Chapter 14 Real-Time In Vitro Assays For Studying the Role of Chemokines in Lymphocyte Transendothelial Migration Under Physiologic Flow Conditions

Methods in Enzymology

Volumn 461, Issue B, 2009, Pages 311-332

  Shulman, Ziv ^a   Alon, Ronen ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

CELL ADHESION MOLECULE; CHEMOKINE; CYTOKINE; INTEGRIN; LIGAND; REAGENT;

ASSAY; BLOOD VESSEL; BLOOD VESSEL WALL; CELL CULTURE; CELL ISOLATION; CELL LINE; CELL POLARITY; CELL STIMULATION; EFFECTOR CELL; ENDOTHELIUM; ENDOTHELIUM CELL; EQUIPMENT; FORCE; HUMAN; IMMUNOFLUORESCENCE; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; IN VIVO STUDY; INFORMATION; LABORATORY; LEUKOCYTE; LEUKOCYTE ADHERENCE; LEUKOCYTE MIGRATION; LEUKOCYTE MOTILITY; LYMPHOCYTE; MEMBRANE; NONHUMAN; PHYSIOLOGY; PRIORITY JOURNAL; REVIEW; SCANNING ELECTRON MICROSCOPY; SHEAR RATE; SPLEEN CELL; T LYMPHOCYTE; TISSUE CULTURE; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; CELL LINE; CELL MOVEMENT; CHEMOKINES; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; HUMANS; LYMPHOCYTES; MICE; MICROSCOPY, ELECTRON, SCANNING; MODELS, BIOLOGICAL; TUMOR NECROSIS FACTOR-ALPHA;

EID: 65749099416     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)05414-7     Document Type: Review

References (50)

