Determination of intracellular cytokines by flow-cytometry following whole-blood culture

Journal of Immunological Methods

Volumn 209, Issue 1, 1997, Pages 67-74

  Carrock Sewell, W A ^a   North, Margaret E ^a   Webster, A David B ^a   Farrant, John ^a  

^a ROYAL FREE HOSPITAL   (United Kingdom)

Author keywords

Flow cytometry; Intracellular cytokines

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 2; TUMOR NECROSIS FACTOR ALPHA;

ADULT; ARTICLE; BLOOD CULTURE; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MALE; MONONUCLEAR CELL; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION;

ADULT; BLOOD CHEMICAL ANALYSIS; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELLS, CULTURED; CYTOKINES; FEMALE; FLOW CYTOMETRY; HUMANS; INTERFERON TYPE II; INTERLEUKIN-2; INTRACELLULAR FLUID; MALE; STIMULATION, CHEMICAL; TETRADECANOYLPHORBOL ACETATE; TIME FACTORS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0031563905     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00150-6     Document Type: Article

References (13)

1. De Groote D., Zangerle P.F., Gevaert Y., Fassotte M.F., Beguin Y., Noizat-Pirenne F., Pirenne J., Gathy R., Lopez M., Dehart I.et al. Direct stimulation of cytokines (IL-1 beta, TNF-alpha, IL-6, IL-2, IFN-gamma and GM-CSF) in whole blood. I. Comparison with isolated PBMC stimulation. Cytokine. 4:1992;239-248.
2. Elson L.H., Nutman T.B., Metcalfe D.D., Prussin C. Flow cytometric analysis for cytokine production identifies T-helper 1, T-helper 2 and T-helper 0 cells within the human CD4+CD27-lymphocyte subpopulation. J. Immunol. 154:1995;4294-4301.
3. Jung T., Schauer U., Heusser C., Neumann C., Rieger C. Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods. 159:1993;197-207.
4. Kondratenko I., Amlot P.L., Webster A.D.B., Farrant J. Lack of specific antibody response in common variable immunodeficiency (CVID) associated with failure in production of antigen specific memory T-cells. Clin. Exp. Immunol. 108:1997;9-13.
5. Maino V.C., Sunbi M.A., Ruitenberg J.J. Rapid flow cytometric method for measuring lymphocyte subset activation. Cytometry. 20:1995;127-133.
6. Maino V.C., Ruitenberg J.J., Suni M.A. Flow cytometric method for analysis of cytokine expression in clinical samples. Clin. Immunol. Newslett. 16:1996;95-98.
7. North M.E., Ivory K., Funauchi M., Webster A.D.B., Lane A.C., Farrant J. Intracellular cytokine production by human CD4+ and CD8+ T-cells from normal and immunodeficient donors using directly conjugated anti-cytokine antibodies and three-colour flow cytometry. Clin. Exp. Immunol. 105:1996;517-522.
8. Petersen C.M., Christensen E.I., Andresen B.S., Moller B.K. Internalization, lysosomal degradation and new synthesis of surface membrane CD4 in phorbol ester-activated T-lymphocytes and U-937 cells. Exp. Cell Res. 201:1992;160-173.
9. Picker L.J., Singh M.K., Zdraveski Z., Treer J.R., Waldrop S.L., Bergstresser P.R., Maino V.C. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T-cells by flow cytometry. Blood. 86:1995;1408-1419.
10. Prussin C., Metcalfe D.D. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J. Immunol. Methods. 188:1995;117-128.
11. Sander B., Andersson J., Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol. Rev. 119:1991;65-93.
12. Schauer U., Jung T., Krug N., Frew A. Measurement of intracellular cytokines. Immunol. Today. 17:1996;305-306.
13. Thorpe R., Wadhwa M., Bird C.R., Mire-Sluis A.R. Detection and measurement of cytokines. Blood Rev. 6:1992;133-148.