Knockout studies reveal an important role of Plasmodium lipoic acid protein ligase A1 for asexual blood stage parasite survival

PLoS ONE

Volumn 4, Issue 5, 2009, Pages

  Günther, Svenja ^a,c   Matuschewski, Kai ^b,d   Müller, Sylke ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

^d MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

2 OXOISOVALERATE DEHYDROGENASE (LIPOAMIDE); ACYL COENZYME A; ACYLTRANSFERASE; LIGASE; OCTANOIC ACID; THIOCTIC ACID; THIOCTIC ACID LIGASE; TRANSFERASE; UNCLASSIFIED DRUG; PROTOZOAL PROTEIN;

APICOMPLEXA; APICOMPLEXAN LIFE CYCLE STAGE; APICOPLAST; ARTICLE; ASEXUAL REPRODUCTION; CELL ORGANELLE; ENZYME SUBUNIT; ENZYME SYNTHESIS; ERYTHROCYTE LIFESPAN; GENE DISRUPTION; GENE LOCUS; IN VITRO STUDY; IN VIVO STUDY; MITOCHONDRION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE DEVELOPMENT; PARASITE SURVIVAL; PLASMODIUM; PLASMODIUM BERGHEI; PLASMODIUM FALCIPARUM; PROTEIN TARGETING; AMINO ACID SEQUENCE; ANIMAL; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; ENZYMOLOGY; GENE INACTIVATION; GENE TARGETING; GENETIC TRANSFECTION; GENOTYPE; GROWTH, DEVELOPMENT AND AGING; LIFE CYCLE; METABOLISM; MOLECULAR GENETICS; PARASITE; SEQUENCE ALIGNMENT;

PLASMODIUM BERGHEI; PLASMODIUM FALCIPARUM; RODENTIA;

AMINO ACID SEQUENCE; ANIMALS; CELL SURVIVAL; GENE KNOCKOUT TECHNIQUES; GENE TARGETING; GENOTYPE; LIFE CYCLE STAGES; LIGASES; MOLECULAR SEQUENCE DATA; PARASITES; PLASMODIUM; PROTOZOAN PROTEINS; REPRODUCTION, ASEXUAL; SEQUENCE ALIGNMENT; THIOCTIC ACID; TRANSFECTION;

EID: 65649126040     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0005510     Document Type: Article

References (34)

