Two potent transactivation domains in the C-terminal region of human NANOG mediate transcriptional activation in human embryonic carcinoma cells

Journal of Cellular Biochemistry

Volumn 106, Issue 6, 2009, Pages 1079-1089

  Do, Hyun Jin ^a,b   Lee, Won Young ^b,c   Lim, Hye Young ^b,d   Oh, Jong Hyun ^a   Kim, Dong Ku ^a,b   Kim, Jin Hoi ^d   Kim, Teoan ^e   Kim, Jae Hwan ^a,b  

^a CHA University   (South Korea)

^b Stem Cell Research Center   (South Korea)

^c Konkuk University   (South Korea)

^d Konkuk University   (South Korea)

^e The Catholic University of Korea   (South Korea)

Author keywords

Acidic residues; Glutamine; Human embryonic carcinoma; Human NANOG; NCCIT; Transactivation domain; Tryptophan repeat

Indexed keywords

GLUTAMINE; TRANSCRIPTION FACTOR NANOG; TRYPTOPHAN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CARCINOGENESIS; CONTROLLED STUDY; EMBRYONAL CARCINOMA STEM CELL; HUMAN; HUMAN CELL; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF; TRANSACTIVATION; TRANSCRIPTION INITIATION; TRANSIENT TRANSFECTION;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; EMBRYONAL CARCINOMA STEM CELLS; GENES, REPORTER; HOMEODOMAIN PROTEINS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; TRANSCRIPTIONAL ACTIVATION;

EID: 66149149780     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.22089     Document Type: Article

References (32)

