Microinjection of dsRNA into mouse one-cell embryos

Cold Spring Harbor Protocols

Volumn 4, Issue 1, 2009, Pages

  Stein, Paula ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL SURVIVAL; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO; EMBRYO CELL; EMBRYO CULTURE; EMBRYO DEVELOPMENT; EMBRYO TRANSFER; FEMALE; MALE; MICROINJECTION; MOUSE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

EID: 65649105094     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot.5133     Document Type: Article

References (5)

1. Nagy, A., Gertsenstein, M., Vintersten, K., and Behringer, R. 2003. Manipulating the mouse embryo: A laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Nagy, A., Gertsenstein, M., Vintersten, K., and Behringer, R. 2006. Making injection pipettes. Cold Spring Harb. Protoc. doi: 10.1101/ pdb.prot4395.
3. Stein, P. and Svoboda, P. 2006. Microinjection of dsRNA into mouse oocytes and early embryos. Cold Spring Harb. Protoc. doi: 10.1101/ pdb.prot4511.
4. Svoboda, P. 2009. Preparation of dsRNA for microinjection experiments in mouse. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5131.
5. Svoboda, P. and Stein, P. 2009. RNAi experiments in mouse oocytes and early embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.top56.