Direct refolding of recombinant human growth differentiation factor 5 for large-scale production process

Journal of Bioscience and Bioengineering

Volumn 89, Issue 6, 2000, Pages 582-589

  Honda, Jun ^a   Andou, Hidetoshi ^a   Mannen, Teruhisa ^a   Sugimoto, Shunjiro ^a  

^a Aventis Pharma Ltd   (Japan)

Author keywords

GDF5; Large scale; Protein folding; Purification; TGF

Indexed keywords

BONE; CHROMATOGRAPHY; ESCHERICHIA COLI; FILTRATION; GROWTH KINETICS; PRECIPITATION (CHEMICAL); PURIFICATION; SOLUBILITY;

DIAFILTRATION; DIRECT REFOLDING; INCLUSION BODY; ISOELECTRIC PRECIPITATION; LARGE SCALE PRODUCTION PROCESS; RECOMBINANT HUMAN GROWTH DIFFERENTIATION FACTOR; REFOLDING YIELD; REVERSE PHASE CHROMATOGRAPHY; SOLUBILIZATION; TRANSFORMING GROWTH FACTOR;

PROTEINS;

GROWTH DIFFERENTIATION FACTOR 5; RECOMBINANT PROTEIN;

ARTICLE; CALCULATION; CELL INCLUSION; ESCHERICHIA COLI; GEL FILTRATION; NONHUMAN; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SOLUBILIZATION;

ESCHERICHIA COLI;

EID: 0034210193     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(00)80061-4     Document Type: Article

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