Elucidation of the disulfide bridge pattern of the recombinant human growth and differentiation factor 5 dimer and the interchain Cys/Ala mutant monomer

Analytical Biochemistry

Volumn 390, Issue 2, 2009, Pages 103-108

  Trachsel, Christian ^a   Kämpfer, Urs ^a   Bechtold, Rolf ^b   Schaller, Johann ^a   Schürch, Stefan ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b Biopharm GmbH   (Germany)

Author keywords

Amino acid analysis; Cystine knot structure; Disulfide bridge pattern; Edman degradation; Growth and differentiation factor 5 (GDF5); MALDI TOF MS

Indexed keywords

COVALENT BONDS; CYSTINES; DESORPTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MONOMERS; SULFUR COMPOUNDS;

AMINO ACID ANALYSIS; DISULFIDE BRIDGE; EDMAN DEGRADATION; ELECTROSPRAY MASS SPECTROMETRY; MALDI TOF MS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION TIME OF FLIGHTS; REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TRANSFORMING GROWTH FACTOR BETA;

DIMERS;

CYSTINE; DISULFIDE; GROWTH DIFFERENTIATION FACTOR 5; HOMODIMER; MONOMER; THERMOLYSIN; TRANSFORMING GROWTH FACTOR BETA;

AMINO ACID ANALYSIS; AMINO ACID SEQUENCE; ARTICLE; CYSTINE KNOT MOTIF; DIMERIZATION; DISULFIDE BOND; ELECTROSPRAY MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MUTANT; PRIORITY JOURNAL; PROTEIN DEGRADATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; X RAY DIFFRACTION;

EID: 65849114860     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.04.024     Document Type: Article

References (21)

1. Hötten G., Neidhardt H., Jacobowsky B., and Pohl J. Cloning and expression of recombinant human growth/differentiation factor 5. Biochem. Biophys. Res. Commun. 204 (1994) 646-652
2. Storm E., Huynh T., Copeland N., Jenkins N., Kingsley D., and Lee S.J. Limb alterations in brachypodism mice due to mutations in a new member of the TGF beta superfamily. Nature 368 (1994) 639-643
3. Thomas J., Lin K., Nandedkar M., Camarg M., Cervenka J., and Luyten F. A human chondrodysplasia due to the mutation in a TGF-β superfamily member. Nat. Genet. 12 (1996) 315-317
4. Cheifetz S., Weatherbee J.A., Tsang M.L., Anderson J.K., Mole J.E., Lucas R., and Massague J. The transforming growth factor-β system, a complex pattern of cross-reactive ligands and receptors. Cell 48 (1987) 409-415
5. McDonald N.Q., and Hendrickson W.A. A structural superfamily of growth factors containing a cystine knot motif. Cell 73 (1993) 421-424
6. Daopin S., Piez K.A., Ogawa Y., and Davies D.R. Crystal structure of transforming growth factor-β2: an unusual fold for the superfamily. Science 257 (1992) 369-373
7. Schlunegger M.P., and Grutter M.G. An unusual feature revealed by the crystal structure at 2.2 Å resolution of human transforming growth factor-β2. Nature 358 (1992) 430-434
8. Schreuder H., Liesum A., Pohl J., Kruse M., and Koyama M. Crystal structure of recombinant human growth and differentiation factor 5: evidence for interaction of the type I and type II receptor-binding sites. Biochem. Biophys. Res. Commun. 329 (2005) 1076-1086
9. Nickel J., Kotzsch A., Sebald W., and Mueller T.D. A single residue of GDF-5 defined binding specificity to BMP receptor IB. J. Mol. Biol. 349 (2005) 933-947
10. Schaller J., Kämpfer U., Schürch S., Kuhn-Nentwig L., Häberli S., and Nentwig W. CSTX-9, a toxic peptide from the spider Cupiennius salei: amino acid sequence, disulfide bridge pattern, and comparison with other spider toxins containing the cysteine knot structure. Cell. Mol. Life Sci. 58 (2001) 2115-2121
11. Angeletti R.H., and Bradshaw R.A. Nerve growth factor from mouse submaxillary gland: amino acid sequence. Proc. Natl. Acad. Sci. USA 68 (1971) 2417-2420
12. Hess D., Schaller J., and Rickli E. Identification of the disulfide bonds of human complement C1s. Biochemistry 30 (1991) 2827-2833
13. Wullschleger B., Kuhn-Nentwig L., Tromp J., Kämpfer U., Schaller J., Schürch S., and Nentwig W. CSTX-13, a highly synergistically acting two-chain neurotoxic enhancer in the venom of the spider Cupiennius salei (Ctenidae). Proc. Natl. Acad. Sci. USA 101 (2004) 11251-11256
14. Sieber C., Plöger F., Schwappacher R., Bechtold R., Hanke M., Kawai S., Muraki Y., Katsuura M., Kimura M., Rechtman M.M., Henis Y.I., Pohl J., and Knaus P. Monomeric and dimeric GDF-5 show equal type I receptor binding and oligomerization capability and have the same biological activity. Biol. Chem. 387 (2006) 451-460
15. Honda J., Andou H., Mannen T., and Sugimoto S. Direct refolding of recombinant human growth differentiation factor 5 for large-scale production process. J. Biosci. Bioeng. 89 (2000) 582-589
16. Hötten G.C., Matsumoto T., Kimura M., Bechtold R.F., Kron R., Ohara T., Tanaka H., Satoh Y., Okazaki M., Shirai T., Pan H., Kawai S., Pohl J.S., and Kudo A. Recombinant human growth/differentiation factor 5 stimulates mesenchyme aggregation and chondrogenesis responsible for the skeletal development of limbs. Growth Factors 13 (1996) 65-74
17. Chang J.Y., and Knecht R. Direct analysis of the disulfide content of proteins: methods for monitoring the stability and refolding process of cysteine-containing proteins. Anal. Biochem. 197 (1991) 52-58
18. Bidlingmeyer B.A., Cohen S.A., and Travin T.L. Rapid analysis of amino acids using pre-column derivatization. J. Chromatogr. 336 (1984) 93-104
19. Lu H.S., Klein M.L., Everett R.R., and Lai P.H. Rapid and sensitive determination of protein disulfide bonds. In: L'Italien J. (Ed). Proteins: Structure and Function (1987), Plenum, New York 493-501
20. Martin T., Rösselet S.J., Titani K., and Walsh K.A. Identification of disulfide-bridged substructures within human von Willebrand factor. Biochemistry 26 (1987) 8099-8109
21. Lengweiler S., Schaller J., and Rickli E.E. Identification of disulfide bonds in the ninth component (C9) of human complement. FEBS Lett. 380 (1996) 8-12