Microinjection of plasmids into meiotically incompetent mouse oocytes

Cold Spring Harbor Protocols

Volumn 4, Issue 1, 2009, Pages

  Stein, Paula ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO; EMBRYO CELL; EMBRYO CULTURE; EXPRESSION VECTOR; FEMALE; MEIOSIS; MICROINJECTION; MOUSE; NONHUMAN; OOCYTE DEVELOPMENT; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA TRANSCRIPTION;

EID: 65649090219     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5135     Document Type: Article

References (4)

1. Nagy, A., Gertsenstein, M., Vintersten, K., and Behringer, R. 2003. Manipulating the mouse embryo: A laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. Nagy, A., Gertsenstein, M., Vintersten, K., and Behringer, R. 2006. Making injection pipettes. Cold Spring Harb. Protoc. doi: 10.1101/ pdb.prot4395.
3. Svoboda, P. 2009. Cloning a transgene for transgenic RNAi in mouse oocytes. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/ pdb.prot5134.
4. Svoboda, P. and Stein, P. 2009. RNAi experiments in mouse oocytes and early embryos. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.top56.