos, Vasilios I ^a   Tamamidou, Maria ^a   Roumeliotou, Anastasia ^a  

^a NATIONAL SCHOOL OF PUBLIC HEALTH   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; CYTOKINE ANTIBODY; GAMMA INTERFERON; INTERLEUKIN 4; IONOMYCIN; MONENSIN; MONOCLONAL ANTIBODY; PHORBOL 13 ACETATE 12 MYRISTATE;

ANIMAL CELL; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE OPTIMIZATION; CYTOKINE PRODUCTION; DOG; FLOW CYTOMETRY; IMMUNOSTIMULATION; INCUBATION TIME; NONHUMAN; T LYMPHOCYTE SUBPOPULATION;

ANIMALS; ANTIGENS, CD4; ANTIGENS, CD8; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; DOGS; DOSE-RESPONSE RELATIONSHIP, IMMUNOLOGIC; FLOW CYTOMETRY; INTERFERON-GAMMA; INTERLEUKIN-4; IONOMYCIN; KINETICS; LYMPHOCYTE ACTIVATION; MONENSIN; TETRADECANOYLPHORBOL ACETATE;

EID: 66549087266     PISSN: 08309000     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Day M. Clinical Immunology of the Dog and Cat. London UK: Manson Publ, 1999.
2. Prussin C, Metcalfe D. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods 1995;188:117-128. (Pubitemid 26011539)
3. Schauer U, Jung T, Krug N, Frew A. Measurement of intracellular cytokines. Immunol Today 1996;17:305-306. (Pubitemid 26201729)
4. Papadogiannakis E, Kontos V, Tamamidou M, Roumeliotou A. Flow cytometry and its applications in veterinary medicine. Journal of the Hellenic Veterinary Medical Society 2005;56:71-77.
5. Ferrer L, Solano-Gallego L, Arboix M, Alberola J. Evaluation of the specific immune response in dogs infected by Leishmania infantum. Vet Dermatol 2000;11:7.
6. Papadogiannakis E. Canine leishmaniosis: A scientific monograph. Athens, Greece: Pashalidis Med Publ, 2007.
7. Collins RA, Howard CJ, Duggan SE, Werling D. Bovine interleukin-12 and modulation of IFN-γ production. Vet Immunol Immunopathol 1999;68:193-207. (Pubitemid 29260006)
8. Pedersen L, Castelruiz Y, Jacobsen S, Aaasted B. Identification of monoclonal antibodies that cross-react with cytokines from different animal species. Vet Immunol Immunopathol 2002;88:111-122. (Pubitemid 34786753)
9. Sewell WAC, North ME, Webster ADB, Farrant J. Determination of intracellular cytokines by flow-cytometry following whole-blood culture. J Immunol Methods 1997;209:67-74. (Pubitemid 28032145)
10. Moore PF, Affolter VK, Olivry T, Schrenzel MD. The use of immunological reagents in defining the pathogenesis of canine skin diseases involving proliferation of leukocytes. In: Kwochka KW, Willemse T, Tscharner CV, eds. Advances in Veterinary Dermatology, vol.3. Oxford, UK: Butterworth Heinemann, 1998:77-94.
11. Jung T, Schauer U, Heusser C, et al. Detection of intracellular cytokines by flow cytometry. J Immunol Methods 1993;159:197-207. (Pubitemid 23071909)
12. Aseenmacher M, Schmitz J, Radbruch A. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: Expression of IL-10 in interferon-γ and in IL-4 expressing cells. Eur J Immunol 1994;24:1097-1101. (Pubitemid 24132241)
13. Openshaw P, Murphy E, Hosken N, et al. Heterogeneity of intracellular cytokine synthesis at the single cell level in polarized T helper 1 and T helper 2 populations. J Exp Med 1995;182:1357-1367.
14. Sopp P, Howard C. IFN-γ and IL-4 production by CD4, CD8 and WC1 γδ TCR+ T cells from cattle lymph nodes and blood. Vet Immunol Immunopathol 2001;81:85-96.
15. Prussin C. Cytokine flow cytometry: Understanding cytokine biology at the single-cell level. J Clin Immunol 1997;17:195-204. (Pubitemid 27261692)
16. Nakayama K, Nakauchi H. Cyclosporin A inhibits the decrease of CD4/CD8 expression induced by protein kinase C activation. Int Immunol 1993;5:419-426. (Pubitemid 23123760)
17. Pelchen MA, Parsons IJ, Marsh M. Phorbol ester-induced down-regulation of CD4 is a multistep process involving dissociation from p56lck, increased association with clathrin-coared pits and altered endosomal sorting. J Exp Med 1993;178:1209-1222. (Pubitemid 23277354)
18. Pala P, Hussell T, Openshaw P. Flow cytometric measurement of intracellular cytokines. J Immunol Methods 2000;243:107-124.
19. Marzio R, Manuel J, Betz-Corradin S. CD69 and regulation of the immune function. Immunopharmacol Immunotoxicol 1999;21:565-582. (Pubitemid 29409367)
20. Ziegler S, Ramsdell F, Alderson M. The activation antigen CD69. Stem Cells 1994;12:456-465. (Pubitemid 24278225)
21. Bank I, Hardan I, Lokshin E, et al. Parenteral administration of an activating monoclonal antibody to the α1β1 integrin in dogs. Immunobiol 2000;202:239-253.
22. Carter LL, Dutton RW. Type 1 and type 2: A fundamental dichotomy for all T cell subsets. Curr Opin Immunol 1996;8:336-342. (Pubitemid 26229654)
23. Papadogiannakis E, Koutinas A, Saridomichelakis M, et al. Cellular immunophenotyping of exfoliative dermatitis in canine leishmaniosis (Leishmania infantum). Vet Immunol Immunopathol 2005;104:227-237.