1. Alcaide P., Newton G., Auerbach S., Sehrawat S., Mayadas T.N., Golan D.E., Yacono P., Vincent P., Kowalczyk A., and Luscinskas F.W. p120-catenin regulates leukocyte transmigration through an effect on VE-cadherin phosphorylation. Blood 112 (2008) 2770-2779
2. Astrof N.S., Salas A., Shimaoka M., Chen J., and Springer T.A. Importance of force linkage in mechanochemistry of adhesion receptors. Biochemistry 45 (2006) 15020-15028
3. Barreiro O., Yanez-Mo M., Serrador J.M., Montoya M.C., Vicente-Manzanares M., Tejedor R., Furthmayr H., and Sanchez-Madrid F. Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes. J. Cell. Biol. 157 (2002) 1233-1245
4. Barreiro O., Zamai M., Yanez-Mo M., Tejera E., Lopez-Romero P., Monk P.N., Gratton E., Caiolfa V.R., and Sanchez-Madrid F. Endothelial adhesion receptors are recruited to adherent leukocytes by inclusion in preformed tetraspanin nanoplatforms. J. Cell Biol. 183 (2008) 527-542
5. Bevilacqua M.P. Endothelial-leukocyte adhesion molecules. Annu. Rev. Immunol. 11 (1993) 767-804
6. Carman C.V., Jun C.D., Salas A., and Springer T.A. Endothelial cells proactively form microvilli-like membrane projections upon intercellular adhesion molecule 1 engagement of leukocyte LFA-1. J. Immunol. 171 (2003) 6135-6144
7. Carman C.V., Sage P.T., Sciuto T.E., de la Fuente M.A., Geha R.S., Ochs H.D., Dvorak H.F., Dvorak A.M., and Springer T.A. Transcellular diapedesis is initiated by invasive podosomes. Immunity 26 (2007) 784-797
8. Carr M.W., Alon R., and Springer T.A. The C-C chemokine MCP-1 differentially modulates the avidity of β1 and β2 integrins on T lymphocytes. Immunity 4 (1996) 179-187
9. Cinamon G., Grabovsky V., Winter E., Franitza S., Feigelson S., Shamri R., Dwir O., and Alon R. Novel chemokine functions in lymphocyte migration through vascular endothelium under shear flow. J. Leukoc. Biol. 69 (2001) 860-866
10. Cinamon G., Shinder V., and Alon R. Shear forces promote lymphocyte migration across vascular endothelium bearing apical chemokines. Nat. Immunol. 2 (2001) 515-522
11. 2 integrin occupancy at apical endothelial contacts combine with shear stress signals to promote transendothelial neutrophil migration. J. Immunol. 173 (2004) 7282-7291
12. Cuvelier S.L., and Patel K.D. Shear-dependent eosinophil transmigration on interleukin 4-stimulated endothelial cells: A role for endothelium-associated eotaxin-3. J. Exp. Med. 194 (2001) 1699-1709
13. Ding Z., Xiong K., and Issekutz T.B. Regulation of chemokine-induced transendothelial migration of T lymphocytes by endothelial activation: Differential effects on naive and memory T-cells. J. Leukoc. Biol. 67 (2000) 825-833
14. Ferreira A.M., Isaacs H., Hayflick J.S., Rogers K.A., and Sandig M. The p110delta isoform of PI3K differentially regulates beta1 and beta2 integrin-mediated monocyte adhesion and spreading and modulates diapedesis. Microcirculation 13 (2006) 439-456
15. Hogg N., Laschinger M., Giles K., and McDowall A. T-cell integrins: More than just sticking points. J. Cell. Sci. 116 (2003) 4695-4705
16. Kamata T., Puzon W., and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29). Biochem. J. 305 (1995) 945-951
17. Katagiri K., Imamura M., and Kinashi T. Spatiotemporal regulation of the kinase Mst1 by binding protein RAPL is critical for lymphocyte polarity and adhesion. Nat. Immunol. 7 (2006) 919-928
18. Kim M., Carman C.V., and Springer T.A. Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Science 301 (2003) 1720-1725
19. 2. J. Cell Biol. 167 (2004) 1241-1253
20. Larrivee B., and Karsan A. Isolation and culture of primary endothelial cells. Methods Mol. Biol. 290 (2005) 315-329
21. Laudanna C., and Alon R. Right on the spot. Chemokine triggering of integrin-mediated arrest of rolling leukocytes. Thromb. Haemost. 95 (2006) 5-11
22. Ley K., Laudanna C., Cybulsky M.I., and Nourshargh S. Getting to the site of inflammation: The leukocyte adhesion cascade updated. Nat. Rev. Immunol. 7 (2007) 678-689
23. Luster A.D., Alon R., and von Andrian U.H. Immune cell migration in inflammation: Present and future therapeutic targets. Nat. Immunol. 6 (2005) 1182-1190
24. Mamdouh Z., Chen X., Pierini L.M., Maxfield F.R., and Muller W.A. Targeted recycling of PECAM from endothelial surface-connected compartments during diapedesis. Nature 421 (2003) 748-753
25. Marchesi V. The site of leukocyte emigration during inflammation. Quart. J. Exp. Physiol. 46 (1961) 115-118
26. McGettrick H.M., Filer A., Rainger G.E., Buckley C.D., and Nash G.B. Modulation of endothelial responses by the stromal microenvironment: Effects on leucocyte recruitment. Biochem. Soc. Trans. 35 (2007) 1161-1162
27. Millan J., Hewlett L., Glyn M., Toomre D., Clark P., and Ridley A.J. Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola- and F-actin-rich domains. Nat. Cell Biol. 8 (2006) 113-123
