A Pyk2-Vav1 complex is recruited to β3-adhesion sites to initiate Rho activation

Biochemical Journal

Volumn 420, Issue 1, 2009, Pages 49-56

  Gao, Chunlei ^a   Blystone, Scott D ^a  

^a SUNY UPSTATE MEDICAL UNIVERSITY   (United States)

Author keywords

3; Adhesion; Integrin; Leucocyte; Phosphorylation; Proline rich tyrosine kinase 2 (Pyk2)

Indexed keywords

ACTIN REORGANIZATION; ADAPTOR PROTEINS; ADHESION SITES; CYTOSKELETAL; DIRECT INTERACTIONS; GREEN FLUORESCENT PROTEIN; IN-CELL; IN-VITRO; INTEGRIN; LEUCOCYTE; NUCLEOTIDE-EXCHANGE FACTOR; PROLINE-RICH TYROSINE KINASE 2; PROLINE-RICH TYROSINE KINASE 2 (PYK2); RECEPTOR TYROSINE KINASE; RECOMBINANT PROTEIN; SIGNALLING MOLECULES; SMALL INTERFERING RNA; TYROSINE PHOSPHORYLATION; VITRONECTIN;

AMINO ACIDS; CELL ADHESION; EMPLOYMENT; NUCLEIC ACIDS; PHOSPHORYLATION; PROTEINS; RNA;

ADHESION;

FOCAL ADHESION KINASE 2; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG; VAV PROTEIN; VAV1 PROTEIN; VITRONECTIN RECEPTOR; BETA3 INTEGRIN; VAV1 PROTEIN, HUMAN;

ACTIN POLYMERIZATION; ARTICLE; BINDING SITE; CELL ADHESION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOSKELETON; ENZYME ACTIVATION; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION; STRESS FIBER; CELL STRAIN K 562; METABOLISM; PHOSPHORYLATION; PROTEIN TRANSPORT;

BINDING SITES; CELL ADHESION; FOCAL ADHESION KINASE 2; HUMANS; INTEGRIN ALPHAVBETA3; INTEGRIN BETA3; K562 CELLS; PHOSPHORYLATION; PROTEIN TRANSPORT; PROTO-ONCOGENE PROTEINS C-VAV; RHO GTP-BINDING PROTEINS;

EID: 65649111192     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20090037     Document Type: Article

References (29)

