Isolation and regeneration of protoplasts of the moss Physcomitrella patens

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  Cove, David J ^a   Perroud, Pierre François ^a   Charron, Audra J ^a   McDaniel, Stuart F ^a   Khandelwal, Abha ^a   Quatrano, Ralph S ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL REGENERATION; CULTURE MEDIUM; GAMETOPHYTE; ISOLATION PROCEDURE; MOSS; NONHUMAN; PHYSCOMITRELLA; PHYSCOMITRELLA PATENS; PLANT TISSUE; PROTOPLAST CULTURE; PROTOPLAST ISOLATION;

BRYOPHYTA; PHYSCOMITRELLA PATENS;

EID: 65749096265     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5142     Document Type: Article

References (7)

1. Cove, D.J., Perroud, P.-F., Charron, A.J., McDaniel, S.F., Khandelwal, A., and Quatrano, R.S. 2009a. The moss Physcomitrella patens: A novel model system for plant development and genomic studies. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo115.
2. Cove, D.J., Perroud, P.-F., Charron, A.J., McDaniel, S.F., Khandelwal, A., and Quatrano, R.S. 2009b. Culturing the moss Physcomitrella patens. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5136.
3. Cove, D.J., Perroud, P.-F., Charron, A.J., McDaniel, S.F., Khandelwal, A., and Quatrano, R.S. 2009c. Somatic hybridization in the moss Physcomitrella patens using PEG-induced protoplast fusion. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5141.
4. Cove, D.J., Perroud, P.-F., Charron, A.J., McDaniel, S.F., Khandelwal, A., and Quatrano, R.S. 2009d. Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5143.
5. Cove, D.J., Perroud, P.-F., Charron, A.J., McDaniel, S.F., Khandelwal, A., and Quatrano, R.S. 2009e. Transformation of the moss Physcomitrella patens using T-DNA mutagenesis. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5144.
6. Grimsley, N.H., Ashton, N.W., and Cove, D.J. 1977. Complementation analysis of auxotrophic mutants of the moss, Physcomitrella patens, using protoplast fusion. Mol. Gen. Genet. 155: 103-107.
7. Sambrook, J. and Russell, D.W. 2006. Estimation of cell number by hemocytometry counting. Cold Spring Harb. Protoc. doi:10.1101/ pdb.prot4454.