Transformation of the moss Physcomitrella patens using direct DNA uptake by protoplasts

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  Cove, David J ^a   Perroud, Pierre François ^a   Charron, Audra J ^a   McDaniel, Stuart F ^a   Khandelwal, Abha ^a   Quatrano, Ralph S ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MACROGOL;

ARTICLE; CELL KINETICS; CELL TRANSFORMATION; CENTRIFUGATION; DNA TRANSFER; GENE SEQUENCE; MOSS; NONHUMAN; PHYSCOMITRELLA PATENS; PROTOPLAST;

BRYOPHYTA; PHYSCOMITRELLA PATENS;

EID: 65749096839     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5143     Document Type: Article

References (3)

1. Cove, D.J., Perroud, P.-F., Charron, A.J., McDaniel, S.F., Khandelwal, A., and Quatrano, R.S. 2009a. The moss Physcomitrella patens: A novel model system for plant development and genomic studies. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo115.
2. Cove, D.J., Perroud, P.-F., Charron, A.J., McDaniel, S.F., Khandelwal, A., and Quatrano, R.S. 2009b. Isolation and regeneration of protoplasts of the moss Physcomitrella patens. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5140.
3. Sambrook, J. and Russell, D.W. 2006. Estimation of cell number by hemocytometry counting. Cold Spring Harb. Protoc. doi: 10.1101/ pdb.prot4454.