Establishment of a cell line expressing recombinant factor VII and its subsequent conversion to active form FVIIa through hepsin by genetic engineering method

Vox Sanguinis

Volumn 96, Issue 4, 2009, Pages 309-315

  Halabian, R ^a   Roudkenar, M H ^a   Esmaeili, N S ^a   Masroori, N ^a   Roushandeh, A M ^b   Najafabadi, A J ^c  

^a IRANIAN BLOOD TRANSFUSION ORGANIZATION   (Iran)

^b TABRIZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^c PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

CHO; Co transfection; FVIIa; Genetic engineering; Hepsin; Recombinant

Indexed keywords

BLOOD CLOTTING FACTOR 7; COMPLEMENTARY DNA; HEPSIN; MESSENGER RNA; RECOMBINANT BLOOD CLOTTING FACTOR 7A; THROMBOPLASTIN;

ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; BLOOD CLOTTING TIME; CELL STRAIN HEPG2; CHO CELL; CONTROLLED STUDY; DNA ISOLATION; DNA VECTOR; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE CONSTRUCT; GENE EXPRESSION; GENE ISOLATION; GENETIC ENGINEERING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTHROMBIN TIME; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL LINE, TUMOR; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; DNA, COMPLEMENTARY; ENZYME-LINKED IMMUNOSORBENT ASSAY; FACTOR VII; FACTOR VIIA; GENETIC ENGINEERING; HUMANS; PROTHROMBIN TIME; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 65649088258     PISSN: 00429007     EISSN: 14230410     Source Type: Journal    
DOI: 10.1111/j.1423-0410.2008.01158.x     Document Type: Article

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