1. Perham RN (2000) Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions. Annu Rev Biochem 69: 961-1004.
2. Reed LJ, Hackert ML (1990) Structure-function relationships in dihydrolipoamide acyltransferases. J Biol Chem 265: 8971-8974.
3. Douce R, Bourguignon J, Neuburger M, Rébeillé F (2001) The glycine decarboxylase system: a fascinating complex. Trends Plant Sci 6: 167-176.
4. Mooney BP, Miernyk JA, Randall DD (2002) The complex fate of alpha-ketoacids. Annu Rev Plant Biol 53: 357-375.
5. Yasuno R, Wada H (2002) The biosynthetic pathway for lipoic acid is present in plastids and mitochondria in Arabidopsis thaliana. FEBS Lett 517: 110-114.
6. Cronan JE, Zhao X, Jiang Y (2005) Function, attachment and synthesis of lipoic acid in Escherichia coli. Adv Microb Physiol 50: 103-146.
7. Foth BJ, Stimmler LM, Handman E, Crabb BS, Hodder AN, et al. (2005) The malaria parasite Plasmodium falciparum has only one pyruvate dehydrogenase complex, which is located in the apicoplast. Mol Microbiol 55: 39-53.
8. Günther S, McMillan PJ, Wallace LJM, Müller S (2005) Plasmodium falciparum possesses organelle-specific alpha-keto acid dehydrogenase complexes and lipoylation pathways. Biochem Soc Trans 33: 977-980.
9. McMillan PJ, Stimmler LM, Foth BJ, McFadden GI, Müller S (2005) The human malaria parasite Plasmodium falciparum possesses two distinct dihydrolipoamide dehydrogenases. Mol Microbiol 55: 27-38.
10. Thomsen-Zieger N, Schachtner J, Seeber F (2003) Apicomplexan parasites contain a single lipoic acid synthase located in the plastid. FEBS Lett 547: 80-86.
11. Wrenger C, Müller S (2004) The human malaria parasite Plasmodium falciparum has distinct organelle-specific lipoylation pathways. Mol Microbiol 53: 103-113.
12. Günther S, Storm J, Müller S (2009) Plasmodium falciparum: organelle-specific acquisition of lipoic acid. Int J Biochem Cell Biol 41: 748-752.
13. Allary M, Lu JZ, Zhu L, Prigge ST (2007) Scavenging of the cofactor lipoate is essential for the survival of the malaria parasite Plasmodium falciparum. Mol Microbiol 63: 1331-1344.
14. Crawford MJ, Zieger NT, Ray M, Schachtner J, Roos DS, et al. (2006) Toxoplasma gondii scavenges host-derived lipoic acid despite its de novo synthesis in the apicoplast. EMBO J 25: 3214-3222.
15. Mazumdar J, Wilson EH, Masek K, Hunter CA, Striepen B (2006) Apicoplast fatty acid synthesis is essential for organelle biogenesis and parasite survival in Toxoplasma gondii. Proc Natl Acad Sci U S A 103: 13192-13197.
16. Günther S, Wallace L, Patzewitz EM, McMillan PJ, Storm J, et al. (2007) Apicoplast lipoic acid protein ligase B is not essential for Plasmodium falciparum. PLoS Pathog 3: 1938-1949.
17. Witkowski A, Joshi AK, Smith S (2007) Coupling of the de novo fatty acid biosynthesis and lipoylation pathways in mammalian mitochondria. J Biol Chem 282: 14178-14185.
18. Gueguen V, Macherel D, Jaquinod M, Douce R, Bourguignon J (2000) Fatty acid and lipoic acid biosynthesis in higher plant mitochondria. J Biol Chem 275: 5016-5025.
19. Stephens JL, Lee SH, Paul KS, Englund PT (2007) Mitochondrial fatty acid synthesis in Trypanosoma brucei. J Biol Chem 282: 4427-4436.
20. Wu Y, Sifri CD, Lei HH, Su XZ, Wellems TE (1995) Transfection of Plasmodium falciparum within human red blood cells. Proc Natl Acad Sci USA 92: 973-977.
21. Thathy V, Menard R (2002) Gene targeting in Plasmodium berghei. Methods Mol Med 72: 317-331.
22. Le Roch KG, Zhou Y, Blair PL, Grainger M, Moch JK, et al. (2003) Discovery of gene function by expression profiling of the malaria parasite life cycle. Science 301: 1503-1508.
23. O'Riordan M, Moors MA, Portnoy DA (2003) Listeria intracellular growth and virulence require host-derived lipoic acid. Science 302: 462-464.
24. Keeney KM, Stuckey JA, O'Riordan MXD (2007) LplA1-dependent utilization of host lipoyl peptides enables Listeria cytosolic growth and virulence. Mol Microbiol 66: 758-770.
25. Janse CJ, Fayard BF, Mair GR, Ramesar J, Thiel C, et al. (2006) High efficiency transfection of Plasmodium berghei facilitates novel selection procedures. Mol Biochem Parasitol 145: 60-70.
26. Trager W, Jensen JB (1976) Human malaria parasites in continuous culture. Science 193: 673-675.
27. Lambros C, Vanderberg JP (1979) Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol 65: 418-420.
28. Umlas J, Fallon JN (1971) New thick-film technique for malaria diagnosis. Use of saponin stromatolytic solution for lysis. Am J Trop Med Hyg 20: 527-529.
29. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
30. Reed MB, Saliba KJ, Caruana SR, Kirk K, Cowman AF (2000) Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum. Nature 403: 906-909.
31. Tonkin CJ, van Dooren GG, Spurck TP, Struck NS, Good RT, et al. (2004) Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method. Mol Biochem Parasitol 137: 13-21.
32. Crabb BS, Cowman AF (1996) Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum. Proc Natl Acad Sci U S A 93: 7289-7294.
33. Wu Y, Kirkman LA, Wellems TE (1996) Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc Natl Acad Sci U S A 93: 1130-1134.
34. Sambrook J, Fritsch E, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbour, New York, USA: Cold Spring Harbour Press.