1. Boiani M, Scholer HR. 2005. Regulatory networks in embryo-derived pluripotent stem cells. Nat Rev Mol Cell Biol 6:872-884.
2. Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, Guenther MG, Kumar RM, Murray HL, Jenner RG, Gifford DK, Melton DA, Jaenisch R, Young RA. 2005. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122:947-956.
3. Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, Smith A. 2003. Functional expression cloning of Nanog, a pluripotency-sustaining factor in embryonic stem cells. Cell 113:643-655.
4. Clark AT. 2007. The stem cell identity of testicular cancer. Stem Cell Rev 3:49-59.
5. Clark AT, Rodriguez RT, Bodnar MS, Abeyta MJ, Cedars MI, Turek PJ, Firpo MT, Reijo Pera RA. 2004. Human STELLAR, NANOG, and GDF3 genes are expressed in pluripotent cells and map to chromosome 12p13, a hotspot for teratocarcinoma. Stem Cells 22:169-179. (Pubitemid 38282632)
6. Damjanov I, Horvat B, Gibas Z. 1993. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. Lab Invest 68:220-232. (Pubitemid 23084295)
7. Do HJ, Lim HY, Kim JH, Song H, Chung HM, Kim JH. 2007. An intact homeobox domain is required for complete nuclear localization of human Nanog. Biochem Biophys Res Commun 353:770-775.
8. Escher D, Bodmer-Glavas M, Barberis A, Schaffner W. 2000. Conservation of glutamine-rich transactivation function between yeast and humans. Mol Cell Biol 20:2774-2782.
9. Hart AH, Hartley L, Ibrahim M, Robb L. 2004. Identification, cloning and expression analysis of the pluripotency promoting Nanog genes in mouse and human. Dev Dyn 230:187-198.
10. Hart AH, Hartley L, Parker K, Ibrahim M, Looijenga LH, Pauchnik M, Chow CW, Robb L. 2005. The pluripotency homeobox gene NANOG is expressed in human germ cell tumors. Cancer 104:2092-2098.
11. Hoei-Hansen CE, Almstrup K, Nielsen JE, Brask Sonne S, Graem N, Skakkebaek NE, Leffers H, Rajpert-De Meyts E. 2005. Stem cell pluripotency factor NANOG is expressed in human fetal gonocytes, testicular carcinoma in situ and germ cell tumours. Histopathology 47:48-56.
12. Jones TD, Ulbright TM, Eble JN, Cheng L. 2004. OCT4: A sensitive and specific biomarker for intratubular germ cell neoplasia of the testis. Clin Cancer Res 10:8544-8547.
13. Jonk LJ, Itoh S, Heldin CH, ten Dijke P, Kruijer W. 1998. Identification and functional characterization of a Smad binding element (SBE) in the JunB promoter that acts as a transforming growth factor-β, activin, and bone morphogenetic protein-inducible enhancer. J Biol Chem 273:21145-21152.
14. Klemsz MJ, Maki RA. 1996. Activation of transcription by PU.1 requires both acidic and glutamine domains. Mol Cell Biol 16:390-397.
15. Kristensen DM, Sonne SB, Ottesen AM, Perrett RM, Nielsen JE, Almstrup K, Skakkebaek NE, Leffers H, Meyts ER. 2008. Origin of pluripotent germ cell tumours: The role of microenvironment during embryonic development. Mol Cell Endocrinol 288:111-118.
16. Kuroda T, Tada M, Kubota H, Kimura H, Hatano SY, Suemori H, Nakatsuji N, Tada T. 2005. Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression. Mol Cell Biol 25:2475-2485.
17. Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. 2003. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell 113:631-642.
18. Mullin NP, Yates A, Rowe AJ, Nijmeijer B, Colby D, Barlow PN, Walkinshaw MD, Chambers I. 2008. The pluripotency rheostat Nanog functions as a dimer. Biochem J 411:227-231.
19. Oh JH, Do HJ, Yang HM, Moon SY, Cha KY, Chung HM, Kim JH. 2005. Identification of a putative transactivation domain in human Nanog. Exp Mol Med 37:250-254.
20. Pan GJ, Pei DQ. 2003. Identification of two distinct transactivation domains in the pluripotency sustaining factor nanog. Cell Res 13:499-502.
21. Pan G, Pei D. 2005. The stem cell pluripotency factor nanog activates transcription with two unusually potent subdomains at its C-terminus. J Biol Chem 280:1401-1407.
22. Pan G, Li J, Zhou Y, Zheng H, Pei D. 2006. A negative feedback loop of transcription factors that controls stem cell pluripotency and self-renewal. FASEB J 20:1730-1732.
23. Pera MF, Trounson AO. 2004. Human embryonic stem cells: Prospect for development. Development 131:5515-5525.
24. Rodda DJ, Chew JL, Lim LH, Loh YH, Wang B, Ng HH, Robson P. 2005. Transcriptional regulation of Nanog by OCT4 and SOX2. J Biol Chem 280:24731-24737.
25. Saikumar P, Murali R, Reddy EP. 1990. Role of tryptophan repeats and flanking amino acids in Myb-DNA interactions. Proc Natl Acad Sci USA 87:8452-8456.
26. Santagata S, Ligon KL, Hornick JL. 2007. Embryonic stem cell transcription factor signatures in the diagnosis of primary and metastatic germ cell tumors. Am J Surg Pathol 31:836-845.
27. Seipel K, Georgiev O, Schaffner W. 1992. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J 11:4961-4968.
28. Stepchenko A, Nirenberg M. 2004. Mapping activation and repression domains of the vnd/NK-2 homeodomain protein. Proc Natl Acad Sci USA 101:13180-13185.
29. Tanaka M, Clouston WM, Herr W. 1994. The Oct-2 glutamine-rich and proline-rich activation domains can synergize with each other or duplicates of themselves to activate transcription. Mol Cell Biol 14:6046-6055.
30. Wang J, Levasseur DN, Orkin SH. 2008. Requirement of Nanog dimerization for stem cell self-renewal and pluripotency. Proc Natl Acad Sci USA 105:6326-6331.
31. Yang HM, Do HJ, Oh JH, Kim JH, Choi SY, Cha KY, Chung HM, Kim JH. 2005. Characterization of putative cis-regulatory elements that control the transcriptional activity of the human Oct4 promoter. J Cell Biochem 96:821-830.
32. Yang HM, Do HJ, Kim DK, Park JK, Chang WK, Chung HM, Choi SY, Kim JH. 2007. Transcriptional regulation of human Oct4 by steroidogenic factor-1. J Cell Biochem 101:1198-1209.