Structures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates

Acta Crystallographica Section D: Biological Crystallography

Volumn 65, Issue 6, 2009, Pages 543-552

  Sukumar, Narayanasami ^a   Dewanti, Asteriani ^b   Merli, Angelo ^c   Rossi, Gian Luigi ^c   Mitra, Bharati ^d   Mathews, F Scott ^e  

^a Department of Obstetrics Gynecology   (United States)

^b WESTERN CAROLINA UNIVERSITY   (United States)

^c UNIVERSITY OF PARMA   (Italy)

^d WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

(S) mandelate dehydrogenase; Flavoenzymes; Membrane proteins

Indexed keywords

ALCOHOL DEHYDROGENASE; BACTERIAL PROTEIN; HYBRID PROTEIN; HYDROXY ACID OXIDASE A; INDOLE DERIVATIVE; L MANDELATE DEHYDROGENASE; L-MANDELATE DEHYDROGENASE; MUTANT PROTEIN; OCTANOL;

ARTICLE; CHEMICAL MODEL; CHEMISTRY; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME REPRESSION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; OXIDATION REDUCTION REACTION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PSEUDOMONAS PUTIDA; X RAY CRYSTALLOGRAPHY;

ALCOHOL OXIDOREDUCTASES; BACTERIAL PROTEINS; CATALYTIC DOMAIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ENZYME REPRESSION; INDOLES; MODELS, CHEMICAL; MUTANT PROTEINS; MUTATION; OCTANOLS; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PSEUDOMONAS PUTIDA; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY;

PSEUDOMONAS PUTIDA;

EID: 66249101973     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S0907444909010270     Document Type: Article

References (37)

1. Blobel, G. (1980). Proc. Natl Acad. Sci. USA, 77, 1496-1500.
2. Brünger, A. T. (1992). Nature (London), 355, 472-475.
3. Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J.-S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T. & Warren, G. L. (1998). Acta Cryst. D54, 905-921.
4. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760-763.
5. Daff, S., Manson, F. D., Reid, G. A. & Chapman, S. K. (1994). Biochem. J. 301, 829-834.
6. DeLano, W. L. (2002). The PyMOL Molecular Graphics System. DeLano Scientific, San Carlos, California, USA.
7. Dewanti, A. R. & Mitra, B. (2003). Biochemistry, 42, 12893-12901.
8. Dewanti, A. R., Xu, Y. & Mitra, B. (2004). Biochemistry, 43, 10692-10700.
9. Emsley, P. & Cowtan, K. (2004). Acta Cryst. D60, 2126-2132.
10. Ghisla, S. & Massey, V. (1989). Eur. J. Biochem. 181, 1-17.
11. Hodel, A., Kim, S.-H. & Brünger, A. T. (1992). Acta Cryst. A48, 851-858.
12. Le, K. H. D. & Lederer, F. (1991). J. Biol. Chem. 266, 20877-20881.
13. Lederer, F. (1991). Chemistry and Biochemistry of the Flavoenzymes, edited by F. Muller, Vol. 2, pp. 153-242. Boca Raton: CRC Press.
14. Lehoux, I. E. & Mitra, B. (1999). Biochemistry, 38, 9948-9955.
15. Lehoux, I. E. & Mitra, B. (2000). Biochemistry, 39, 10055-10065.
16. Lindqvist, Y. (1989). J. Mol. Biol. 209, 151-166.
17. Lindqvist, Y., Brändén, C.-I., Mathews, F. S. & Lederer, F. (1991). J. Biol. Chem. 266, 3198-3207.
18. Maeda-Yorita, K., Aki, K., Sagai, H., Misaki, H. & Massey, V. (1995). Biochimie, 77, 631-642.
19. Merli, A., Brodersen, D. E., Morini, B., Chen, Z., Durley, R. C., Mathews, F. S., Davidson, V. L. & Rossi, G. L. (1996). J. Biol. Chem. 271, 9177-9180.
20. Mitra, B., Gerlt, X A., Babbitt, P. C., Koo, C. W., Kenyon, G. L., Joseph, D. & Petsko, G. A. (1993). Biochemistry, 32, 12959-12967.
21. Morris, A. L., MacArthur, M. W., Hutchinson, E. G. & Thornton, J. M. (1992). Proteins, 12, 345-364.
22. Mowat, C. G., Wehenkel, A., Green, A. J., Walkinshaw, M. D., Reid, G. A. & Chapman, S. K. (2004). Biochemistry, 43, 9519-9526.
23. Murshudov, G. N., Vagin, A. A. & Dodson, E. X (1997). Acta Cryst. D53, 240-255.
24. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
25. Rivetti, C., Mozzarelli, A., Rossi, G. L., Henry, E. R. & Eaton, W. A. (1993). Biochemistry, 32, 2888-2906.
26. Roussel, A. & Cambillau, C. (1989). Silicon Graphics Geometry Partners Directory, pp. 77-78. Silicon Graphics, Mountain View, California USA.
27. Sukumar, N., Dewanti, A. R., Mitra, B. & Mathews, F. S. (2004). J. Biol. Chem. 279, 3749-3757.
28. Sukumar, N., Xu, Y., Gatti, D. L., Mitra, B. & Mathews, F. S. (2001). Biochemistry, 40, 9870-9878.
29. Sun, W., Williams, C. H. Jr & Massey, V (1997). J. Biol. Chem. 272, 27065-27076.
30. Tegoni, M., Begotti, S. & Cambillau, C. (1995). Biochemistry, 34, 9840-9850.
31. Wallace, A. C., Laskowski, R. A. & Thornton, X M. (1995). Protein Eng. 8, 127-134.
32. Xia, Z.-X. & Mathews, F. S. (1990). J. Mol. Biol. 212, 837-863.
33. Xu, Y., Dewanti, A. R. & Mitra, B. (2002). Biochemistry, 41, 12313-12319.
34. Xu, Y & Mitra, B. (1999a). Biochemistry, 38, 12367-12376.
35. Xu, Y & Mitra, B. (1999b). In Flavins and Flavoproteins 1999, edited by S. Ghisla, P. Kroneck, P. Macheroux & H. Sund. Berlin: Agency for Scientific Publications.
36. Yeates, T. O. (1997). Methods Enzymol. 276, 344-358.
37. Yorita, K., Aki, K., Ohkuma-Soyejima, T., Kokubo, T., Misaki, H. & Massey, V (1996). J. Biol. Chem. 271, 28300-28305.