28. Morin N.A., Oakes P.W., Hyun Y.M., Lee D., Chin Y.E., King M.R., Springer T.A., Shimaoka M., Tang J.X., Reichner J.S., and Kim M. Nonmuscle myosin heavy chain IIA mediates integrin LFA-1 de-adhesion during T lymphocyte migration. J. Exp. Med. 205 (2008) 195-205
29. Phillipson M., Heit B., Colarusso P., Liu L., Ballantyne C.M., and Kubes P. Intraluminal crawling of neutrophils to emigration sites: A molecularly distinct process from adhesion in the recruitment cascade. J. Exp. Med. 203 (2006) 2569-2575
30. Piali L., Weber C., LaRosa G., Mackay C.R., Springer T.A., Clark-Lewis I., and Moser B. The chemokine receptor CXCR3 mediates rapid and shear-resistant adhesion-induction of effector T lymphocytes by the chemokines IP10 and Mig. Eur. J. Immunol. 28 (1998) 961-972
31. Ridley A.J., Schwartz M.A., Burridge K., Firtel R.A., Ginsberg M.H., Borisy G., Parsons J.T., and Horwitz A.R. Cell migration: Integrating signals from front to back. Science 302 (2003) 1704-1709
32. Rot A., and von Andrian U.H. Chemokines in innate and adaptive host defense: Basic chemokinese grammar for immune cells. Annu. Rev. Immunol. 22 (2004) 891-928
33. Roth S.J., Carr M.W., Rose S.S., and Springer T.A. Characterization of transendothelial chemotaxis of T lymphocytes. J. Immunol. Methods 188 (1995) 97-116
34. Schenkel A.R., Mamdouh Z., and Muller W.A. Locomotion of monocytes on endothelium is a critical step during extravasation. Nat. Immunol. 5 (2004) 393-400
35. Schreiber T.H., Shinder V., Cain D.W., Alon R., and Sackstein R. Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: Implications for locomotion and the multistep paradigm. Blood 109 (2007) 1381-1386
36. Shaw S.K., Bamba P.S., Perkins B.N., and Luscinskas F.W. Real-time imaging of vascular endothelial-cadherin during leukocyte transmigration across endothelium. J. Immunol. 167 (2001) 2323-2330
37. Shaw S.K., Ma S., Kim M.B., Rao R.M., Hartman C.U., Froio R.M., Yang L., Jones T., Liu Y., Nusrat A., Parkos C.A., and Luscinskas F.W. Coordinated redistribution of leukocyte LFA-1 and endothelial cell ICAM-1 accompany neutrophil transmigration. J. Exp. Med. 200 (2004) 1571-1580
38. Shimonaka M., Katagiri K., Nakayama T., Fujita N., Tsuruo T., Yoshie O., and Kinashi T. Rap1 translates chemokine signals to integrin activation, cell polarization, and motility across vascular endothelium under flow. J. Cell Biol. 161 (2003) 417-427
39. Shulman Z., Shinder V., Klein E., Grabovsky V., Yeger O., Montresor A., Bolomini-Vittori M., Feigelson S.W., Kirchhausen T., Laudanna C., Shakhar G., and Alon R. Lymphocyte crawling and transendothelial migration require chemokine triggering of high affinity LFA-1 integrin. Immunity 30 (2009) 384-396
40. Shulman Z., Pasvolsky R., Woolf E., Grabovsky V., Feigelson S.W., Erez N., Fukui Y., and Alon R. DOCK2 regulates chemokine-triggered lateral lymphocyte motility but not transendothelial migration. Blood 108 (2006) 2150-2158
41. Sikorski E.E., Hallmann R., Berg E.L., and Butcher E.C. The Peyer's patch high endothelial receptor for lymphocytes, the mucosal vascular addressin, is induced on a murine endothelial cell line by tumor necrosis factor-alpha and IL-1. J. Immunol. 151 (1993) 5239-5250
42. Sixt M., Engelhardt B., Pausch F., Hallmann R., Wendler O., and Sorokin L.M. Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis. J. Cell Biol. 153 (2001) 933-946
43. Smith A., Carrasco Y.R., Stanley P., Kieffer N., Batista F.D., and Hogg N. A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes. J. Cell Biol. 170 (2005) 141-151
44. van Buul J.D., Allingham M.J., Samson T., Meller J., Boulter E., Garcia-Mata R., and Burridge K. RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial migration. J. Cell Biol. 178 (2007) 1279-1293
45. Vicente-Manzanares M., and Sanchez-Madrid F. Role of the cytoskeleton during leukocyte responses. Nat. Rev. Immunol. 4 (2004) 1-14
46. Wang S., Voisin M.B., Larbi K.Y., Dangerfield J., Scheiermann C., Tran M., Maxwell P.H., Sorokin L., and Nourshargh S. Venular basement membranes contain specific matrix protein low expression regions that act as exit points for emigrating neutrophils. J. Exp. Med. 203 (2006) 1519-1532
47. Weber K.S., von Hundelshausen P., Clark-Lewis I., Weber P.C., and Weber C. Differential immobilization and hierarchical involvement of chemokines in monocyte arrest and transmigration on inflamed endothelium in shear flow. Eur. J. Immunol. 29 (1999) 700-712
48. Woolf E., Grigorova I., Sagiv A., Grabovsky V., Feigelson S.W., Shulman Z., Hartmann T., Sixt M., Cyster J.G., and Alon R. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces. Nat. Immunol. 8 (2007) 1076-1085
49. Yang L., Froio R.M., Sciuto T., Dvorak A.M., Alon R., and Luscinskas F.W. ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium under flow. Blood 106 (2005) 584-592
50. Zhang H., Schaff U.Y., Green C.E., Chen H., Sarantos M.R., Hu Y., Wara D., Simon S.I., and Lowell C.A. Impaired integrin-dependent function in Wiskott-Aldrich syndrome protein-deficient murine and human neutrophils. Immunity 25 (2006) 285-295