1. Butler, B. and Blystone, S. D. (2005) Tyrosine phosphorylation of β3 integrin provides a binding site for Pyk2. J. Biol. Chem. 280, 14556-14562
2. Gao, C., Schaefer, E., Lakkis, M. and Blystone, S. D. (2005) β3 tyrosine phosphorylation and αvβ3-mediated adhesion are required for Vav1 association and Rho activation in leukocytes. J. Biol. Chem. 280, 15422-15429
3. Tamas, P., Solti, Z., Bauer, P., Illes, A., Sipeki, S., Bauer, A., Farago, A., Downward, J. and Buday, L. (2003) Mechanism of epidermal growth factor regulation of Vav2, a guanine nucleotide exchange factor for Rac. J. Biol. Chem. 278, 5163-5171
4. Bustelo, X. R. (2000) Regulatory and signaling properties of the Vav family. Mol. Cell. Biol. 20, 1461-1477
5. Movilla, N. and Bustelo, X. R. (1999) Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins. Mol. Cell. Biol. 19, 7870-7885
6. Moores, S. L., Selfors, L. M., Fredericks, J., Breit, T., Fujikawa, K., Alt, F. W., Brugge, J. S. and Swat, W. (2000) Vav family proteins couple to diverse cell surface receptors. Mol. Cell. Biol. 20, 6364-6373
7. Katzav, S., Sutherland, M., Packham, G., Yi, T. and Weiss, A. (1994) The protein tyrosine kinase ZAP-70 can associate with the SH2 domain of proto-Vav. J. Biol. Chem. 269, 32579-32585
8. Fischer, K. D., Kong, Y. Y., Nishina, H., Tedford, K., Marengere, L. E., Kozieradzki, I., Sasaki, T., Starr, M., Chan, G., Gardener, S. et al. (1998) Vav is a regulator of cytoskeletal reorganization mediated by the T-cell receptor. Curr. Biol. 8, 554-562
9. Tybulewicz, V. L., Ardouin, L., Prisco, A. and Reynolds, L. F. (2003) Vav1: a key signal transducer downstream of the TCR. Immunol. Rev. 192, 42-52
10. Tybulewicz, V. L. (2005) Vav-family proteins in T-cell signalling. Curr. Opin. Immunol. 17, 267-274
11. Gakidis, M. A., Cullere, X., Olson, T., Wilsbacher, J. L., Zhang, B., Moores, S. L., Ley, K., Swat, W., Mayadas, T. and Brugge, J. S. (2004) Vav GEFs are required for β2 integrin-dependent functions of neutrophils. J. Cell Biol. 166, 273-282
12. Riteau, B., Barber, D. F. and Long, E. O. (2003) Vav1 phosphorylation is induced by β2 integrin engagement on natural killer cells upstream of actin cytoskeleton and lipid raft reorganization. J. Exp. Med. 198, 469-474
13. Wells, C. M., Bhavsar, P. J., Evans, I. R., Vigorito, E., Turner, M., Tybulewicz, V. and Ridley, A. J. (2005) Vav1 and Vav2 play different roles in macrophage migration and cytoskeletal organization. Exp. Cell Res. 310, 303-310
14. Amarasinghe, G. K. and Rosen, M. K. (2005) Acidic region tyrosines provide access points for allosteric activation of the autoinhibited Vav1 Dbl homology domain. Biochemistry 44, 15257-15268
15. Miletic, A. V., Sakata-Sogawa, K., Hiroshima, M., Hamann, M. J., Gomez, T. S., Ota, N., Kloeppel, T., Kanagawa, O., Tokunaga, M., Billadeau, D. D. and Swat, W. (2006) Vav1 acidic region tyrosine 174 is required for the formation of T cell receptor-induced microclusters and is essential in T cell development and activation. J. Biol. Chem. 281, 38257-38265
16. Zugaza, J. L., Lopez-Lago, M. A., Caloca, M. J., Dosil, M., Movilla, N. and Bustelo, X. R. (2002) Structural determinants for the biological activity of Vav proteins. J. Biol. Chem. 277, 45377-45392
17. Avraham, H., Park, S. Y., Schinkmann, K. and Avraham, S. (2000) RAFTK/ Pyk2-mediated cellular signalling. Cell. Signalling 12, 123-133
18. Xiong, W. C., Macklem, M. and Parsons, J. T. (1998) Expression and characterization of splice variants of PYK2, a focal adhesion kinase-related protein. J. Cell Sci. 111, 1981-1991
19. Ren, X. R., Du, Q. S., Huang, Y. Z., Ao, S. Z., Mei, L. and Xiong, W. C. (2001) Regulation of CDC42 GTPase by proline-rich tyrosine kinase 2 interacting with PSGAP, a novel pleckstrin homology and Src homology 3 domain containing rhoGAP protein. J. Cell Biol. 152, 971-984
20. Duong, L. T., Lakkakorpi, P. T., Nakamura, I., Machwate, M., Nagy, R. M. and Rodan, G. A. (1998) PYK2 in osteoclasts is an adhesion kinase, localized in the sealing zone, activated by ligation of αvβ3 integrin, and phosphorylated by src kinase. J. Clin. Invest. 102, 881-892
21. Duong, L. T. and Rodan, G. A. (1998) Integrin-mediated signaling in the regulation of osteoclast adhesion and activation. Front. Biosci. 3, d757-d768
22. Evangelista, V., Pamuklar, Z., Piccoli, A., Manarini, S., Dell'elba, G., Pecce, R., Martelli, N., Federico, L., Rojas, M., Berton, G. et al. (2007) Src family kinases mediate neutrophil adhesion to adherent platelets. Blood 109, 2461-2469
23. Okigaki, M., Davis, C., Falasca, M., Harroch, S., Felsenfeld, D. P., Sheetz, M. P. and Schlessinger, J. (2003) Pyk2 regulates multiple signaling events crucial for macrophage morphology and migration. Proc. Natl. Acad. Sci. U.S.A. 100, 10740-10745
24. Blystone, S. D., Williams, M. P., Slater, S. E. and Brown, E. J. (1997) Requirement of integrin β3 tyrosine 747 for β3 tyrosine phosphorylation and regulation of αvβ3 avidity. J. Biol. Chem. 272, 28757-28761
25. Blystone, S. D., Graham, I. L., Lindberg, F. P. and Brown, E. J. (1994) Integrin αvβ3 differentially regulates adhesive and phagocytic functions of the fibronectin receptor α5β1. J. Cell Biol. 127, 1129-1137
26. Katagiri, T., Takahashi, T., Sasaki, T., Nakamura, S. and Hattori, S. (2000) Protein-tyrosine kinase Pyk2 is involved in interleukin-2 production by Jurkat T cells via its tyrosine 402. J. Biol. Chem. 275, 19645-19652
27. Miranti, C. K., Leng, L., Maschberger, P., Brugge, J. S. and Shattil, S. J. (1998) Identification of a novel integrin signaling pathway involving the kinase Syk and the guanine nucleotide exchange factor Vav1. Curr. Biol. 8, 1289-1299
28. Gismondi, A., Jacobelli, J., Strippoli, R., Mainiero, F., Soriani, A., Cifaldi, L., Piccoli, M., Frati, L. and Santoni, A. (2003) J. Immunol. 170, 3065-3073
29. Zou, W., Kitaura, H., Reeve, J., Long, F., Tybulewicz, V. L., Shattil, S. J., Ginsberg, M. H., Ross, F. P. and Teitelbaum, S. L. (2007) Syk, c-Src, the αvβ3 integrin, and ITAM immunoreceptors, in concert, regulate osteoclastic bone resorption. J. Cell Biol. 176, 877-888